LOCUS       sequence-218748-       10756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of Wnt3a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De 
            Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ
  TITLE     Perturbed Wnt signaling leads to neuronal migration delay, altered 
            interhemispheric connections and impaired social behavior.
  JOURNAL   Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w.
  PUBMED    29079819
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-27"; volume: "8(1):1158. doi"; pages: " 
            10.1038/s41467-017-01046-w"
COMMENT     Sequence Label: pCWXPGR-pTF-Wnt3a
FEATURES             Location/Qualifiers
     source          1..10756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    81..105
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1170..1194)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1211..1721
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1640..1658
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      1737..1746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1743..1745,1746..1748,1749..2459)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1743..1745,1746..1748,1749..2459)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1788..1809)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2049..2068)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2396..2417
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2475..2534
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             3603..3974
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    4000..4588
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4053..4073)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4471..4482)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4591..4607)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4592..4608)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(4736..4769)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4789..4969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5007..5026)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5072..5127
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5126..5145)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5488..5504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5488..5504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5501..5523)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5512..5528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5536..5542,5543..5560,5561..5566))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5581..5602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5661..5856
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5707..5842
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5862..5996
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5899..5918)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5953..5972
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6064..6081)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6235..6822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6315..6334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6993..7784,7785..7853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7616..7635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7854..7958)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7989..8368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8370..8627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8674..8799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9291..9408
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9768..10001
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10186..10230
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    10505..10525
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    10552..10570
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    10593..10611
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 actctatcat tgatagagtt ctagtattag caaggccttt cctctatata aggaagttca
       61 tttcatttgg agaagatccc ccaagtttgt acaaaaaagc aggcttcacc atggctcctc
      121 tcggatacct cttagtgctc tgcagcctga agcaggctct gggcagctac ccgatctggt
      181 ggtccttggc tgtgggaccc cagtactcct ctctgagcac tcagcccatt ctctgtgcca
      241 gcatcccagg cctggtaccg aagcagctgc gcttctgcag gaactacgtg gagatcatgc
      301 ccagcgtggc tgagggtgtc aaagcgggca tccaggagtg ccagcaccag ttccgaggcc
      361 ggcgttggaa ctgcaccacc gtcagcaaca gcctggccat ctttggccct gttctggaca
      421 aagccacccg ggagtcagcc tttgtccatg ccatcgcctc cgctggagta gctttcgcag
      481 tgacacgctc ctgtgcagag ggatcagctg ctatctgtgg gtgcagcagc cgcctccagg
      541 gctccccagg cgagggctgg aagtggggcg gctgtagtga ggacattgaa tttggaggaa
      601 tggtctctcg ggagtttgcc gatgccaggg agaaccggcc ggatgcccgc tctgccatga
      661 accgtcacaa caatgaggct gggcgccagg ccatcgccag tcacatgcac ctcaagtgca
      721 aatgccacgg gctatctggc agctgtgaag tgaagacctg ctggtggtcg cagccggact
      781 tccgcaccat cggggatttc ctcaaggaca agtatgacag tgcctcggag atggtggtag
      841 agaaacaccg agagtctcgt ggctgggtgg agaccctgag gccacgttac acgtacttca
      901 aggtgccgac agaacgcgac ctggtctact acgaggcctc acccaacttc tgcgaaccta
      961 accccgaaac cggctccttc gggacgcgtg accgcacctg caatgtgagc tcgcatggca
     1021 tagatgggtg cgacctgttg tgctgcgggc gcgggcataa cgcgcgcact gagcgacgga
     1081 gggagaaatg ccactgtgtt ttccattggt gctgctacgt cagctgccag gagtgcacac
     1141 gtgtctatga cgtgcacacc tgcaagtaga cccagctttc ttgtacaaag tggtgataat
     1201 cgaattccac ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
     1261 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
     1321 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
     1381 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
     1441 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag
     1501 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg
     1561 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg
     1621 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag
     1681 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca ccggtcgcca
     1741 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1801 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     1861 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     1921 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     1981 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2041 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2101 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2161 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2221 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2281 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2341 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2401 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     2461 ctagcggatc aggtcaatgt actaactacg ctttgttgaa gcttgccggc gatgttgaaa
     2521 gtaaccccgg tcctggatcc atgtccagac tggacaagag caaagtcata aacggcgctc
