LOCUS       sequence-218752-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express gfp driven by SFFV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: pscALPS gfp Blasti
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(13..15,16..18,19..729)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 tctagagccg ccatggtctc aaagggggaa gagctgttta ctggggtcgt gcctattctg
       61 gtcgaactgg acggggatgt caacggccac aagtttagtg tgagcggaga gggagaaggc
      121 gacgccactt acggaaagct gaccctgaaa ttcatttgca ccacaggcaa gctgccagtg
      181 ccctggccta ccctggtcac taccctgaca tacggcgtcc agtgttttag taggtatcct
      241 gaccacatga agcagcatga tttctttaaa tcagctatgc cagagggata cgtgcaggaa
      301 cgcaccattt tctttaagga cgatggcaac tacaagactc gagcagaggt gaagttcgaa
      361 ggggacaccc tggtcaaccg gatcgagctg aagggaatcg actttaaaga agatggaaac
      421 atcctgggcc acaagctgga gtacaactat aattctcata acgtgtacat catggccgat
      481 aagcagaaaa acgggattaa ggtcaatttc aaaatcagac acaacatcga agacggcagc
      541 gtgcagctgg ctgatcatta ccagcagaac acaccaattg gcgacgggcc agtgctgctg
      601 ccagataatc actatctgag cactcagtcc gccctgtcta aggaccctaa cgagaaacgc
      661 gatcatatgg tgctgctgga gttcgtcaca gccgctggga tcactctggg aatggacgag
      721 ctgtataaat gagaattc
//
