LOCUS       sequence-218742-       11825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of Ngn3, PDX1 and MafA proteins from a 
            precursor with self-cleavable 2A peptides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P
  TITLE     Polyswitch lentivectors: ""all-in-one"" lentiviral vectors for 
            drug-inducible gene expression, live selection, and recombination 
            cloning.
  JOURNAL   Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27.
  PUBMED    21761975
REFERENCE   2  (bases 1 to 11825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2011-10"; volume: "22(10)"; pages: "1255-67. Epub 
            2011 Sep 27"
COMMENT     Sequence Label: pCWXPG-UBI-Ngn3PDX1MafA
FEATURES             Location/Qualifiers
     source          1..11825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..31
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             709..762
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1621..1677
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2272..2298
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     regulatory      2295..2304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2770..2794)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2811..3321
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3240..3258
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      3337..3346
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3343..3345,3346..3348,3349..4062)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3343..3345,3346..3348,3349..4059)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3388..3409)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3649..3668)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3996..4017
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4078..4666
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4131..4151)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4549..4560)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4807..4840)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4860..5040
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5078..5097)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5143..5198
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5197..5216)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5559..5575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5559..5575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5572..5594)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5583..5599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5607..5613,5614..5631,5632..5637))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5652..5673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5732..5927
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5778..5913
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5933..6067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5970..5989)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6024..6043
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6135..6152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6306..6893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6386..6405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7064..7855,7856..7924))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7687..7706
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7925..8029)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8060..8439
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8441..8698
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8745..8870
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9362..9479
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9839..10072
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10257..10301
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    10576..10596
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        10614..11825
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     11662..11681
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 aggatcccaa ctttgtacaa aaaagcaggc tggcgccgga accaattcag tcgatccatg
       61 acgcctcaac cctcgggtgc gcccactgtc caagtgaccc gtgagacgga gcggtccttc
      121 cccagagcct cggaagacga agtgacctgc cccacgtccg ccccgcccag ccccactcgc
      181 acacggggga actgcgcaga ggcggaagag ggaggctgcc gaggggcccc gaggaagctc
      241 cgggcacggc gcgggggacg cagccggcct aagagcgagt tggcactgag caagcagcga
      301 cggagtcggc gaaagaaggc caacgaccgc gagcgcaatc gaatgcacaa cctcaactcg
      361 gcactggacg ccctgcgcgg tgtcctgccc accttcccag acgacgcgaa gctcaccaag
      421 atcgagacgc tgcgcttcgc ccacaactac atctgggcgc tgactcaaac gctgcgcata
      481 gcggaccaca gcttgtacgc gctggagccg ccggcgccgc actgcgggga gctgggcagc
      541 ccaggcggtt cccccgggga ctgggggtcc ctctactccc cagtctccca ggctggcagc
      601 ctgagtcccg ccgcgtcgct ggaggagcga cccgggctgc tgggggccac ctcttccgcc
      661 tgcttgagcc caggcagtct ggctttctca gattttctgg gaagcggtga gggcagagga
      721 agcttgctaa cttgtggaga tgtggaagag aatccaggac ctatgaacgg cgaggagcag
      781 tactacgcgg ccacgcagct ttacaagggc ccatgcgcgt tccagcgagg cccggcgccg
      841 gagttcagcg ccagcccccc tgcgtgcctg tacatgggcc gccagccccc gccgccgccg
      901 ccgcacccgt tccctggcgc cctgggcgcg ctggagcagg gcagcccccc ggacatctcc
      961 ccgtacgagg tgccccccct cgccgacgac cccgcggtgg cgcaccttca ccaccacctc
     1021 ccggctcagc tcgcgctccc ccacccgccc gccgggccct tcccggaggg agccgagccg
     1081 ggcgtcctgg aggagcccaa ccgcgtccag ctgcctttcc catggatgaa gtctaccaaa
     1141 gctcacgcgt ggaaaggcca gtgggcaggc ggcgcctacg ctgcggagcc ggaggagaac
     1201 aagcggacgc gcacggccta cacgcgcgca cagctgctag agctggagaa ggagttccta
     1261 ttcaacaagt acatctcacg gccgcgccgg gtggagctgg ctgtcatgtt gaacttgacc
     1321 gagagacaca tcaagatctg gttccaaaac cgccgcatga agtggaaaaa ggaggaggac
     1381 aagaagcgcg gcggcgggac agctgtcggg ggtggcgggg tcgcggagcc tgagcaggac
     1441 tgcgccgtga cctccggcga ggagcttctg gcgctgccgc cgccgccgcc ccccggaggt
     1501 gctgtgccgc ccgctgcccc cgttgccgcc cgagagggcc gcctgccgcc tggccttagc
     1561 gcgtcgccac agccctccag cgtcgcgcct cggcggccgc aggaaccacg aggatcagga
     1621 gccaccaact tcagtttgct aaagcaagct ggagacgtcg aggagaatcc aggaccaatg
     1681 gccgcggagc tggcgatggg cgccgagctg cccagcagcc cgctggccat cgagtacgtc
     1741 aacgacttcg acctgatgaa gttcgaggtg aagaaggagc ctcccgaggc cgagcgcttc
     1801 tgccaccgcc tgccgccagg ctcgctgtcc tcgacgccgc tcagcacgcc ctgctcctcc
     1861 gtgccctcct cgcccagctt ctgcgcgccc agcccgggca ccggcggcgg cggcggcgcg
     1921 gggggcggcg gcggctcgtc tcaggccggg ggcgcccccg ggccgccgag cgggggcccc
     1981 ggcgccgtcg ggggcacctc ggggaagccg gcgctggagg atctgtactg gatgagcggc
     2041 taccagcatc acctcaaccc cgaggcgctc aacctgacgc ccgaggacgc ggtggaggcg
     2101 ctcatcggca gcggccacca cggcgcgcac cacggcgcgc accacccggc ggccgccgca
     2161 gcctacgagg ctttccgcgg cccgggcttc gcgggcggcg gcggagcgga cgacatgggc
     2221 gccggccacc accacggcgc gcaccacgcc gcccaccacc accacgccgc ccaccaccac
     2281 caccaccacc accaccatgg cggcgcggga cacggcggtg gcgcgggcca ccacgtgcgc
     2341 ctggaggagc gcttctccga cgaccagctg gtgtccatgt cggtgcgcga gctgaaccgg
     2401 cagctccgcg gcttcagcaa ggaggaggtc atccggctca agcagaagcg gcgcacgctc
     2461 aagaaccgcg gctacgcgca gtcctgccgc ttcaagcggg tgcagcagcg gcacattctg
     2521 gagagcgaga agtgccaact ccagagccag gtggagcagc tgaagctgga ggtggggcgc
     2581 ctggccaaag agcgggacct gtacaaggag aaatacgaga agctggcggg ccggggcggc
     2641 cccgggagcg cgggcggggc cggtttcccg cgggagcctt cgccgccgca ggccggtccc
     2701 ggcggggcca agggcacggc cgacttcttc ctgtagcgac gaattcgcgg ccgcactcga
     2761 gatatctaga cccagctttc ttgtacaaag tggtgataat cgaattccac ggggttgggg
     2821 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     2881 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     2941 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     3001 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     3061 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     3121 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     3181 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     3241 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     3301 atcaccgacc tctctcccca gggggatcca ccggtcgcca ccatggtgag caagggcgag
     3361 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     3421 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     3481 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     3541 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     3601 tccgccatgc ccgaaggcta tgtccaggag cgcaccatct tcttcaagga cgacggcaac
