LOCUS       sequence-218774-         468 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and 
            Puro resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: pscALPS-SIV-AGM-VER9063 vpr
FEATURES             Location/Qualifiers
     source          1..468
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..87
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 tctagagccg ccatggatta taaagatgac gatgacaaag actataaaga cgatgacgac
       61 aaagattaca aggacgacga cgacaaagcg gccgctatgg cttctggacg gggtcccagg
      121 gaaaaccgcc caggcgaggt tgaaatttgg gatctgaatc gggaaccatg ggacgagtgg
      181 cttcgagaca tgctggagga ccttaatcaa gaagccaaac ttcactttgg tcgagaactg
      241 ctttttcagg tctggaacta ttgccaggaa gaaggcgaga ggcggggggc gcctatgacg
      301 gaacgcgcct atcggtatta ccgcctggtg caaaaggcac tctttgtgca tttccgatgt
      361 ggatgcagga gaaggcaacc atttgagcca tatgaagaga gaagagacgg gcaaggtgga
      421 ggaagggcta acagagctcc tccgggactc gactaacccg gggaattc
//
