LOCUS       sequence-218745-       10172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  alpha factor inducible Ty3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Bilanchone V, Clemens K, Kaake R, Dawson AR, Matheos D, Nagashima K,
            Sitlani P, Patterson K, Chang I, Huang L, Sandmeyer S
  TITLE     Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to 
            Infect New Genomes.
  JOURNAL   PLoS Genet. 2015 Sep 30;11(9):e1005528. doi: 
            10.1371/journal.pgen.1005528. eCollection 2015.
  PUBMED    26421679
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2015-09-30"; volume: "11(9):e1005528. doi"; pages: "
            10.1371/journal.pgen.1005528. eCollection 2015"
COMMENT     Sequence Label: pTD3685
FEATURES             Location/Qualifiers
     source          1..10172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    69..572
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        609..713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(714..782,783..1574)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(932..951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1745..2333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2234..2253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2487..2504
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2621..2642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2657..2662,2663..2680,2681..2687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2695..2711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2700..2722
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2719..2735
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2719..2735
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2756..2774
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8191..8210)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8192..8210)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8217..8234)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8217..8233)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8226..8248)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8374..8829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8461..8480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8671..8692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8960..9763)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9764..9979)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(10003..10022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10122..10144
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
      121 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta
      181 aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa
      241 tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag
      301 atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt
      361 ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa
      421 aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta
      481 ttttttcttt aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa
      541 tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg
      601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1861 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     2641 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     2701 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta
     2761 accctcacta aagggaacaa aagctgtcga gacggtatcg ataagctttg ttgtatctca
     2821 aaatgagata tgtcagtatg acaatacgtc accctgaacg ttcataaaac acatatgaaa
     2881 caaccttata acaaaacgaa caacatgaga caaaacccga ccttccctag ctgaactacc
     2941 caaagtataa atgcctgaac aattagttta gatccgagat tccgcgcttc caccacttag
     3001 tatgattcat attttatata atatataaga taagtaacat tccgtgaatt aatctgataa
     3061 actgttttga caactggtta cttccctaag actgtttata ttaggattgt caagacactc
     3121 cggtattact cgagcccgta atacaacacc tggtagcgtt aaaggttact aattgttcaa
     3181 acgaaccatc gaaaagccga acctagctac accacacccc agtatgagct ttatggatca
     3241 aatcccagga ggaggaaatt atccaaaact cccagtagaa tgccttccta acttcccgat
     3301 ccaaccatct ttgaccttca gaggtagaaa tgactcgcat aaactgaaaa actttatctc
