LOCUS       sequence-218815-       12398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the TFDP1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Rady B, Chen Y, Vaca P, Wang Q, Wang Y, Salmon P, Oberholzer J
  TITLE     Overexpression of E2F3 promotes proliferation of functional human 
            beta cells without induction of apoptosis.
  JOURNAL   Cell Cycle. 2013 Aug 15;12(16):2691-702. doi: 10.4161/cc.25834. Epub
            2013 Jul 29.
  PUBMED    23907129
REFERENCE   2  (bases 1 to 12398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/cc.25834"; 
            journalName: "Cell Cycle"; date: "2013-08-15- 15"; volume: "12"; 
            issue: "16"; pages: "2691-702"
COMMENT     Sequence Label: pWPI-TFDP1
FEATURES             Location/Qualifiers
     source          1..12398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    48..165
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    197..221
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1461..1485)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1509..2076
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1527..2078
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1671..1688)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1898..1917
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2081..2083,2084..2086,2087..2797)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2081..2083,2084..2086,2087..2797)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2126..2147)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2387..2406)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2734..2755
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2876..2892)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2877..2893)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    2906..3494
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2959..2979)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3377..3388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3638..3671)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             3691..3871
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3896..3914)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3897..3914)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4204..4223)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4437..4456)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4556..4578
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4616..4634)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4702..4806
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4807..4875,4876..5667)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5025..5044)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5838..6426
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6327..6346
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6580..6597
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6667..6687)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6672..7001
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6852..6987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8135..8200
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8330..8350
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8775..8909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8812..8831)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8866..8885
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9077..9710
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9757..9882
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10375..10608
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10793..10837
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11220..12398
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          11451..12389
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     12346..12366
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggaatttcga catttaaatt taattaatct cgacggtatc ggttaacttt taaaagaaaa
       61 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      121 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact
      181 agcctcgagg tttatcacaa gtttgtacaa aaaagcaggc tccaccatgg caaaagatgc
      241 cggtctaatt gaagccaacg gagaactcaa ggtcttcata gaccagaacc ttagtcccgg
      301 gaaaggcgtg gtgtccctcg tggccgttca cccctccacc gtcaacccgc tcgggaagca
      361 gctcttgcca aaaacctttg gacagtccaa tgtcaacatt gcccagcaag tggtaattgg
      421 tacgcctcag agaccggcag cgtcaaacac cctggtggta ggaagcccac acacccccag
      481 cactcacttt gcctctcaga accagccttc cgactcctca ccttggtctg ccgggaagcg
      541 caacaggaaa ggagagaaga atggcaaggg cctacggcat ttctccatga aggtctgcga
      601 gaaggtgcag aggaaaggga ccacttccta caacgaagtg gcagacgagc tggttgcgga
      661 gttcagtgct gccgacaacc acatcttacc aaacgagtca gcttatgacc agaaaaacat
      721 aagacggcgc gtctacgatg ccttaaacgt gctaatggcc atgaacatca tctccaagga
      781 gaagaaggag atcaagtgga ttggtctgcc caccaactcg gctcaggaat gtcagaactt
      841 agaggtggaa agacagagga gacttgaaag aataaaacag aaacagtctc aacttcaaga
      901 acttattcta cagcaaattg ccttcaagaa cctggtgcag agaaaccggc atgcggagca
      961 gcaggccagc cggccaccgc cacccaactc agtcatccac ctgcccttca tcatcgtcaa
     1021 caccagcaag aagacggtca tcgactgcag catctccaat gacaaatttg agtatctgtt
     1081 taattttgac aacacatttg aaatccacga tgacatagaa gtgctgaagc ggatgggcat
     1141 ggcttgcggg ctggagtcgg ggagctgctc tgccgaagac cttaaaatgg ccagaagtct
     1201 ggtccccaag gctctggagc catacgtgac agaaatggct cagggaactg ttggaggcgt
     1261 gttcatcacg acggcaggtt ccacgtctaa cggcacaagg ttctctgcca gtgacctgac
     1321 caacggtgca gatgggatgc tggccacaag ctccaatggg tctcagtaca gcggctccag
     1381 ggtggagact ccggtgtcct acgtcgggga ggacgacgag gaggacgatg acttcaacga
     1441 gaatgacgag gacgactagg acccagcttt cttgtacaaa gtggtgataa actacgggct
     1501 gcaggaattc cgcccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     1561 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     1621 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     1681 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     1741 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     1801 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     1861 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     1921 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     1981 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac
     2041 ggggacgtgg ttttcctttg aaaaacacga tgataatacc atggtgagca agggcgagga
     2101 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2161 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2221 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2281 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2341 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2401 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2461 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2521 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2581 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2641 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2701 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2761 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgactagc
     2821 tagtagctag ctagctagtc gagctcaagc ttcgaattcg atatcaagct tatcgcgata
     2881 ccgtcgacct cgagggaatt ccgataatca acctctggat tacaaaattt gtgaaagatt
     2941 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3001 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3061 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3121 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3181 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3241 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3301 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3361 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3421 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3481 ggccgcctcc ccgcatcggg aattcgagct cggtaccttt aagaccaatg acttacaagg
     3541 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3601 cccaacgaag acaagatggg atcaattcac catgggaata acttcgtata gcatacatta
     3661 tacgaagtta tgctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     3721 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     3781 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     3841 cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat
     3901 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
     3961 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat
     4021 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg
     4081 gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc
     4141 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     4201 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     4261 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     4321 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     4381 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca
     4441 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4501 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4561 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4621 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4681 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4741 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4801 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4861 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4921 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4981 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5041 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5101 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5161 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5221 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5281 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5341 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5401 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5461 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5521 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5581 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5641 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5701 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5761 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5821 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5881 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5941 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6001 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6061 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6121 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6181 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6241 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6301 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6361 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6421 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6481 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6541 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6601 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6661 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6721 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6781 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6841 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6901 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6961 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
     7021 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
     7081 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
     7141 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
     7201 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
     7261 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
     7321 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     7381 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
     7441 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
     7501 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     7561 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
     7621 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     7681 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
     7741 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
     7801 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     7861 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     7921 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     7981 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
     8041 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     8101 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     8161 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     8221 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     8281 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     8341 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     8401 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     8461 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     8521 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     8581 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     8641 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     8701 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     8761 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8821 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8881 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     8941 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     9001 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     9061 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     9121 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     9181 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     9241 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     9301 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     9361 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     9421 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     9481 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     9541 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9601 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9661 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     9721 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     9781 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     9841 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     9901 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     9961 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    10021 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    10081 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    10141 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    10201 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
    10261 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    10321 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    10381 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    10441 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    10501 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    10561 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    10621 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    10681 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    10741 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    10801 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    10861 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    10921 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    10981 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
    11041 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc
    11101 acgagactag cctcgaccat cgatgtcgac gataagcttt gcaaagatgg ataaagtttt
    11161 aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg
    11221 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
    11281 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
    11341 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
    11401 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
    11461 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
    11521 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
    11581 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
    11641 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
    11701 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
    11761 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
    11821 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
    11881 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
    11941 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
    12001 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
    12061 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
    12121 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
    12181 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
    12241 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
    12301 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
    12361 tggttcaaag tttttttctt ccatttcagg tgtcgtga
//
