LOCUS       sequence-218747-       14907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Alpha factor inducible Ty3 with his3AI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14907)
  AUTHORS   Bilanchone V, Clemens K, Kaake R, Dawson AR, Matheos D, Nagashima K,
            Sitlani P, Patterson K, Chang I, Huang L, Sandmeyer S
  TITLE     Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to 
            Infect New Genomes.
  JOURNAL   PLoS Genet. 2015 Sep 30;11(9):e1005528. doi: 
            10.1371/journal.pgen.1005528. eCollection 2015.
  PUBMED    26421679
REFERENCE   2  (bases 1 to 14907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2015-09-30"; volume: "11(9):e1005528. doi"; pages: "
            10.1371/journal.pgen.1005528. eCollection 2015"
COMMENT     Sequence Label: pDM3193
FEATURES             Location/Qualifiers
     source          1..14907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(6506..6693)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     rep_origin      8172..9514
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9135..9182)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(9863..9882)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9864..9882)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9903..9919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9903..9919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9916..9938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9927..9943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9951..9957,9958..9975,9976..9981))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9996..10017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10134..10151)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10305..10893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10385..10404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11064..11855,11856..11924))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11687..11706
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11925..12029)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12097..12115
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12153..12175)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12275..12294
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      12661..13174
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12748..12767)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12958..12979
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13712..13734
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13726..13743
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13727..13743
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        13753..13968
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             13969..14772
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 aagcttgtta ttatttaata aaagataaaa gtcacttgta tgaaatgaag cagttatata
       61 tatgttaata agaaaaaggc aaaaacttaa ccctagcaaa aaaggtaatc aatgtgctaa
      121 aacggtaaat atttaaacgc aggttgcgaa ttgccaattg ccaattgtag gatacacagg
      181 gagagtagaa acaaccaagt cctgtgtcct gtggtagacc cacttgtttt accgcagagg
      241 gccctttttt tgtttaccgt cgcaaaaaaa aaacgcgaag aatacgcgtt ggtcgttaaa
      301 ttttgtggta cgcgtaaaac tgctatttgg tatcctcttt agggacgcgc ccattgtcta
      361 tacggtaata tagggctttc ttcattacct gcttgcgtca attagcttta ctcatgtaac
      421 acataataac ctcttacatt gtaatgaggc ccaaaaaaaa aaagaacatc ttcaaaaaaa
      481 aaaactttca tggaaggacc acctagttaa taaaaagctc gcactcagga tcgaactaag
      541 gaccaacaga tttgcaatct gctgcgctac cactgcgcca tacgagcttg attttctgaa
      601 agtgttgtat ctcaaaatga gatatgtcag tatgacaata cgtcaccctg aacgttcata
      661 aaacacatat gaaacaacct tataacaaaa cgaacaacat gagacaaaac ccgaccttcc
      721 ctagctgaac tacccaaagt ataaatgcct gaacaattag tttagatccg agattccgcg
      781 cttccaccac ttagtatgat tcatatttta tataatatat aagataagta acattccgtg
      841 aattaatctg ataaactgtt ttgacaactg gttacttccc taagactgtt tatattagga
      901 ttgtcaagac actccggtat tactcgagcc cgtaatacaa cacctggtag cgttaaaggt
      961 tactaattgt tcaaacgaac catcgaaaag ccgaacctag ctacaccaca ccccagtatg
