LOCUS       sequence-218811-        2383 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Udawatte DJ, Lang DM, Currier JR, Medin CL, Rothman AL
  TITLE     Dengue virus downregulates TNFR1- and TLR3-stimulated NF-kappaB 
            activation by targeting RIPK1.
  JOURNAL   Front Cell Infect Microbiol. 2022 Oct 14;12:926036. doi: 
            10.3389/fcimb.2022.926036. eCollection 2022.
  PUBMED    36310878
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2022-10-14"; volume: "12:926036. doi"; 
            pages: " 10.3389/fcimb.2022.926036. eCollection 2022"
COMMENT     Sequence Label: pcDNA_DENV2-NS2B3_V5
FEATURES             Location/Qualifiers
     source          1..2383
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(2259..2283)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2309..2350
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tcgaaccatg gcaagctggc cattaaatga ggctatcatg gcagtcggga tggtgagcat
       61 tttggccagt tcactcctaa agaatgacat tcccatgaca ggaccattag tggctggagg
      121 gctcctcact gtgtgctacg tgctcactgg acgatcggcc gatttggaac tggagagagc
      181 cgccgatgtc aaatgggaag atcaggcaga gatatcagga agcagtccaa tcctgtcaat
      241 aacaatatca gaagatggta gcatgtcgat aaaaaacgaa gaggaagaac aaacactgac
      301 catactcatt agaacaggat tgctggtgat ctcaggactt tttcctgtat caataccaat
      361 cacggcagca gcatggtacc tgtgggaagt gaagaaacaa cgggctggag tattgtggga
      421 tgtcccttca cccccacccg tgggaaaggc tgaactggaa gatggagcct atagaatcaa
      481 gcaaaaaggg attcttggat attcccagat cggagccgga gtttacaaag aaggaacatt
      541 ccatacaatg tggcatgtca cacgcggcgc tgttctaatg cataaaggaa agaggattga
      601 accatcatgg gcggacgtta agaaagacct aatatcatat ggaggaggct ggaagctaga
      661 aggagaatgg aaggaaggag aagaagtcca ggtcttggca ttggagcctg gaaaaaatcc
      721 aagagccgtc caaacaaaac ctggtctttt caaaaccaac gccggaacca taggtgccgt
      781 atctctggac ttttctcctg gaacctcagg atctccaatc atcgacaaaa aaggaaaagt
      841 tgtgggtctt tatggtaatg gtgttgttac aaggagtggg gcatatgtga gtgctatagc
      901 ccagactgaa aaaagtattg aagacaatcc agagatcgaa gatgacattt ttcgaaagag
      961 aaaattgacc atcatggacc tccacccagg agcgggaaag acgaagagat accttccggc
     1021 catagtcaga gaggctataa aacggggcct gaggacatta atcctggccc ccactagagt
     1081 cgtggcagct gaaatggagg aagccctaag aggacttcca ataagatacc aaaccccagc
     1141 catcagagct gagcacaccg ggcgggagat tgtggaccta atgtgtcatg ccacattcac
     1201 tatgaggctg ctatcaccag ttagagtgcc aaattacaac ctgatcatca tggacgaagc
     1261 ccatttcaca gacccagcaa gtatagcggc tagaggatac atctcaactc gagtagagat
     1321 gggtgaggta gctgggattt tcatgacagc cactcctccg ggaagcagag acccattccc
     1381 tcagagcaat gcaccaatca tggatgaaga aagagaaatc cctgaacgtt cgtggagttc
     1441 tggacatgag tgggtcacgg attttaaagg gaagactgtt tggttcgttc caagtataaa
     1501 agcaggaaat gatatagcag cttgcctgag aaaaaatgga aagaaagtga tacaactcag
     1561 taggaagacc tttgattctg agtatgtcaa gactagaacc aatgattggg acttcgtggt
     1621 cacaactgac atttcagaaa tgggtgccaa cttcaaggct gagagggtta tagaccccag
     1681 acgctgcatg aaaccagtta tactaacaga tggtgaagag cgggtgatcc tggcaggacc
     1741 tatgccagtg acccactcta gtgcagcaca aagaagaggg agaataggaa gaaatccaaa
     1801 aaatgaaaat gaccagtaca tatacatggg ggaacctctg gaaaatgatg aagactgtgc
     1861 acactggaaa gaagctaaaa tgctcctaaa taacatcaac acacctgaag gaatcattcc
     1921 tagcatgttc gaaccagggc gtgaaaaggt ggatgccatt gatggtgaat accgcttgag
     1981 aggagaagca aggaaaacct ttgtggacct aatgagaaga ggagacctac cagtctggtt
     2041 ggcctacaga gtggcagctg aaggcatcaa ctacgcagac agaaggtggt gttttgatgg
     2101 aattaagaac aaccaaatct tggaagaaaa tgtggaggtg gaaatctgga caaaagaagg
     2161 ggaaaggaag aaattaaaac ccagatggtt ggatgccagg atctactctg acccactggc
     2221 gctaaaggaa ttcaaggaat ttgcagccgg aagaaaggac ccagctttct tgtacaaagt
     2281 ggttgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     2341 cgattctacg cgtaccggtt agtaatgagt ttaaacgggg gag
//
