LOCUS       sequence-218740-       10151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the MafA trancription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P
  TITLE     Polyswitch lentivectors: ""all-in-one"" lentiviral vectors for 
            drug-inducible gene expression, live selection, and recombination 
            cloning.
  JOURNAL   Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27.
  PUBMED    21761975
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2011-10"; volume: "22(10)"; pages: "1255-67. Epub 
            2011 Sep 27"
COMMENT     Sequence Label: pCWXPG-UBI-MafA
FEATURES             Location/Qualifiers
     source          1..10151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        39..1250
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1087..1106
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1257..1281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1881..1907
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     regulatory      1904..1913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2346..2370)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2387..2897
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2816..2834
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      2913..2922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2919..2921,2922..2924,2925..3638)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2919..2921,2922..2924,2925..3635)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2964..2985)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3225..3244)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3572..3593
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3654..4242
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3707..3727)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4125..4136)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4383..4416)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4436..4616
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4654..4673)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4719..4774
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4773..4792)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5135..5151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5135..5151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5148..5170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5159..5175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5183..5189,5190..5207,5208..5213))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5228..5249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5308..5503
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5354..5489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5509..5643
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5546..5565)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5600..5619
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5711..5728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5882..6469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5962..5981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6640..7431,7432..7500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7263..7282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7501..7605)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7636..8015
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8017..8274
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8321..8446
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8938..9055
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9415..9648
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9833..9877
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caactttgta tagaaaagtt gttaattaag ggtgcagcgg cctccgcgcc gggttttggc
       61 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
      121 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
      181 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
      241 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
      301 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
      361 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
      421 ctgtgatcgt cacttggtga gtagcgggct gctgggctag ccggggcttt cgtggccgcc
      481 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
      541 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
      601 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
      661 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
      721 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
      781 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
      841 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
      901 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
      961 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     1021 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     1081 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     1141 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     1201 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac aggatcccaa
     1261 ctttgtacaa aaaagcaggc ttcaccatgg ccgcggagct ggcgatgggc gccgagctgc
     1321 ccagcagccc gctggccatc gagtacgtca acgacttcga cctgatgaag ttcgaggtga
     1381 agaaggagcc tcccgaggcc gagcgcttct gccaccgcct gccgccaggc tcgctgtcct
     1441 cgacgccgct cagcacgccc tgctcctccg tgccctcctc gcccagcttc tgcgcgccca
     1501 gcccgggcac cggcggcggc ggcggcgcgg ggggcggcgg cggctcgtct caggccgggg
     1561 gcgcccccgg gccgccgagc gggggccccg gcgccgtcgg gggcacctcg gggaagccgg
     1621 cgctggagga tctgtactgg atgagcggct accagcatca cctcaacccc gaggcgctca
     1681 acctgacgcc cgaggacgcg gtggaggcgc tcatcggcag cggccaccac ggcgcgcacc
     1741 acggcgcgca ccacccggcg gccgccgcag cctacgaggc tttccgcggc ccgggcttcg
     1801 cgggcggcgg cggagcggac gacatgggcg ccggccacca ccacggcgcg caccacgccg
     1861 cccaccacca ccacgccgcc caccaccacc accaccacca ccaccatggc ggcgcgggac
     1921 acggcggtgg cgcgggccac cacgtgcgcc tggaggagcg cttctccgac gaccagctgg
     1981 tgtccatgtc ggtgcgcgag ctgaaccggc agctccgcgg cttcagcaag gaggaggtca
     2041 tccggctcaa gcagaagcgg cgcacgctca agaaccgcgg ctacgcgcag tcctgccgct
     2101 tcaagcgggt gcagcagcgg cacattctgg agagcgagaa gtgccaactc cagagccagg
     2161 tggagcagct gaagctggag gtggggcgcc tggccaaaga gcgggacctg tacaaggaga
     2221 aatacgagaa gctggcgggc cggggcggcc ccgggagcgc gggcggggcc ggtttcccgc
     2281 gggagccttc gccgccgcag gccggtcccg gcggggccaa gggcacggcc gacttcttcc
     2341 tgtagaccca gctttcttgt acaaagtggt gataatcgaa ttccacgggg ttggggttgc
     2401 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     2461 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     2521 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     2581 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     2641 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     2701 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     2761 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     2821 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     2881 ccgacctctc tccccagggg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc
     2941 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3001 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3061 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     3121 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3181 ccatgcccga aggctatgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3241 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3301 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3361 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     3421 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     3481 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     3541 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     3601 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgtc gacaatcaac
     3661 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3721 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3781 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3841 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3901 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3961 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4021 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     4081 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4141 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4201 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt cccgcggttc
     4261 gctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4321 gggggactgg aagggctaat tcactcccaa cgaagacaag atgggatcaa ttcaccatgg
     4381 gaataacttc gtatagcata cattatacga agttatgctg ctttttgctt gtactgggtc
     4441 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4501 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4561 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     4621 ctagttgaat cgccagtgtg tcgagctagc agatcttttt ccctctgcca aaaattatgg
     4681 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     4741 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     4801 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     4861 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     4921 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     4981 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     5041 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     5101 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5161 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     5221 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5281 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
     5341 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5401 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5461 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
     5521 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5581 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5641 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5701 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5761 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5821 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5881 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5941 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6001 gctctcctgt tccgaccctg ccgcttacgg atacctgtcc gcctttctcc cttcgggaag
     6061 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6121 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6181 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6241 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6301 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6361 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6421 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6481 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6541 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6601 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6661 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6721 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6781 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6841 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6901 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6961 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7021 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7081 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7141 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7201 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7261 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7321 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7381 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7441 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7501 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7561 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7621 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt
     7681 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     7741 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     7801 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     7861 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     7921 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     7981 agtacatcta cgtattagtc atcgctatta ccatgggagg cgtggcctgg gcgggactgg
     8041 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     8101 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8161 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8221 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     8281 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     8341 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     8401 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8461 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8521 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atactggcct
     8581 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8641 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8701 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8761 aagtaagaaa aaagcacagc aagcagcagg atcttcagac ctggaaattc cctacaatcc
     8821 ccaaagtcaa ggagtagtag aatctatgaa taaagaatta aagaaaatta taggacaggt
     8881 aagagatcag gctgaacatc ttaagacagc agtacaaatg gcagtattca tccacaattt
     8941 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     9001 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     9061 ttattacagg gacagcagaa atccactttg gaaaggacca gcaaagctcc tctggaaagg
     9121 tgaaggggca gtagtaatac aagataatag tgacataaaa gtagtgccaa gaagaaaagc
     9181 aaagatcatt agggattatg gaaaacagat ggcaggtgat gattgtgtgg caagtagaca
     9241 ggatgaggat tagaacatgg aaaagtttag taaaacacca taaggaggag atatgaggga
     9301 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     9361 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     9421 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     9481 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     9541 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     9601 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     9661 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     9721 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     9781 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     9841 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     9901 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     9961 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    10021 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
    10081 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatctagt
    10141 gattatcgga t
//
