LOCUS       sequence-218772-         462 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized 3xFLAG-SIVDEB Vpr (FJ919724) and puromycin 
            resistance protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: pscALPS-SIV-DEB vpr
FEATURES             Location/Qualifiers
     source          1..462
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..87
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 tctagagccg ccatggatta taaagatgac gatgacaaag actataaaga cgatgacgac
       61 aaagattaca aggacgacga cgacaaagcg gccgctatgg agcggcctcc accctctcac
      121 ccacttacgc cgctttccag actcgtcccg attagtaggc tccaacttca atacatgatg
      181 caggatgtga acgaggaagc catgaagcat tttcaaaggg acgaactcat tggcgtatgg
      241 aattattgcg tagaactgcc tgctgaacca gggtggacgg gagaacaagc atgggcggcc
      301 tccgttattg attacattaa aaaggttcag aagatgattt ggctccacct cgccgagggt
      361 tgctatcatc ggagagcagc cgcgagccga cattacccta atataagacc tcttagtgga
      421 cggaaccaag aagtccgcga ccaagaataa cccggggaat tc
//
