LOCUS       sequence-218743-        7957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Galactose-inducible L-BC gag: cure killer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Bilanchone V, Clemens K, Kaake R, Dawson AR, Matheos D, Nagashima K,
            Sitlani P, Patterson K, Chang I, Huang L, Sandmeyer S
  TITLE     Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to 
            Infect New Genomes.
  JOURNAL   PLoS Genet. 2015 Sep 30;11(9):e1005528. doi: 
            10.1371/journal.pgen.1005528. eCollection 2015.
  PUBMED    26421679
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2015-09-30"; volume: "11(9):e1005528. doi"; pages: "
            10.1371/journal.pgen.1005528. eCollection 2015"
COMMENT     Sequence Label: pVB3011
FEATURES             Location/Qualifiers
     source          1..7957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2..119
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     414..437
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     475..494
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2706..2953
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2722..2740)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3030..3047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3201..3789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3281..3300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3960..4751,4752..4820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4583..4602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(4916..5719)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5720..5935)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      6536..7413
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(7424..7937)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7619..7640)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7831..7850
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagcttggta ccaaccggaa ttatgtcgtc tctgttaaat
      541 tcattactac cagaatattt taaacctaaa actaatttga atatcaactc ttctagggtc
      601 caatatggct ttaatgctcg cattgatatg cagtatgaag acgatagtgg gactagaaaa
      661 ggctcaagac ccaatgcatt tatgtctaac acagttgctt ttataggaaa ctatgaaggt
      721 attattgttg atgacattcc gatattggat ggtcttaggg ccgacatttt tgatactcat
      781 ggtgacttag acatgggcct cgttgaggat gcattgtcta agagtaccat gattagaagg
      841 aatgtaccaa cttatactgc ttacgcgagt gaattactgt ataagagaaa ccttacatct
      901 ctattttaca atatgctccg tttatactac attaaaaaat ggggcagtat taagtatgaa
      961 aagatgccat cttttatgat aatggccacg cctgtctttt aaacaggcaa ttgtttccaa
     1021 agtctcgtga tgcttctttg gaatcaagcc tctctttacc tgaggctgaa attgcaatgc
     1081 ttgatcctgg cctggaattt ccagaagagg atgtgcctgc aattttatgg cacggcagag
     1141 tgtcatccag agcaacgtgt atcttagggc aagcttgctc agagttcgcg cctctggccc
     1201 ccttttcgat tgcgcattat tcaccacaat tgacgagaaa actatttgtc aatgcgcccg
     1261 ctgggattga gcctagctcc gggcggtata ctcacgagga tgtaaaagat gcgattacga
     1321 tccttgtgtc tgcaaaccag gcttatactg actttgaggc agcatacttg atgcttgctc
     1381 aaacgttggt ctcacctgta ccacgcactg ccgaagcaag tgcatggttc atcaatgctg
     1441 gcatggtcaa tatgccaact ttgtcatgtg caaatggtta ttatccagca ctgaccaatg
     1501 tcaatcctta ccaccggcta gacacatgga aagatacgtt aaatcattgg gtggcttatc
     1561 ccgacatgct gttttaccat tcagtggcaa tgattgagag ctgctatgtt gaactcggga
     1621 atgtggctcg tgtgtcagac agtgatgcaa taaacaaata cactttcact gagctatcag
     1681 tgcaaggacg gcctgttatg aatcgaggaa ttattgtaga tctgacactt gtggcaatgc
     1741 gtactggtag ggagatctca ctaccttacc cggtcagctg tggcctgacc cgtacagacg
     1801 cgttattgca aggtactgag attcacgttc cagttgttgt caaagatatt gacatgcccc
     1861 agtattacaa cgcgattgat aaggatgtta ttgaggggca ggaaactgtg attagagtga
     1921 aacagctgcc accagctatg tatccaattt atacttacgg catcaacact actgaattct
     1981 attctgacca ttttgaagac caggtacaag ttgaaatggc accaatcgat aatggaaaag
     2041 cagtttttaa cgatgcaaga aagttttcga aatttatgtc cataatgcgc atgatgggga
     2101 atgatgttac tgctactgat ttagttacag gtagaaaagt gtcgaattgg gccgacaact
     2161 catcagggcg tttcttgtac acggatgtga agtatgaagg acaaactgcc tttttggttg
     2221 atatggatac tgtcaaggcg agagaccact gttgggtgtc aattgttgat cctaatggta
     2281 caatgaactt gtcatataag atgaccaatt ttagagcagc gatgttttct agaaacaagc
     2341 ccttgtatat gacagggggg tcagtcagga ccatagctac tggcaattat cgagatgctg
     2401 ctgaaagatt gcgtgcaatg gatgaaacgc tcagattaaa accttttaag attactgaga
     2461 agttggattt tcgtgtagca gcttacgcga taccaagttt gtcgggcagc aatatgccat
     2521 ccttacacca tcaggaacaa ctacagatat cagaagtgga cgcggaacca atcaatccta
     2581 taggagagga cgaacttcca ccggatatag aatagggatc cactagtaac ggccgccagt
     2641 gtgctggaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg
     2701 ccgcatcatg taattagtta tgtcacgctt acattcacgc cctcccccca catccgctct
     2761 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt
     2821 tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg
     2881 cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa
     2941 ggctttaatt tgcaagctgc ggccctgcat taatgaatcg gccaacgcgc ggggagaggc
     3001 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3061 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3601 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4561 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4801 cggaaatgtt gaatactcat actcttcctt tttcaatggg taataactga tataattaaa
     4861 ttgaagctct aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt
     4921 tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct
     4981 ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc
     5041 ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat
     5101 ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt
     5161 ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac
     5221 ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa
     5281 ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg
     5341 ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg
     5401 cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta
     5461 aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat
     5521 cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta
     5581 actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt
     5641 cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct
     5701 tatatgtagc tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt
     5761 gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca
     5821 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa
     5881 aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaagct
     5941 agcttatcga tgataagctg tcaaacatga gaattaattc cacggactat agactatacc
     6001 tagtatactc cgtctactgt acgatacact tccgctcagg tccttgtcct ttaacgaggc
     6061 cttaccactc ttttgttact ctattgatcc agctcagcaa aggcagtgtg atctaagatt
     6121 ctatcttcgc gatgtagtaa aactagctag accgagaaag agactagaaa tgcaaaaggc
     6181 acttctacaa tggctgccat cattattatc cgatgtgacg ctgcagcttc tcaatgatat
     6241 tcgaatacgc tttgaggaga tacagcctaa tatccgacaa actgttttac agatttacga
     6301 tcgtacttgt tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac
     6361 agatagtata tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt
     6421 atgtatttcg gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc
     6481 atccccggtt cattttctgc gtttccatct tgcacttcaa tagcatatct ttgttaacga
     6541 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     6601 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     6661 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     6721 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     6781 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     6841 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     6901 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     6961 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     7021 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     7081 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     7141 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     7201 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     7261 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     7321 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     7381 ttgagcaatg tttgtggaag cggtattcgc aatgggaagc tccaccccgg ttgataatca
     7441 gaaaagcccc aaaaacagga agattgtata agcaaatatt taaattgtaa gcgttaatat
     7501 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     7561 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     7621 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     7681 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     7741 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     7801 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     7861 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     7921 gccgctacag ggcgcgtggg gatcgatcca ctagtcc
//
