LOCUS       sequence-218818-        5504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ATF1 from Saccharomyces cerevisiae under osmY stationary phase 
            promoter; kanamycin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, 
            Bryk J
  TITLE     UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene
            expression control and logic in E. coli
  JOURNAL   bioRxiv 2021.06.20.449138
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.06.20.449138"
COMMENT     Sequence Label: p006-Banana-Late
FEATURES             Location/Qualifiers
     source          1..5504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1083
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(20..39)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      1169..1757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1658..1677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1911..1928
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2026..2043
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2027..2043
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4216..4287
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4303..4330
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(4435..4454)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        4521..4612
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4613..5422
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 atgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
       61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      481 ttctcccatg aggacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agatagctca
      841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgcca
      961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1081 tgactcatga ccaaaatccc ttaacgtgag ttacgcgcgc gtcgttccac tgagcgtcag
     1141 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1201 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1261 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1321 tagtgtagcc gtagttagcc caccacttca agaactctgt agcaccgcct acatacctcg
     1381 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1441 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1501 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1561 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1621 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1681 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1741 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1801 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1861 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     1921 tgagcgagga agcggaaggc gagagtaggg aactgccagg catcaaacta agcagaaggc
     1981 ccctgacgga tggccttttt gcgtttctac aaactctttc tgtgttgtaa aacgacggcc
     2041 agtcttaagc tcgggccccc tgggcggttc tgataacgag taatcgttaa tccgcaaata
     2101 acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt
     2161 cagaatattt aagggcgcct gtcactttgc ttgatatatg agaattattt aaccttataa
     2221 atgagaaaaa agcaacgcac tttaaataag atacgttgct ttttcgattg atgaacacct
     2281 ataattaaac tattcatcta ttatttatga ttttttgtat atacaatatt tctagtttgt
     2341 taaagagaat taagaaaata aatctcgaaa ataataaagg gaaaatcagt tttctggcac
     2401 aggaacgtta tccggacgtt cagttccacc agacccgcga gcattaattc ttgcctccag
     2461 ggcgcggtag tggcgccctg tcaatttccc ttccttatta gccgcttacg gaatgttctt
     2521 aaaacattca cttttgctta tgttttcgct gatatcccga gcggtttcaa aattgtgatc
     2581 tatatttaac aatactagag aaagagggga aatactagat gaatgaaatc gatgagaaaa
     2641 atcaggcccc cgtgcaacaa gaatgcctga aagagatgat tcagaatggg catgctcggc
     2701 gtatgggatc tgttgaagat ctgtatgttg ctctcaacag acaaaactta tatcgaaact
     2761 tctgcacata tggagaattg agtgattact gtactaggga tcagctcaca ttagctttga
     2821 gggaaatctg cctgaaaaat ccaactcttt tacatattgt tctaccaaca agatggccaa
     2881 atcatgaaaa ttattatcgc agttccgaat actattcacg gccacatcca gtgcatgatt
     2941 atatttcagt attacaagaa ttgaaactga gtggtgtggt tctcaatgaa caacctgagt
     3001 acagtgcagt aatgaagcaa atattagaag agttcaaaaa tagtaagggt tcctatactg
     3061 caaaaatttt taaacttact accactttga ctattcctta ctttggacca acaggaccga
     3121 gttggcggct aatttgtctt ccagaagagc acacagaaaa gtggaaaaaa tttatctttg
     3181 tatctaatca ttgcatgtct gatggtcggt cttcgatcca cttttttcat gatttaagag
     3241 acgaattaaa taatattaaa actccaccaa aaaaattaga ttacattttc aagtacgagg
     3301 aggattacca attattgagg aaacttccag aaccgatcga aaaggtgata gactttagac
     3361 caccgtactt gtttattccg aagtcacttc tttcgggttt catctacaat catttgagat
     3421 tttcttcaaa aggtgtctgt atgagaatgg atgatgtgga aaaaaccgat gatgttgtca
     3481 ccgagatcat caatatttca ccaacagaat ttcaagcgat taaagcaaat attaaatcaa
     3541 atatccaagg taagtgtact atcactccgt ttttacatgt ttgttggttt gtatctcttc
     3601 ataaatgggg taaatttttc aaaccattga acttcgaatg gcttacggat atttttatcc
     3661 ccgcagattg ccgctcacaa ctaccagatg atgatgaaat gagacagatg tacagatatg
     3721 gcgctaacgt tggatttatt gacttcaccc cctggataag cgaatctgac atgaatgata
     3781 acaaagaaaa tttttggcca cttattgagc actaccatga agtaatttcg gaagctttaa
     3841 gaaataaaaa gcatctccat ggcttagggt tcaatataca aggcttcgtt caaaaatatg
     3901 tgaacattga caaggtaatg tgcgatcgtg ccatcgggaa aagacgcgga ggtacattgt
     3961 taagcaatgt aggtctgttt aatcagttag aggagcccga tgccaaatat tctatatgcg
     4021 atttggcatt tggccaattt caaggatcct ggcaccaagc attttccttg ggtgtttgtt
     4081 cgactaatgt aaaggggatg aatattgttg ttgcttcaac aaagaatgtt gttggtagtc
     4141 aagaatctct cgaagagctt tgctccattt acaaagctct ccttttaggc ccttaataat
     4201 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     4261 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     4321 cctttctgcg tttatacatg gggagtcccc acactaccat cggcgctacg gcgtttcact
     4381 tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg caaacaaggg
     4441 gtgttatgag ccatattcag gtataaatgg gctcgcgata atgttcagaa ttggttaatt
     4501 ggttgtaaca ctgaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4561 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga atatgagcca
     4621 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     4681 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     4741 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     4801 tacagatgag atggtcagac taaactggct gacggaattt atgccacttc cgaccatcaa
     4861 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
     4921 agcgttccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     4981 agtgttcctg cgccggttgc actcgattcc tgtttgtaat tgtcctttta acagcgatcg
     5041 cgtatttcgc ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     5101 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     5161 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     5221 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     5281 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     5341 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc aatttcattt
     5401 gatgctcgat gagtttttct aagcggcgcg ccatcgaatg gcgcaaaacc tttcgcggta
     5461 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaat
//
