LOCUS       sequence-218787-         411 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized 3xFLAG-SIVRCM Vpr (HM803689) and puromycin 
            resistance protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: pscALPS-SIV-RCM-vpr
FEATURES             Location/Qualifiers
     source          1..411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..87
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 tctagagccg ccatggatta taaagatgac gatgacaaag actataaaga cgatgacgac
       61 aaagattaca aggacgacga cgacaaagcg gccgctatgg agatgcctcc cgaggatgag
      121 gggcctcagc gcgaaccaat gaatgaatgg cttcttgata cgcttatgga gttgcaagag
      181 gaagcggcta agcactttac tatggagttg ctgcacgcgg taggtaatta tatatatgag
      241 caatatggtg atagcctcga aggcgtccgc gtcctcatca cattgcttca gcgagccctt
      301 ttcctgcact acaggcacgg ttgcacgggt agtaggatag gaacgaatcg aggaggtaat
      361 cccttgcaat caattccgct ctctaggaac atattgtaac ccggggaatt c
//
