LOCUS       sequence-218777-         474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized 3xFLAG-SIVAGM Vpr (MAL_ZMB) (LC114462) and 
            puromycin resistance protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: pscALPS-SIV-AGM-MAL vpr
FEATURES             Location/Qualifiers
     source          1..474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..87
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 tctagagccg ccatggatta taaagatgac gatgacaaag actataaaga cgatgacgac
       61 aaagattaca aggacgacga cgacaaagcg gccgctatgg catccggggg ctccgagaac
      121 cgagaacgca gaccgggatg gcttgagatc tgggatttgt cacgagagcc ttgggacgaa
      181 tggcttaggg acatgcttac tgacctcaac caagaggcaa ggaggcactt cggtcgagag
      241 ttgctgtttc aagtctggaa ttattgtcag gaggaaggtg agaggaatga ggtgcccatg
      301 agtgaacggg cctaccggta ttaccggatt gtgcagaagg ctctctttgt ccattttcga
      361 tgcggttgtc gacgacgaac accatttgag ccctatgaag aaaggcgcaa tggagagggt
      421 ggcggcagag ccggcagagt tcctccggga ttggaagact aacccgggga attc
//