     1021 agctttatgg atcaaatccc aggaggagga aattatccaa aactcccagt agaatgcctt
     1081 cctaacttcc cgatccaacc atctttgacc ttcagaggta gaaatgactc gcataaactg
     1141 aaaaacttta tctccgaaat aatgttaaac atgtctatga tatcttggcc gaatgatgcc
     1201 agtcgtattg tgtactgcag aagacattta ttaaaccccg ctgctcagtg ggctaatgac
     1261 tttgtacaag aacaaggtat acttgaaata acattcgaca cattcataca aggattatat
     1321 cagcatttct ataagccacc agatatcaat aaaatcttta atgcaatcac gcaactttcc
     1381 gaagctaaac ttggtattga gcgtctcaac caacgattca gaaagatttg ggacagaatg
     1441 ccaccagact tcatgaccga aaaagctgcc ataatgacat atactaggct attgacaaag
     1501 gaaacctata atattgtcag aatgcacaaa ccagagacat taaaagacgc catggaagag
     1561 gcttaccaga caactgcact aactgaaaga ttcttcccag gattcgaact tgatgctgat
     1621 ggagacacta tcatcggtgc cacaacccac ttacaagaag aatacgactc tgactatgat
     1681 tcagaagata atctgaccca gaatggatac gtccataccg taaggacaag aagatcttac
     1741 aataaaccaa tgtcaaatca tcgaaacagg agaaataaca acccatctag agaagaatgt
     1801 ataaaaaatc ggctatgctt ctattgtaag aaagagggac atcgcctgaa cgaatgtaga
     1861 gcacgtaagg cgagttctaa ccgatcttga actcgaatca aaagaccaac aaactccttt
     1921 tatcaaaacc ttaccaattg tacactatat cgccatcccc gagatggaca ataccgccga
     1981 aaaaaccata aaaatacaaa acacgaaagt aaaaaccctg tttgacagtg gatcacccac
     2041 gtcatttatc cgaagagata ttgtagaact tctcaaatac gaaatctacg agacccctcc
     2101 actccgtttt agaggattcg tagccaccaa atccgccgtt acatccgaag cagtcaccat
     2161 tgacctcaaa atcaatgacc tgcatataac tttagccgcg tacatactgg ataacatgga
     2221 ctaccaattg ttaattggaa atccaatctt acgccgctac ccgaaaatcc tgcacacagt
     2281 actgaatacc agagagagcc ccgactcctt aaagcccaag acttatcgct ccgaaaccgt
     2341 taataacgtt agaacctact ccgctggtaa tcgtggtaac cccagaaaca taaaactgtc
     2401 ttttgccccc accattctcg aagcaactga cccgaaatcc gctggtaatc gtggtgactc
     2461 cagaaccaaa accctgtctc ttgcaaccac tactcctgca gcaattgacc cgcttacgac
     2521 ccttgataac ccaggtagta ctcaaagtac atttgcgcaa ttcccgatac ctgaagaagc
     2581 gagcatccta gaagaggatg gaaaatactc caacgttgtc tcaaccattc agagtgtaga
     2641 acctaatgct actgatcaca gcaataagga caccttttgc actttgccag tttggttaca
     2701 acagaagtat agagagatca tacgtaatga tctcccacca agacctgccg acattaataa
     2761 catccccgta aaacatgata ttgaaattaa acctggcgca agactacctc gactacagcc
     2821 ataccatgtt acagaaaaga acgaacaaga aatcaacaaa atagttcaaa aactgctcga
     2881 taacaagttc attgttccct caaagtcgcc ttgcagctcc cctgtagtcc tcgtcccgaa
     2941 gaaagacggt accttccgac tctgcgtcga ttaccgcacc ctgaacaaag ctaccatctc
     3001 cgacccattc ccattaccca gaatcgacaa cctattgagc cgtattggaa atgcccagat
     3061 atttaccacg ctagatttgc atagtggtta ccaccagatc ccgatggaac ccaaagaccg
     3121 ctacaaaacc gcctttgtca caccatccgg taagtatgaa tataccgtca tgccatttgg
     3181 cttagtcaat gcacctagta cattcgcaag atacatggct gatacattta gagacctgag
     3241 attcgtcaat gtttaccttg atgatatatt aatattctcc gaatctccag aagaacattg
     3301 gaaacattta gacacggtac tagaaagatt aaagaacgag aacctcattg ttaagaagaa
     3361 aaaatgtaaa tttgcatctg aagaaactga gtttttaggc tatagtattg gaatccagaa
     3421 aatagctcca ctacagcaca aatgtgcagc aatccgagac tttccgacgc ctaaaacagt
     3481 aaaacaagca cagagatttt taggaatgat taattactac agacgattca ttccaaattg
     3541 ctccaagatt gcacagccaa tccaactgtt tatttgtgac aaaagtcaat ggacagaaaa
     3601 acaagacaag gcaattgata aactaaaaga cgccttgtgt aactcccccg tcctagtacc
     3661 attcaacaac aaagcaaact accgacttac aacagacgcc tcaaaagacg gcattggtgc
     3721 tgttctagaa gaagtcgaca acaagaacaa acttgttggt gtcgtcggtt acttctctaa
     3781 atccttagag agtgcccaga aaaactatcc tgctggcgaa ttagaactac ttggaattat
     3841 caaagcactc caccacttcc gatatatgct tcacggaaag catttcacgt taagaacaga
     3901 ccacattagt ttgttatcat tacaaaacaa gaacgaaccc gcacgacgcg tgcaacgctg
     3961 gttagatgac ctagccacat atgacttcac cttagaatac ctagctggac ccaagaacgt
     4021 tgtcgcagat gccatatccc gtgccgtata tactataacc cccgaaacat cccgacctat
     4081 cgacacagaa agctggaaat cttactacaa atcagaccca ttatgtagtg ctgtcttaat
     4141 tcatatgaaa gaattgacac aacacaacgt cacacctgaa gatatgtcag ccttccgtag
     4201 ttaccagaag aaactcgaac tatcagagac cttccgaaag aattattccc tagaagacga
     4261 aatgatctat taccaagacc gactagtagt accaataaaa caacagaacg cagttatgag
