LOCUS       sequence-218826-       10260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human LRP5 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Lai MB, Zhang C, Shi J, Johnson V, Khandan L, McVey J, Klymkowsky 
            MW, Chen Z, Junge HJ
  TITLE     TSPAN12 Is a Norrin Co-receptor that Amplifies Frizzled4 Ligand 
            Selectivity and Signaling.
  JOURNAL   Cell Rep. 2017 Jun 27;19(13):2809-2822. doi: 
            10.1016/j.celrep.2017.06.004.
  PUBMED    28658627
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-06-27- 27"; volume: "19"; issue: "13"; pages: "2809-2822"
COMMENT     Sequence Label: pcDNA 3.3 LRP5
FEATURES             Location/Qualifiers
     source          1..10260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5646..5665)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5690..5738
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5940..6368
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6027..6046)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6237..6258
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6377..6397)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6382..6711
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6562..6697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6624..6643
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6778..7572
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6832..6851)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7442..7461
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7748..7869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7785..7804)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7839..7858
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7918..7934)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7918..7934)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7931..7953)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7942..7958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7966..7972,7973..7990,7991..7996))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8011..8032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8149..8166)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8320..8908)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8400..8419)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9079..9870,9871..9939))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9702..9721
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9940..10044)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10119..10138)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagg atcgaaccct tccaccatgg aggcagcgcc gcccgggccg ccgtggccgc
      781 tgctgctgct gctgctgctg ctgctggcgc tgtgcggctg cccggccccc gccgcggcct
      841 cgccgctcct gctatttgcc aaccgccggg acgtacggct ggtggacgcc ggcggagtca
      901 agctggagtc caccatcgtg gtcagcggcc tggaggatgc ggccgcagtg gacttccagt
      961 tttccaaggg agccgtgtac tggacagacg tgagcgagga ggccatcaag cagacctacc
     1021 tgaaccagac gggggccgcc gtgcagaacg tggtcatctc cggcctggtc tctcccgacg
     1081 gcctcgcctg cgactgggtg ggcaagaagc tgtactggac ggactcagag accaaccgca
     1141 tcgaggtggc caacctcaat ggcacatccc ggaaggtgct cttctggcag gaccttgacc
     1201 agccgagggc catcgccttg gaccccgctc acgggtacat gtactggaca gactggggtg
     1261 agacgccccg gattgagcgg gcagggatgg atggcagcac ccggaagatc attgtggact
     1321 cggacattta ctggcccaat ggactgacca tcgacctgga ggagcagaag ctctactggg
     1381 ctgacgccaa gctcagcttc atccaccgtg ccaacctgga cggctcgttc cggcagaagg
     1441 tggtggaggg cagcctgacg caccccttcg ccctgacgct ctccggggac actctgtact
     1501 ggacagactg gcagacccgc tccatccatg cctgcaacaa gcgcactggg gggaagagga
     1561 aggagatcct gagtgccctc tactcaccca tggacatcca ggtgctgagc caggagcggc
     1621 agcctttctt ccacactcgc tgtgaggagg acaatggcgg ctgctcccac ctgtgcctgc
     1681 tgtccccaag cgagcctttc tacacatgcg cctgccccac gggtgtgcag ctgcaggaca
     1741 acggcaggac gtgtaaggca ggagccgagg aggtgctgct gctggcccgg cggacggacc
     1801 tacggaggat ctcgctggac acgccggact tcaccgacat cgtgctgcag gtggacgaca
     1861 tccggcacgc cattgccatc gactacgacc cgctagaggg ctatgtctac tggacagatg
     1921 acgaggtgcg ggccatccgc agggcgtacc tggacgggtc tggggcgcag acgctggtca
     1981 acaccgagat caacgacccc gatggcatcg cggtcgactg ggtggcccga aacctctact
     2041 ggaccgacac gggcacggac cgcatcgagg tgacgcgcct caacggcacc tcccgcaaga
     2101 tcctggtgtc ggaggacctg gacgagcccc gagccatcgc actgcacccc gtgatgggcc
     2161 tcatgtactg gacagactgg ggagagaacc ctaaaatcga gtgtgccaac ttggatgggc