     3361 cgaaataatg ttaaacatgt ctatgatatc ttggccgaat gatgccagtc gtattgtgta
     3421 ctgcagaaga catttattaa accccgctgc tcagtgggct aatgactttg tacaagaaca
     3481 aggtatactt gaaataacat tcgacacatt catacaagga ttatatcagc atttctataa
     3541 gccaccagat atcaataaaa tctttaatgc aatcacgcaa ctttccgaag ctaaacttgg
     3601 tattgagcgt ctcaaccaac gattcagaaa gatttgggac agaatgccac cagacttcat
     3661 gaccgaaaaa gctgccataa tgacatatac taggctattg acaaaggaaa cctataatat
     3721 tgtcagaatg cacaaaccag agacattaaa agacgccatg gaagaggctt accagacaac
     3781 tgcactaact gaaagattct tcccaggatt cgaacttgat gctgatggag acactatcat
     3841 cggtgccaca acccacttac aagaagaata cgactctgac tatgattcag aagataatct
     3901 gacccagaat ggatacgtcc ataccgtaag gacaagaaga tcttacaata aaccaatgtc
     3961 aaatcatcga aacaggagaa ataacaaccc atctagagaa gaatgtataa aaaatcggct
     4021 atgcttctat tgtaagaaag agggacatcg cctgaacgaa tgtagagcac gtaaggcgag
     4081 ttctaaccga tcttgaactc gaatcaaaag accaacaaac tccttttatc aaaaccttac
     4141 caattgtaca ctatatcgcc atccccgaga tggacaatac cgccgaaaaa accataaaaa
     4201 tacaaaacac gaaagtaaaa accctgtttg acagtggatc acccacgtca tttatccgaa
     4261 gagatattgt agaacttctc aaatacgaaa tctacgagac ccctccactc cgttttagag
     4321 gattcgtagc caccaaatcc gccgttacat ccgaagcagt caccattgac ctcaaaatca
     4381 atgacctgca tataacttta gccgcgtaca tactggataa catggactac caattgttaa
     4441 ttggaaatcc aatcttacgc cgctacccga aaatcctgca cacagtactg aataccagag
     4501 agagccccga ctccttaaag cccaagactt atcgctccga aaccgttaat aacgttagaa
     4561 cctactccgc tggtaatcgt ggtaacccca gaaacataaa actgtctttt gcccccacca
     4621 ttctcgaagc aactgacccg aaatccgctg gtaatcgtgg tgactccaga accaaaaccc
     4681 tgtctcttgc aaccactact cctgcagcaa ttgacccgct tacgaccctt gataacccag
     4741 gtagtactca aagtacattt gcgcaattcc cgatacctga agaagcgagc atcctagaag
     4801 aggatggaaa atactccaac gttgtctcaa ccattcagag tgtagaacct aatgctactg
     4861 atcacagcaa taaggacacc ttttgcactt tgccagtttg gttacaacag aagtatagag
     4921 agatcatacg taatgatctc ccaccaagac ctgccgacat taataacatc cccgtaaaac
     4981 atgatattga aattaaacct ggcgcaagac tacctcgact acagccatac catgttacag
     5041 aaaagaacga acaagaaatc aacaaaatag ttcaaaaact gctcgataac aagttcattg
     5101 ttccctcaaa gtcgccttgc agctcccctg tagtcctcgt cccgaagaaa gacggtacct
     5161 tccgactctg cgtcgattac cgcaccctga acaaagctac catctccgac ccattcccat
     5221 tacccagaat cgacaaccta ttgagccgta ttggaaatgc ccagatattt accacgctag
     5281 atttgcatag tggttaccac cagatcccga tggaacccaa agaccgctac aaaaccgcct
     5341 ttgtcacacc atccggtaag tatgaatata ccgtcatgcc atttggctta gtcaatgcac
     5401 ctagtacatt cgcaagatac atggctgata catttagaga cctgagattc gtcaatgttt
     5461 accttgatga tatattaata ttctccgaat ctccagaaga acattggaaa catttagaca
     5521 cggtactaga aagattaaag aacgagaacc tcattgttaa gaagaaaaaa tgtaaatttg
     5581 catctgaaga aactgagttt ttaggctata gtattggaat ccagaaaata gctccactac
     5641 agcacaaatg tgcagcaatc cgagactttc cgacgcctaa aacagtaaaa caagcacaga
     5701 gatttttagg aatgattaat tactacagac gattcattcc aaattgctcc aagattgcac
     5761 agccaatcca actgtttatt tgtgacaaaa gtcaatggac agaaaaacaa gacaaggcaa
     5821 ttgataaact aaaagacgcc ttgtgtaact cccccgtcct agtaccattc aacaacaaag
     5881 caaactaccg acttacaaca gacgcctcaa aagacggcat tggtgctgtt ctagaagaag
     5941 tcgacaacaa gaacaaactt gttggtgtcg tcggttactt ctctaaatcc ttagagagtg
     6001 cccagaaaaa ctatcctgct ggcgaattag aactacttgg aattatcaaa gcactccacc
     6061 acttccgata tatgcttcac ggaaagcatt tcacgttaag aacagaccac attagtttgt
     6121 tatcattaca aaacaagaac gaacccgcac gacgcgtgca acgctggtta gatgacctag
     6181 ccacatatga cttcacctta gaatacctag ctggacccaa gaacgttgtc gcagatgcca
     6241 tatcccgtgc cgtatatact ataacccccg aaacatcccg acctatcgac acagaaagct
     6301 ggaaatctta ctacaaatca gacccattat gtagtgctgt cttaattcat atgaaagaat
     6361 tgacacaaca caacgtcaca cctgaagata tgtcagcctt ccgtagttac cagaagaaac
     6421 tcgaactatc agagaccttc cgaaagaatt attccctaga agacgaaatg atctattacc
     6481 aagaccgact agtagtacca ataaaacaac agaacgcagt tatgagacta tatcatgacc
     6541 ataccttatt tggaggacat tttggtgtaa cagtgaccct tgcgaaaatc agcccaattt
     6601 actattggcc aaaattacaa cattcgatca tacaatacat caggacctgc gtacaatgtc
