LOCUS       sequence-218746-       10196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of a PDX1:VP16 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10196)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 10196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPG-UBI-PDX1:VP16
FEATURES             Location/Qualifiers
     source          1..10196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..31
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             886..1119
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDTADFEFEQMFTDALGIDEYGG"
     protein_bind    complement(1141..1165)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1182..1692
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1611..1629
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      1708..1717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1714..1716,1717..1719,1720..2433)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1714..1716,1717..1719,1720..2430)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1759..1780)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2020..2039)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2367..2388
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2449..3037
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2502..2522)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2920..2931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3178..3211)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             3231..3411
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3449..3468)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3514..3569
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3568..3587)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3930..3946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3930..3946)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3943..3965)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3954..3970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3978..3984,3985..4002,4003..4008))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4023..4044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4103..4298
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4149..4284
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4304..4438
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4341..4360)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4395..4414
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4506..4523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4677..5264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4757..4776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5435..6226,6227..6295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6058..6077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6296..6400)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6431..6810
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             6812..7069
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7116..7241
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7733..7850
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8210..8443
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8628..8672
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    8947..8967
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        8985..10196
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     10033..10052
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 aggatcccaa ctttgtacaa aaaagcaggc ttcaccatga acagtgagga gcagtactac
       61 gcggccacac agctctacaa ggacccgtgc gcattccaga ggggcccggt gccagagttc
      121 agcgctaacc cccctgcgtg cctgtacatg ggccgccagc ccccacctcc gccgccaccc
      181 cagtttacaa gctcgctggg atcactggag cagggaagtc ctccggacat ctccccatac
      241 gaagtgcccc cgctcgcctc cgacgacccg gctggcgctc acctccacca ccaccttcca
      301 gctcagctcg ggctcgccca tccacctccc ggacctttcc cgaatggaac cgagcctggg
      361 ggcctggaag agcccaaccg cgtccagctc cctttcccgt ggatgaaatc caccaaagct
      421 cacgcgtgga aaggccagtg ggcaggaggt gcttacacag cggaacccga ggaaaacaag
      481 aggacccgta ctgcctacac ccgggcgcag ctgctggagc tggagaagga attcttattt
      541 aacaaataca tctcccggcc ccgccgggtg gagctggcag tgatgttgaa cttgaccgag
      601 agacacatca aaatctggtt ccaaaaccgt cgcatgaagt ggaaaaaaga ggaagataag
      661 aaacgtagta gcgggacccc gagtgggggc ggtgggggcg aagagccgga gcaagattgt
      721 gcggtgacct cgggcgagga gctgctggca gtgccaccgc tgccacctcc cggaggtgcc
      781 gtgcccccag gcgtcccagc tgcagtccgg gagggcctac tgccttcggg ccttagcgtg
      841 tcgccacagc cctccagcat cgatatgacc ggatccctgt cgacggcccc cccgaccgat
      901 gtcagcctgg gggacgagct ccacttagac ggcgaggacg tggcgatggc gcatgccgac
      961 gcgctagacg atttcgatct ggacatgttg ggggacgggg attccccggg gccgggattt
     1021 accccccacg actccgcccc ctacggcgct ctggatacgg ccgacttcga gtttgagcag
     1081 atgtttaccg atgcccttgg aattgacgag tacggtgggc tcgagcctct agaactatag
     1141 acccagcttt cttgtacaaa gtggtgataa tcgaattcca cggggttggg gttgcgcctt
     1201 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
     1261 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
     1321 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
     1381 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact
     1441 agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct
     1501 gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg
     1561 cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca
     1621 ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac
     1681 ctctctcccc agggggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
     1741 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1801 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1861 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1921 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1981 cccgaaggct atgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2041 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2101 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2161 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     2221 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2281 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     2341 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     2401 atcactctcg gcatggacga gctgtacaag taaagcggcc gcgtcgacaa tcaacctctg
     2461 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2521 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2581 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2641 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2701 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     2761 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2821 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     2881 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     2941 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3001 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcccgc ggttcgcttt
     3061 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     3121 actggaaggg ctaattcact cccaacgaag acaagatggg atcaattcac catgggaata
     3181 acttcgtata gcatacatta tacgaagtta tgctgctttt tgcttgtact gggtctctct
     3241 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3301 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3361 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctagt
     3421 tgaatcgcca gtgtgtcgag ctagcagatc tttttccctc tgccaaaaat tatggggaca
     3481 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     3541 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     3601 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     3661 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     3721 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     3781 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     3841 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     3901 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     3961 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     4021 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     4081 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
     4141 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4201 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4261 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
     4321 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4381 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
     4441 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4501 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4561 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4621 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4681 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4741 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4801 cctgttccga ccctgccgct tacggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4861 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4921 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4981 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5041 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5101 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5161 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5221 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5281 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5341 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5401 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5461 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5521 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5581 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5641 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5701 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5761 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5821 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5881 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5941 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6001 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6061 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6121 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6181 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6241 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6301 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6361 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6421 cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     6481 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     6541 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     6601 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     6661 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     6721 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     6781 atctacgtat tagtcatcgc tattaccatg ggaggcgtgg cctgggcggg actggggagt
     6841 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     6901 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6961 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7021 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     7081 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     7141 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     7201 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     7261 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     7321 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatact ggcctgttag
     7381 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     7441 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     7501 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     7561 agaaaaaagc acagcaagca gcaggatctt cagacctgga aattccctac aatccccaaa
     7621 gtcaaggagt agtagaatct atgaataaag aattaaagaa aattatagga caggtaagag
     7681 atcaggctga acatcttaag acagcagtac aaatggcagt attcatccac aattttaaaa
     7741 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     7801 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     7861 acagggacag cagaaatcca ctttggaaag gaccagcaaa gctcctctgg aaaggtgaag
     7921 gggcagtagt aatacaagat aatagtgaca taaaagtagt gccaagaaga aaagcaaaga
     7981 tcattaggga ttatggaaaa cagatggcag gtgatgattg tgtggcaagt agacaggatg
     8041 aggattagaa catggaaaag tttagtaaaa caccataagg aggagatatg agggacaatt
     8101 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     8161 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     8221 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     8281 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     8341 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     8401 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     8461 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     8521 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     8581 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     8641 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     8701 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     8761 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     8821 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     8881 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat ctagtgatta
     8941 tcggatcaac tttgtataga aaagttgtta attaagggtg cagcggcctc cgcgccgggt
     9001 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
     9061 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     9121 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     9181 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     9241 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     9301 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     9361 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctagccgg ggctttcgtg
     9421 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     9481 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
     9541 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
     9601 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
     9661 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     9721 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     9781 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     9841 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     9901 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     9961 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
    10021 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
    10081 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
    10141 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagac
//