     4321 actatatcat gaccatacct tatttggagg acattttggt gtaacagtga cccttgcgaa
     4381 aatcagccca atttactatt ggccaaaatt acaacattcg atcatacaat acatcaggac
     4441 ctgcgtacaa tgtcaactaa taaaatcaca ccgaccacgc ttacatggac tattacaacc
     4501 actccctata gcagaaggaa gatggcttga tatatcaatg gattttgtga caggattacc
     4561 cccgacatca aataacttga atatgatcct cgtcgtagtt gatcgttttt cgaaacgcgc
     4621 tcacttcata gctacaagga aaaccttaga cgcaacacaa ctaatagatc tactctttcg
     4681 atacattttt tcatatcatg gttttcccag gacaataacc agtgatagag atgtccgtat
     4741 gaccgccgac aaatatcaag aactcacgaa aagactagga ataaaatcga caatgtcttc
     4801 cgcgaaccac ccccaaacag atggacaatc cgaacgaacg atacagacat taaacaggtt
     4861 actaagagcc tatgcttcaa ccaatattca gaattggcat gtatatttac cacaaatcga
     4921 atttgtttac aattctacac ctactagaac acttggaaaa tcaccatttg aaattgattt
     4981 aggatattta ccgaataccc ctgctattaa gtcagatgac gaagtcaacg caagaagttt
     5041 tactgccgta gaacttgcca aacacctcaa agcccttacc atccaaacga aggaacagct
     5101 agaacacgct caaatcgaaa tggaaactaa taacaatcaa agacgtaaac ccttattgtt
     5161 aaacatagga gatcacgtat tagtgcatag agatgcatac ttcaagaaag gtgcttatat
     5221 gaaagtacaa caaatatacg tcggaccatt tcgagttgtc aagaaaataa acgataacgc
     5281 ctacgaacta gatttaaact ctcacaagaa aaagcacaga gttattaatg tacaattcct
     5341 gaaaaagttt gtataccgtc cagacgcgta cccaaagaat aaaccaatca gctccactga
     5401 aagaattaag agagcacacg aagttactgc actcatagga atagatacta cacacaaaac
     5461 ttacttatgt cacatgcaag atgtagaccc aacactttca gtagaatact cagaagctga
     5521 attttgccaa attcccgaaa gaacacgaag atcaatatta gccaacttta gacaactcta
     5581 cgaaacacaa gacaaccctg agagagagga agatgttgta tctcaaaatg agatatgtca
     5641 gtatgacaat acgtcaccct gaacgttcat aaaacacata tgaaacaacc ttataacaaa
     5701 tcgataagct tggctgcagc tttaaataat cggtgtcact acataagaac acctttggtg
     5761 gagggaacat cgttggtacc attgggcgag gtggcttctc ttatggcaac cgcaagagcc
     5821 ttgaacgcac tctcactacg gtgatgatca ttcttgcctc gcagacaatc aacgtggagg
     5881 gtaattctgc tagcctctgc aaagctttca agaaaatgcg ggatcatctc gcaagagaga
     5941 tctcctactt tctccctttg caaaccaagt tcgacaactg cgtacggcct gttcgaaaga
     6001 tctaccaccg ctctggaaag tgcctcatcc aaaggcgcaa atcctgatcc aaaccttttt
     6061 actccacgca cggcccctag ggcctcttta aaagcttgac cgagagcaat cccgcagtct
     6121 tcagtggtgt gatggtcgtc tatgtgtaag tcaccaatgc actcaacgat tagcgaccag
     6181 ccggaatgct tgggtatgtt aatatggact aaaggaggct tttctgcagg tcgactctag
     6241 aggatccccg ggtaccgagc tcgaattttt actaacaaat ggtattattt ataacagcca
     6301 gagcatgtat catatggtcc agaaacccta tacctgtgtg gacgttaatc acttgcgatt
     6361 gtgtggcctg ttctgctact gcttctgcct ctttttctgg gaagatcgag tgctctatcg
     6421 ctaggggacc accctttaaa gagatcgcaa tctgaatctt ggtttcattt gtaatacgct
     6481 ttactagggc tttctgctct gtcatctttg ccttcgttta tcttgcctgc tcatttttta
     6541 gtatattctt cgaagaaatc acattacttt atataatgta taattcatta tgtgataatg
     6601 ccaatcgcta agaaaaaaaa agagtcatcc gctaggtgga aaaaaaaaaa tgaaaatcat
     6661 taccgaggca taaaaaaata tagagtgtac tagaggctcc aagagtgtac tagaggatcc
     6721 ccgggcgagc tcgaattccg agcttatcga tcaacatgag acaaaacccg accttcccta
     6781 gctgaactac ccaaagtata aatgcctgaa caattagttt agatccgaga ttccgcgctt
     6841 ccaccactta gtatgattca tattttatat aatatataag ataagtaaca ttccgtgaat
     6901 taatctgata aactgttttg acaactggtt acttccctaa gactgtttat attaggattg
     6961 tcaagacact ccggtattac tcgagcccgt aatacaacag aaagttccat tttggatgct
     7021 ctatttatgg gaatatgact atacgaatgt cacccacgat ggccggagta gctgtgatga
     7081 gcatcttttt gtcctgggcg gcttaaaacg tgagcaaaat tgtactgtaa attacttacg
     7141 ttatcaatgg aagtgtgtga atttcgtgat aaaattattg tcagaatagt gacttcatta
     7201 ttctttataa ctaaccatta ggatcatata agtaatcaga tgcagttcgt cattaatatg
     7261 tcaggatgga taaccatagg taagcacgtg attatggaag gacagtaaag ttaccgatct
     7321 acttttttga atgatcaact tggtgttttc atctataagg atatgggtcg ttacattgaa
     7381 actatagtaa aagatctcgt tattggtata tcggtcataa tgaagatgaa atagtcggta
     7441 tgtattttta tacccccccc tatatggtaa aaaggttaag attcatattt aaatcttcat
     7501 taataccacc tctaatttct aattaccaac atttttaact ccacttttat tatatgctat