     6661 aactaataaa atcacaccga ccacgcttac atggactatt acaaccactc cctatagcag
     6721 aaggaagatg gcttgatata tcaatggatt ttgtgacagg attacccccg acatcaaata
     6781 acttgaatat gatcctcgtc gtagttgatc gtttttcgaa acgcgctcac ttcatagcta
     6841 caaggaaaac cttagacgca acacaactaa tagatctact ctttcgatac attttttcat
     6901 atcatggttt tcccaggaca ataaccagtg atagagatgt ccgtatgacc gccgacaaat
     6961 atcaagaact cacgaaaaga ctaggaataa aatcgacaat gtcttccgcg aaccaccccc
     7021 aaacagatgg acaatccgaa cgaacgatac agacattaaa caggttacta agagcctatg
     7081 cttcaaccaa tattcagaat tggcatgtat atttaccaca aatcgaattt gtttacaatt
     7141 ctacacctac tagaacactt ggaaaatcac catttgaaat tgatttagga tatttaccga
     7201 atacccctgc tattaagtca gatgacgaag tcaacgcaag aagttttact gccgtagaac
     7261 ttgccaaaca cctcaaagcc cttaccatcc aaacgaagga acagctagaa cacgctcaaa
     7321 tcgaaatgga aactaataac aatcaaagac gtaaaccctt attgttaaac ataggagatc
     7381 acgtattagt gcatagagat gcatacttca agaaaggtgc ttatatgaaa gtacaacaaa
     7441 tatacgtcgg accatttcga gttgtcaaga aaataaacga taacgcctac gaactagatt
     7501 taaactctca caagaaaaag cacagagtta ttaatgtaca attcctgaaa aagtttgtat
     7561 accgtccaga cgcgtaccca aagaataaac caatcagctc cactgaaaga attaagagag
     7621 cacacgaagt tactgcactc ataggaatag atactacaca caaaacttac ttatgtcaca
     7681 tgcaagatgt agacccaaca ctttcagtag aatactcaga agctgaattt tgccaaattc
     7741 ccgaaagaac acgaagatca atattagcca actttagaca actctacgaa acacaagaca
     7801 accctgagag agaggaagat gttgtatctc aaaatgagat atgtcagtat gacaatacgt
     7861 caccctgaac gttcataaaa cacatatgaa acaaccttat aacaaaacga acaacatgag
     7921 acaaaacccg accttcccta gctgaactac ccaaagtata aatgcctgaa caattagttt
     7981 agatccgaga ttccgcgctt ccaccactta gtatgattca tattttatat aatatataag
     8041 ataagtaaca ttccgtgaat taatctgata aactgttttg acaactggtt acttccctaa
     8101 gactgtttat attaggattg tcaagacact ccggtattac tcgagcccgt aatacaacag
     8161 cggccgccac cgcggtggag ctccaattcg ccctatagtg agtcgtatta caattcactg
     8221 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     8281 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     8341 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
     8401 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     8461 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     8521 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     8581 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     8641 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     8701 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8761 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     8821 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     8881 agggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat
     8941 ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc
     9001 ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc
     9061 ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt
     9121 gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt
     9181 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt
     9241 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc
     9301 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct
     9361 gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc
     9421 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag
     9481 caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct
     9541 taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa
     9601 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg
     9661 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac
     9721 taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc
     9781 tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa
     9841 cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct
     9901 gttcggagat taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa
     9961 aaaaaaatga tgaattgaaa aggtggtatg gtgcactctc agtacaatct gctctgatgc
    10021 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
    10081 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
    10141 gaggttttca ccgtcatcac cgaaacgcgc ga
//