     2581 tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
     2641 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
     2701 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt
     2761 catggcaaga ctttctgcgg aacaacgcca agtcattccg ctgtgctctc ctctcacatc
     2821 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
     2881 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
     2941 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
     3001 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa
     3061 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
     3121 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccggcctcg agcgaattcc
     3181 agtacctgcc agatacagac gatcgtcacc ggattgagga gaaacgtaaa aggacatatg
     3241 agaccttcaa gagcatcatg aagaagagtc ctttcagcgg acccaccgac ccccggcctc
     3301 cacctcgacg cattgctgtg ccttcccgca gctcagcttc tgtccccaag ccagcacccc
     3361 agccctatcc ctttacgtca tccctgagca ccatcaacta tgatgagttt cccaccatgg
     3421 tgtttccttc tgggcagatc agccaggcct cggccttggc cccggcccct ccccaagtcc
     3481 tgccccaggc tccagcccct gcccctgctc cagccatggt atcagctctg gcccaggccc
     3541 cagcccctgt cccagtccta gccccaggcc ctcctcaggc tgtggcccca cctgccccca
     3601 agcccaccca ggctggggaa ggaacgctgt cagaggccct gctgcagctg cagtttgatg
     3661 atgaagacct gggggccttg cttggcaaca gcacagaccc agctgtgttc acagacctgg
     3721 catccgtcga caactccgag tttcagcagc tgctgaacca gggcatacct gtggcccccc
     3781 acacaactga gcccatgctg atggagtacc ctgaggctat aactcgccta gtgacagggg
     3841 cccagaggcc ccccgaccca gctcctgctc cactgggggc cccggggctc cccaatggcc
     3901 tcctttcagg agatgaagac ttctcctcca ttgcggacat ggacttctca gccctgctga
     3961 gtcagatcag ctcctaaaga tctgatcaag cttatcgata atcaacctct ggattacaaa
     4021 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4081 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4141 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4201 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4261 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4321 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4381 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4441 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4501 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4561 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg ttcgctttaa
     4621 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4681 tggaagggct aattcactcc caacgaagac aagatgggat caattcacca tgggaataac
     4741 ttcgtatagc atacattata cgaagttatg ctgctttttg cttgtactgg gtctctctgg
     4801 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4861 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     4921 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagttg
     4981 aatcgccagt gtgtcgagct agcagatctt tttccctctg ccaaaaatta tggggacatc
     5041 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     5101 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     5161 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     5221 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     5281 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     5341 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     5401 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     5461 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5521 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5581 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5641 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5701 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5761 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5821 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt
     5881 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5941 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
     6001 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6061 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6361 tgttccgacc ctgccgctta cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7981 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     8041 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     8101 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     8161 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     8221 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     8281 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     8341 ctacgtatta gtcatcgcta ttaccatggg aggygtggcc tgggcgggac tggggagtgg
     8401 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     8461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8641 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     8701 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8761 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8821 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8881 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatactgg cctgttagaa
     8941 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     9001 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     9061 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     9121 aaaaaagcac agcaagcagc aggatcttca gacctggaaa ttccctacaa tccccaaagt
     9181 caaggagtag tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat
     9241 caggctgaac atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga
     9301 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     9361 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     9421 agggacagca gaaatccact ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg
     9481 gcagtagtaa tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc
     9541 attagggatt atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag
     9601 gattagaaca tggaaaagtt tagtaaaaca ccataaggag gagatatgag ggacaattgg
     9661 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     9721 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     9781 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     9841 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     9901 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     9961 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    10021 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    10081 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    10141 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    10201 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    10261 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    10321 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    10381 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    10441 agagacagag acagatccat tcgattagtg aacggatctc gacggtatct agtgattatc
    10501 ggatcaactt tgtatagaaa agttgggtac aactcccact agacatatcc tactctatca
    10561 ttgatagagt gacccttcca tagacatatc ctactctatc attgatagag tgacccttcc
    10621 actaggttca cttttctcta tcattgatag ggagtggtaa acaattgatg gacaaaatct
    10681 atcaatgata gagagtgcat gttactagta ttgttcttct ctatcattga tagtgacttt
    10741 gcctaatcac ttagca
//
