LOCUS       sequence-218808-       10158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site 
            of FlpIn cells upon addition of doxycyclin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Vleugel M, Hoek TA, Tromer E, Sliedrecht T, Groenewold V, Omerzu M, 
            Kops GJ
  TITLE     Dissecting the roles of human BUB1 in the spindle assembly 
            checkpoint.
  JOURNAL   J Cell Sci. 2015 Aug 15;128(16):2975-82. doi: 10.1242/jcs.169821. 
            Epub 2015 Jul 6.
  PUBMED    26148513
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.169821"; journalName: "J Cell Sci"; date: "2015-08-15- 
            15"; volume: "128"; issue: "16"; pages: "2975-82"
COMMENT     Sequence Label: pcDNA5-MIS12-LAP-BUB1
FEATURES             Location/Qualifiers
     source          1..10158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1018..1027
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1024..1026,1027..1029,1030..1740)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1069..1090)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1330..1349)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1677..1698
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1786..1806
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1819..1863
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(6110..6127)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6116..6340
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6473..6492)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6624..6671
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6679..7701
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6679..7701
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7831..7952
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7868..7887)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7922..7941
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8001..8017)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8001..8017)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8014..8036)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8025..8041
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8049..8055,8056..8073,8074..8079))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8094..8115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8232..8249)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8403..8991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8483..8502)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9162..9953,9954..10022))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9785..9804
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10023..10127)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cgctagcgct accggtcgcc
     1021 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1081 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1141 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1201 accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
     1261 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1321 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     1381 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1441 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     1501 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1561 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1621 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1681 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1741 tccggagggg atcggtggag ctccaccggt ggcggccgct ctagagagaa tctttatttt
     1801 cagggcgccg ccaaattcaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
     1861 agcggaggtg gaggtactag ttctgtagac ccaatgacct acgaggccca gttctttggc
     1921 ttcacgccac aaacgtgcat gcttcggatc tacattgcat ttcaagacta cctatttgaa
     1981 gtgatgcagg ccgttgaaca ggttattctg aagaagctgg atggcatccc agactgtgac
     2041 attagcccag tgcagattcg caaatgcaca gagaagtttc tttgcttcat gaaaggacat
     2101 tttgataacc tttttagcaa aatggagcaa ctgtttttgc agctgatttt acgtattccc
     2161 tcaaacatct tgcttcctga agataaatgt aaggagacac cttatagtga ggaagatttt
     2221 cagcatctcc agaaagaaat tgaacagtta caggagaagt acaagactga attatgtact
     2281 aagcaggccc ttcttgcaga attagaagag caaaaaattg ttcaggccaa actcaaacag
     2341 acgttgactt tctttgatga gcttcataat gttggcagag atcatgggac tagtgatttt
     2401 agggagagtt tagtatccct ggttcagaac tccagaaaac tacagaacat tagagacaat
     2461 gtggaaaagg aatcgaaacg actgaaaata tctctcgaga tggacacccc ggaaaatgtc
     2521 cttcagatgc ttgaagccca catgcagagc tacaagggca atgaccctct tggtgaatgg
     2581 gaaagataca tacagtgggt agaagagaat tttcctgaga ataaagaata cttgataact
     2641 ttactagaac atttaatgaa ggaattttta gataagaaga aataccacaa tgacccaaga
     2701 ttcatcagtt attgtttaaa atttgctgag tacaacagtg acctccatca attttttgag
     2761 tttctgtaca accatgggat tggaaccctg tcatcccctc tgtacattgc ctgggcgggg
     2821 catctggaag cccaaggaga gctgcagcat gccagtgctg tccttcagag aggaattcaa
     2881 aaccaggctg aacccagaga gttcctgcaa caacaataca ggttatttca gacacgcctc
     2941 actgaaaccc atttgccagc tcaagctaga acctcagaac ctctgcataa tgttcaggtt
     3001 ttaaatcaaa tgataacatc aaaatcaaat ccaggaaata acatggcctg catttctaag
     3061 aatcagggtt cagagctttc tggagtgata tcttcagctt gtgataaaga gtcaaatatg
     3121 gaacgaagag tgattaccat ttctaaatca gaatattctg tgcactcatc tttggcatcc
     3181 aaagttgatg ttgagcaggt tgttatgtat tgcaaggaga agcttattcg tggggaatca
     3241 gaattttcct ttgaagaatt gagagcccag aaatacaatc aacggagaaa gcatgagcaa
     3301 tgggtaaatg aagacagaca ttatatgaaa aggaaagaag caaatgcttt tgaagaacag
     3361 ctattaaaac agaaaatgga tgaacttcat aagaagttgc atcaggtggt ggagacatcc
     3421 catgaggatc tgcccgcttc ccaggaaagg tccgaggtta atccagcacg tatggggcca
     3481 agtgtaggct cccagcagga actgagagcg ccatgtcttc cagtaaccta tcagcagaca
     3541 ccagtgaaca tggaaaagaa cccaagagag gcacctcctg ttgttcctcc tttggcaaat
     3601 gctatttctg cagctttggt gtccccagcc accagccaga gcattgctcc tcctgttcct
     3661 ttgaaagccc agacagtaac agactccatg tttgcagtgg ccagcaaaga tgctggatgt
     3721 gtgaataaga gtactcatga attcaagcca cagagtggag cagagatcaa agaagggtgt
     3781 gaaacacata aggttgccaa cacaagttct tttcacacaa ctccaaacac atcactggga
     3841 atggttcagg caacgccatc caaagtgcag ccatcaccca ccgtgcacac aaaagaagca
     3901 ttaggtttca tcatgaatat gtttcaggct cctacacttc ctgatatttc tgatgacaaa
     3961 gatgaatggc aatctctaga tcaaaatgaa gatgcatttg aagcccagtt tcaaaaaaat
     4021 gtaaggtcat ctggggcttg gggagtcaat aagatcatct cttctttgtc atctgctttt
     4081 catgtgtttg aagatggaaa caaagaaaat tatggattac cacagcctaa aaataaaccc
