LOCUS       sequence-218789-         393 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized 3xFLAG-SIVCOLVpr (KF214240) and puromycin 
            resistance protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: pscALPS-SIV-COL-BWC01 vpr
FEATURES             Location/Qualifiers
     source          1..393
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..87
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 tctagagccg ccatggatta taaagatgac gatgacaaag actataaaga cgatgacgac
       61 aaagattaca aggacgacga cgacaaagcg gccgctatgt ccgaacaagc gccaaaacgc
      121 cctcgggaag gggttcctga ggacgaaacc gttgaacgcg acttcaattg gctccaagaa
      181 caagttgagc tgattaccca agaggctcgc agacactttc ctttgactca tatcagcgta
      241 ataatgatgg ggtgtgtacg gaggcattgg cctgatcaat gggccgcagc tattgcctat
      301 gtaagaatac tggaatccgc tctctatctt cacattcacg ccggccagtg taagttccca
      361 tcaaggacaa acgctccata acccggggaa ttc
//