     3661 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     3721 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     3781 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     3841 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     3901 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     3961 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     4021 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgtcgacaat
     4081 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4141 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4201 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4261 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4321 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4381 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4441 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     4501 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4561 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4621 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcccgcg
     4681 gttcgcttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     4741 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatggga tcaattcacc
     4801 atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg
     4861 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4921 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4981 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5041 gcatctagtt gaatcgccag tgtgtcgagc tagcagatct ttttccctct gccaaaaatt
     5101 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     5161 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     5221 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     5281 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     5341 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     5401 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     5461 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     5521 gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     5581 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5641 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5701 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc
     5761 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5821 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5881 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt
     5941 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6001 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6061 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6421 gtgcgctctc ctgttccgac cctgccgctt acggatacct gtccgccttt ctcccttcgg
     6481 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6541 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6601 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6661 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6721 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6781 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6841 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6901 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6961 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7021 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7081 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7141 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7201 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7261 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7321 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7381 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7441 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7501 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7561 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7621 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7681 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7741 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7801 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7861 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7921 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7981 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8041 gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta atcaattacg
     8101 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     8161 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     8221 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     8281 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     8341 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     8401 tggcagtaca tctacgtatt agtcatcgct attaccatgg gaggcgtggc ctgggcggga
     8461 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     8521 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8581 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8641 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8701 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     8761 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     8821 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     8881 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     8941 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatactg
     9001 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     9061 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     9121 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     9181 acaaaagtaa gaaaaaagca cagcaagcag caggatcttc agacctggaa attccctaca
     9241 atccccaaag tcaaggagta gtagaatcta tgaataaaga attaaagaaa attataggac
     9301 aggtaagaga tcaggctgaa catcttaaga cagcagtaca aatggcagta ttcatccaca
     9361 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9421 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9481 gggtttatta cagggacagc agaaatccac tttggaaagg accagcaaag ctcctctgga
     9541 aaggtgaagg ggcagtagta atacaagata atagtgacat aaaagtagtg ccaagaagaa
     9601 aagcaaagat cattagggat tatggaaaac agatggcagg tgatgattgt gtggcaagta
     9661 gacaggatga ggattagaac atggaaaagt ttagtaaaac accataagga ggagatatga
     9721 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     9781 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     9841 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     9901 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     9961 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
    10021 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
    10081 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
    10141 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
    10201 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
    10261 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
    10321 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
    10381 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
    10441 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
    10501 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
    10561 tagtgattat cggatcaact ttgtatagaa aagttgttaa ttaagggtgc agcggcctcc
    10621 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc
    10681 agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg
    10741 ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga
    10801 cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta
    10861 gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata
    10921 taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt
    10981 cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg
    11041 gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc
    11101 tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa
    11161 atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt
    11221 gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat
    11281 gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga
    11341 agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg
    11401 gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt
    11461 cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg
    11521 cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg
    11581 cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat
    11641 gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga
    11701 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
    11761 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
    11821 tagac
//
