LOCUS       sequence-218753-        2256 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express gag-gfp fusion driven by EIF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: pecALPS gag-gfp Blasti
FEATURES             Location/Qualifiers
     source          1..2256
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1531..1533,1534..1536,1537..2247)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 tctagagccg ccatggcggc aagggcaagc gtgctgagcg ggggggagct ggacaaatgg
       61 gagaaaatcc ggctgagacc tggaggcaag aaacagtata aactgaagca catcgtgtgg
      121 gctagcagag aactggagag gttcgcagtc aaccccggcc tgctggaaac atccgaggga
      181 tgccgacaga ttctggggca gctgcagcct tccctgcaga ctggatccga ggaactgcgc
      241 agtctgtaca acacagtggc caccctgtat tgcgtgcatc agcggattga cgtgaaagat
      301 accaaggagg ctctggacaa aatcgaggaa gagcagaata agtccaagaa aaaggcacag
      361 caggcggccg cagatacagg gaacaatagc caggtgtccc agaactaccc aatcgtccag
      421 aatctgcagg gacagatggt gcaccaggcc atttctccca gaaccctgaa cgcttgggtg
      481 aaagtggtcg aagagaaggc cttcagtcct gaagtcatcc caatgttttc tgctctgagt
      541 gagggcgcaa cacctcaaga tctaaacaca atgctgaata ctgtgggcgg gcatcaggcc
      601 gctatgcaga tgctgaagga aactatcaat gaagaggcag cagagtggga caggctgcac
      661 ccagtgcatg caggaccaat tgcacctggg cagatgcgag aacctagagg atcagatatc
      721 gccggcacca caagcacact gcaggagcag attgggtgga tgactcacaa cccccctata
      781 ccggtaggag aaatctacaa aaggtggatc attctgggcc tgaataagat cgtccgcatg
      841 tattcaccca ctagcatcct ggacattcga caaggcccaa aagagccctt ccgcgactac
      901 gtggacagat tctacaagac cctgcgcgcc gaacaagctt cacaggaggt gaaaaactgg
      961 atgactgaga ccctgctggt ccagaacgct aatcctgatt gcaaaacaat tctgaaggcc
     1021 ctggggccag gagctactct ggaagagatg atgaccgcat gtcagggagt gggaggacct
     1081 ggacacaagg ccagagtgct ggctgaggca atgagccagg tcaccaatcc agccacaatc
     1141 atgattcaga agggcaactt ccggaaccag cgcaaaaccg tgaagtgctt taactgtggc
     1201 aaagaggggc atatcgcaaa gaattgcagg gcccctcgga aaaagggatg ctggaaatgt
     1261 ggaaaggaag gccaccagat gaaggactgt actgagagac aggctaactt cctgggcaaa
     1321 atttggccct cccataaggg gagacccggg aattttctgc agagccggcc tgagccaacc
     1381 gcaccacccg aagagagttt caggtttggc gaagagacta ccacaccatc acagaaacag
     1441 gaacccatcg acaaggagct gtatcctctg gcaagtctgc gaagcctgtt tggaagcgac
     1501 ccctcatctc agggcggaag cggcggatcc atggtctcaa agggggaaga gctgtttact
     1561 ggggtcgtgc ctattctggt cgaactggac ggggatgtca acggccacaa gtttagtgtg
     1621 agcggagagg gagaaggcga cgccacttac ggaaagctga ccctgaaatt catttgcacc
     1681 acaggcaagc tgccagtgcc ctggcctacc ctggtcacta ccctgacata cggcgtccag
     1741 tgttttagta ggtatcctga ccacatgaag cagcatgatt tctttaaatc agctatgcca
     1801 gagggatacg tgcaggaacg caccattttc tttaaggacg atggcaacta caagactcga
     1861 gcagaggtga agttcgaagg ggacaccctg gtcaaccgga tcgagctgaa gggaatcgac
     1921 tttaaagaag atggaaacat cctgggccac aagctggagt acaactataa ttctcataac
     1981 gtgtacatca tggccgataa gcagaaaaac gggattaagg tcaatttcaa aatcagacac
     2041 aacatcgaag acggcagcgt gcagctggct gatcattacc agcagaacac accaattggc
     2101 gacgggccag tgctgctgcc agataatcac tatctgagca ctcagtccgc cctgtctaag
     2161 gaccctaacg agaaacgcga tcatatggtg ctgctggagt tcgtcacagc cgctgggatc
     2221 actctgggaa tggacgagct gtataaatga gaattc
//