     7561 atacaacatt atgtaaaagg agtataccaa aaattctcct tcataatata ggaattccac
     7621 ggactataga ctatactagt atactccgtc tactgtacga tacacttccg ctcaggtcct
     7681 tgtcctttaa cgaggcctta ccactctttt gttactctat tgatccagct cagcaaaggc
     7741 agtgtgatct aagattctat cttcgcgatg tagtaaaact agctagaccg agaaagagac
     7801 tagaaatgca aaaggcactt ctacaatggc tgccatcatt attatccgat gtgacgctgc
     7861 agcttctcaa tgatattcga atacgctttg aggagataca gcctaatatc cgacaaactg
     7921 ttttacagat ttacgatcgt acttgttacc catcattgaa ttttgaacat ccgaacctgg
     7981 gagttttccc tgaaacagat agtatatttg aacctgtata ataatatata gtctagcgct
     8041 ttacggaaga caatgtatgt atttcggttc ctggagaaac tattgcatct attgcatagg
     8101 taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca cttcaatagc
     8161 atatctttgt taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag
     8221 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     8281 aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc
     8341 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     8401 cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc
     8461 atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc
     8521 gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa
     8581 ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt
     8641 actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat
     8701 tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct
     8761 tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg
     8821 aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt
     8881 ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg
     8941 caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata
     9001 tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc
     9061 tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt
     9121 ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact
     9181 tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc
     9241 tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga
     9301 acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg
     9361 aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc
     9421 cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat
     9481 ccttcaatgc tatcatttcc tttgatattg gatcatatgc atagtaccga gaaactagtg
     9541 cgaagtagtg atcaggtatt gctgttatct gatgagtata cgttgtcctg gccacggcag
     9601 aagcacgctt atcgctccaa tttcccacaa cattagtcaa ctccgttagg cccttcattg
     9661 aaagaaatga ggtcatcaaa tgtcttccaa tgtgagattt tgggccattt tttatagcaa
     9721 agattgaata aggcgcattt ttcttcaaag ctttattgta cgatctgact aagttatctt
     9781 ttaataattg gtattcctgt ttattgcttg aagaattgcc ggtcctattt actcgtttta
     9841 ggactggttc agaattcggt ctccctatag tgagtcgtat taattcgata tccgtaatca
     9901 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9961 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    10021 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    10081 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    10141 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    10201 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    10261 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    10321 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    10381 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10441 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10501 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10561 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    10621 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10681 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10741 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10801 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10861 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10921 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10981 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    11041 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    11101 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    11161 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    11221 