     2221 aggagcggcg tgtgctggtc aatgcctccc tcgggtggcc caacggcctg gccctggacc
     2281 tgcaggaggg gaagctctac tggggagacg ccaagacaga caagatcgag gtgatcaatg
     2341 ttgatgggac gaagaggcgg accctcctgg aggacaagct cccgcacatt ttcgggttca
     2401 cgctgctggg ggacttcatc tactggactg actggcagcg ccgcagcatc gagcgggtgc
     2461 acaaggtcaa ggccagccgg gacgtcatca ttgaccagct gcccgacctg atggggctca
     2521 aagctgtgaa tgtggccaag gtcgtcggaa ccaacccgtg tgcggacagg aacggggggt
     2581 gcagccacct gtgcttcttc acaccccacg caacccggtg tggctgcccc atcggcctgg
     2641 agctgctgag tgacatgaag acctgcatcg tgcctgaggc cttcttggtc ttcaccagca
     2701 gagccgccat ccacaggatc tccctcgaga ccaataacaa cgacgtggcc atcccgctca
     2761 cgggcgtcaa ggaggcctca gccctggact ttgatgtgtc caacaaccac atctactgga
     2821 cagacgtcag cctgaagacc atcagccgcg ccttcatgaa cgggagctcg gtggagcacg
     2881 tggtggagtt tggccttgac taccccgagg gcatggccgt tgactggatg ggcaagaacc
     2941 tctactgggc cgacactggg accaacagaa tcgaagtggc gcggctggac gggcagttcc
     3001 ggcaagtcct cgtgtggagg gacttggaca acccgaggtc gctggccctg gatcccacca
     3061 agggctacat ctactggacc gagtggggcg gcaagccgag gatcgtgcgg gccttcatgg
     3121 acgggaccaa ctgcatgacg ctggtggaca aggtgggccg ggccaacgac ctcaccattg
     3181 actacgctga ccagcgcctc tactggaccg acctggacac caacatgatc gagtcgtcca
     3241 acatgctggg tcaggagcgg gtcgtgattg ccgacgatct cccgcacccg ttcggtctga
     3301 cgcagtacag cgattatatc tactggacag actggaatct gcacagcatt gagcgggccg
     3361 acaagactag cggccggaac cgcaccctca tccagggcca cctggacttc gtgatggaca
     3421 tcctggtgtt ccactcctcc cgccaggatg gcctcaatga ctgtatgcac aacaacgggc
     3481 agtgtgggca gctgtgcctt gccatccccg gcggccaccg ctgcggctgc gcctcacact
     3541 acaccctgga ccccagcagc cgcaactgca gcccgcccac caccttcttg ctgttcagcc
     3601 agaaatctgc catcagtcgg atgatcccgg acgaccagca cagcccggat ctcatcctgc
     3661 ccctgcatgg actgaggaac gtcaaagcca tcgactatga cccactggac aagttcatct
     3721 actgggtgga tgggcgccag aacatcaagc gagccaagga cgacgggacc cagccctttg
     3781 ttttgacctc tctgagccaa ggccaaaacc cagacaggca gccccacgac ctcagcatcg
     3841 acatctacag ccggacactg ttctggacgt gcgaggccac caataccatc aacgtccaca
     3901 ggctgagcgg ggaagccatg ggggtggtgc tgcgtgggga ccgcgacaag cccagggcca
     3961 tcgtcgtcaa cgcggagcga gggtacctgt acttcaccaa catgcaggac cgggcagcca
     4021 agatcgaacg cgcagccctg gacggcaccg agcgcgaggt cctcttcacc accggcctca
     4081 tccgccctgt ggccctggtg gtagacaaca cactgggcaa gctgttctgg gtggacgcgg
     4141 acctgaagcg cattgagagc tgtgacctgt caggggccaa ccgcctgacc ctggaggacg
     4201 ccaacatcgt gcagcctctg ggcctgacca tccttggcaa gcatctctac tggatcgacc
     4261 gccagcagca gatgatcgag cgtgtggaga agaccaccgg ggacaagcgg actcgcatcc
     4321 agggccgtgt cgcccacctc actggcatcc atgcagtgga ggaagtcagc ctggaggagt
     4381 tctcagccca cccatgtgcc cgtgacaatg gtggctgctc ccacatctgt attgccaagg
     4441 gtgatgggac accacggtgc tcatgcccag tccacctcgt gctcctgcag aacctgctga
     4501 cctgtggaga gccgcccacc tgctccccgg accagtttgc atgtgccaca ggggagatcg
     4561 actgtatccc cggggcctgg cgctgtgacg gctttcccga gtgcgatgac cagagcgacg
     4621 aggagggctg ccccgtgtgc tccgccgccc agttcccctg cgcgcggggt cagtgtgtgg
     4681 acctgcgcct gcgctgcgac ggcgaggcag actgtcagga ccgctcagac gaggcggact
     4741 gtgacgccat ctgcctgccc aaccagttcc ggtgtgcgag cggccagtgt gtcctcatca
     4801 aacagcagtg cgactccttc cccgactgta tcgacggctc cgacgagctc atgtgtgaaa
     4861 tcaccaagcc gccctcagac gacagcccgg cccacagcag tgccatcggg cccgtcattg
     4921 gcatcatcct ctctctcttc gtcatgggtg gtgtctattt tgtgtgccag cgcgtggtgt
     4981 gccagcgcta tgcgggggcc aacgggccct tcccgcacga gtatgtcagc gggaccccgc
     5041 acgtgcccct caatttcata gccccgggcg gttcccagca tggccccttc acaggcatcg
     5101 catgcggaaa gtccatgatg agctccgtga gcctgatggg gggccggggc ggggtgcccc
     5161 tctacgaccg gaaccacgtc acaggggcct cgtccagcag ctcgtccagc acgaaggcca
     5221 cgctgtaccc gccgatcctg aacccgccgc cctccccggc cacggacccc tccctgtaca
     5281 acatggacat gttctactct tcaaacattc cggccactgc gagaccgtac aggccctaca
     5341 tcattcgagg aatggcgccc ccgacgacgc cctgcagcac cgacgtgtgt gacagcgact
     5401 acagcgccag ccgctggaag gccagcaagt actacctgga tttgaactcg gactcagacc
     5461 cctatccacc cccacccacg ccccacagcc agtacctgtc ggcggaggac agctgcccgc
     5521 cctcgcccgc caccgagagg agctacttcc atctcttccc gccccctccg tccccctgca
     5581 cggactcatc ctgaaagggt tcgatcccta ccggttagta atgagtttaa acgggggagg
     5641 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     5701 agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     5761 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     5821 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     5881 cggggcggca ggccctgcca tagcagatct gcgcagctgg ggctctaggg ggtatcccca
     5941 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6001 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6061 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag
     6121 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6181 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6241 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6301 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6361 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     6421 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     6481 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6541 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6601 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     6661 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     6721 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     6781 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     6841 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     6901 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     6961 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     7021 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     7081 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     7141 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     7201 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     7261 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     7321 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     7381 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     7441 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     7501 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     7561 gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct
     7621 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7681 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7741 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7801 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7861 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7921 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7981 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8041 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8101 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8161 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8221 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8281 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8341 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8401 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8461 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     8521 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8581 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8641 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8701 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8761 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8821 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8881 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8941 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9001 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9061 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9121 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9181 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9241 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9301 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9361 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9421 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9481 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9541 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9601 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9661 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9721 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9781 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9841 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9901 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9961 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10021 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    10081 atcgggagat ctcccgatcc cctatggtcg actctcagta caatctgctc tgatgccgca
    10141 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    10201 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
//
