LOCUS       sequence-21880-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: FLAG-GAS41
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcatgc cagactacaa ggacgatgat gacaagctcg
      121 agatgttcaa gagaatggcg gaatttggac ctgactccgg cgggagagtg aagggggtta
      181 ccatcgttaa gccaatcgtt tatggcaatg ttgccaggta ctttggaaag aagagagaag
      241 aagacgggca cactcaccag tggactgtgt acgtgaagcc ctacagaaac gaggatatgt
      301 cagcatatgt gaagaagatc cagtttaaat tacacgaaag ctacggcaat cctctaagag
      361 tcgtcaccaa gcccccatat gaaatcacag aaacaggatg gggtgaattt gaaatcatca
      421 tcaagatatt tttcattgac cccaacgaga gacctgtgac tctgtaccac ttactgaagc
      481 tcttccagtc tgacaccaat gccatgctgg ggaagaaaac agtggtttca gagttctatg
      541 acgaaatgat atttcaagac ccaacggcaa tgatgcagca gctgcttacg acgtctcgcc
      601 agctgacatt gggagcctat aagcacgaaa cagagtttgc agaacttgaa gtgaaaacca
      661 gagaaaaatt agaagctgcc aagaaaaaaa caagctttga gattgcggag cttaaggaga
      721 gattaaaagc aagtcgtgaa actataaact gtttaaaaaa tg
//