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    11281 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    11341 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    11401 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    11461 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    11521 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    11581 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    11641 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    11701 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    11761 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    11821 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11881 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11941 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    12001 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    12061 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    12121 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    12181 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    12241 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
    12301 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
    12361 cgattccgtt gcaatggctg gcggtaatat tgttctggat attaccagca aggccgatag
    12421 tttgagttct tctactcagg caagtgatgt tattactaat caaagaagta ttgcgacaac
    12481 ggttaatttg cgtgatggac agactctttt actcggtggc ctcactgatt ataaaaacac
    12541 ttctcaggat tctggcgtac cgttcctgtc taaaatccct ttaatcggcc tcctgtttag
    12601 ctcccgctct gattctaacg aggaaagcac gttatacgtg ctcgtcaaag caaccatagt
    12661 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    12721 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    12781 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    12841 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    12901 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    12961 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
    13021 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
    13081 acgcgaattt taacaaaata ttaacgctta caatttaaat atttgcttat acaatcttcc
    13141 tgtttttggg gcttttctga ttatcaaccg gggtacatat gattgacatg ctagttttac
    13201 gattaccgtt catcgattct cttgtttgct ccagactctc aggcaatgac ctgatagcct
    13261 ttgtagacct ctcaaaaata gctaccctct ccggcatgaa tttatcagct agaacggttg
    13321 aatatcatat tgatggtgat ttgactgtct ccggcctttc tcacccgttt gaatctttac
    13381 ctacacatta ctcaggcatt gcatttaaaa tatatgaggg ttctaaaaat ttttatcctt
    13441 gcgttgaaat aaaggcttct cccgcaaaag tattacaggg tcataatgtt tttggtacaa
    13501 ccgatttagc tttatgctct gaggctttat tgcttaattt tgctaattct ttgccttgcc
    13561 tgtatgattt attggatgtt ggaacgccat tcgccattca ggctgcgcaa ctgttgggaa
    13621 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
    13681 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
    13741 agtgccaagc ttttcaattc atcatttttt ttttattctt ttttttgatt tcggtttcct
    13801 tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga
    13861 cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc
    13921 ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct
    13981 acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc
    14041 atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta
    14101 ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc
    14161 ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac
    14221 aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag
    14281 tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg
    14341 gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct
    14401 agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat
    14461 actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct
    14521 caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg
    14581 ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc
    14641 tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct
    14701 aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc
    14761 cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga
    14821 gcttcaattt aattatatca gttattaccc gggaatctcg gtcgtaatga tttttataat
    14881 gacgaaaaaa aaaaaattgg aaagaaa
//
