LOCUS       sequence-21883-e         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: pET41a-MED22
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..73
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             89..133
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             164..178
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 aatcggatgg ttcactagtg gttctggtca tcaccatcac catcactccg cgggtctggt
       61 gccacgcggt agtactgcaa ttggtatgaa agaaaccgct gctgctaaat tcgaacgcca
      121 gcacatggac agcccagatc tgggtaccgg tggtggctcc ggtgatgacg acgacaagag
      181 tcccatggga tatcggggat ccaccatggc ccagcagaga gccctgcccc agagcaagga
      241 gacgctgctg cagtcctaca acaagcggct gaaggacgac attaagtcca tcatggacaa
      301 cttcaccgag atcatcaaga ccgccaagat tgaggacgag acgcaggtgt cacgggccac
      361 tcagggtgaa caggacaatt acgagatgca tgtgcgagcc gccaacatcg tccgagccgg
      421 cgagtccctg atgaagctgg tgtccgacct caagcagttc ctgatcctca atgacttccc
      481 ctccgtgaac gaggccattg accagcgcaa ccagcagctg cgcacactgc aggaggagtg
      541 cgaccggaag ctcatcacgc tgcgagacga gatctccatt gacctctacg agctggagga
//