     4141 acaggagcca ggacctttgg agaacgctct gtcagcagac ttccttcaaa accaaaggag
     4201 gaagtgcctc atgctgaaga gtttttggat gactcaactg tatggggtat tcgctgcaac
     4261 aaaaccctgg cacccagtcc taagagccca ggagacttca catctgctgc acaacttgcg
     4321 tctacaccat tccacaagct tccagtggag tcagtgcaca ttttagaaga taaagaaaat
     4381 gtggtagcaa aacagtgtac ccaggcgact ttggattctt gtgaggaaaa catggtggtg
     4441 ccttcaaggg atggaaaatt cagtccaatt caagagaaaa gcccaaaaca ggccttgtcg
     4501 tctcacatgt attcagcatc cttacttcgt ctgagccagc ctgctgcagg tggggtactt
     4561 acctgtgagg cagagttggg cgttgaggct tgcagactca cagacactga cgctgccatt
     4621 gcagaagatc caccagatgc tattgctggg ctccaagcag aatggatgca gatgagttca
     4681 cttgggactg ttgatgctcc aaacttcatt gttgggaacc catgggatga taagctgatt
     4741 ttcaaacttt tatctgggct ttctaaacca gtgagttcct atccaaatac ttttgaatgg
     4801 caatgtaaac ttccagccat caagcccaag actgaatttc aattgggttc taagctggtc
     4861 tatgtccatc accttcttgg agaaggagcc tttgcccagg tgtacgaagc tacccaggga
     4921 gatctgaatg atgctaaaaa taaacagaaa tttgttttaa aggtccaaaa gcctgccaac
     4981 ccctgggaat tctacattgg gacccagttg atggaaagac taaagccatc tatgcagcac
     5041 atgtttatga agttctattc tgcccactta ttccagaatg gcagtgtatt agtaggagag
     5101 ctctacagct atggaacatt attaaatgcc attaacctct ataaaaatac ccctgaaaaa
     5161 gtgatgcctc aaggtcttgt catctctttt gctatgagaa tgctttacat gattgagcaa
     5221 gtgcatgact gtgaaatcat tcatggagac attaaaccag acaatttcat acttggaaac
     5281 ggatttttgg aacaggatga tgaagatgat ttatctgctg gcttggcact gattgacctg
     5341 ggtcagagta tagatatgaa actttttcca aaaggaacta tattcacagc aaagtgtgaa
     5401 acatctggtt ttcagtgtgt tgagatgctc agcaacaaac catggaacta ccagatcgat
     5461 tactttgggg ttgctgcaac agtatattgc atgctctttg gcacttacat gaaagtgaaa
     5521 aatgaaggag gagagtgtaa gcctgaaggt ctttttagaa ggcttcctca tttggatatg
     5581 tggaatgaat tttttcatgt tatgttgaat attccagatt gtcatcatct tccatctttg
     5641 gatttgttaa ggcaaaagct gaagaaagta tttcaacaac actatactaa caagattagg
     5701 gccctacgta ataggctaat tgtactgctc ttagaatgta agcgttcacg aaaataagga
     5761 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     5821 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     5881 gttgttaacc aaatggccaa gggaaccact gaagaaatga aatatgttct gggccaactt
     5941 gttggtctga attctcctaa ctccattttg aaagctgcta aaactttata tgaacactat
     6001 agtggtggtg aaagtcataa ttcttcatcc tccaagactt ttgaaaaaaa aaggggaaaa
     6061 aaatgagtcg agcatgcatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     6121 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6181 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     6241 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6301 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     6361 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6421 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6481 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6541 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6601 tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt
     6661 ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     6721 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     6781 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     6841 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     6901 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     6961 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     7021 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     7081 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     7141 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     7201 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     7261 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     7321 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     7381 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     7441 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     7501 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     7561 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     7621 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     7681 cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat
     7741 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     7801 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     7861 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7921 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     7981 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8041 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8101 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8161 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8221 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8281 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8341 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8401 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8461 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8521 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8581 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8641 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8701 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8761 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8821 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8881 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8941 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9001 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9061 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9121 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9181 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9241 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9301 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9361 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9421 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9481 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9541 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9601 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9661 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9721 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9781 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9841 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9901 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9961 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10021 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10081 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10141 aaagtgccac ctgacgtc
//
