LOCUS       sequence-21884-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: pET41a-MED18
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..72
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             88..132
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             163..177
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 tcggatggtt caactagtgg ttctggtcat caccatcacc atcactccgc gggtctggtg
       61 ccacgcggta gtactgcaat tggtatgaaa gaaaccgctg ctgctaaatt cgaacgccag
      121 cacatggaca gcccagatct gggtaccggt ggtggctccg gtgatgacga cgacaagagt
      181 cccatgggat atcggggatc atggaggcac ctccagtcac catgatgcct gtcactgggg
      241 gcaccattaa catgatggag tacctgttgc agggaagtgt tttagatcac agtttggaaa
      301 gcctcatcca ccgccttcgt ggtttgtgtg acaacatgga acctgagact ttccttgacc
      361 atgagatggt attcctcctt aagggccagc aagccagccc atttgttctc agggcccgac
      421 gctctatgga cagggcaggg gcaccctggc atctgcgcta cctgggacag ccagaaatgg
      481 gagacaagaa ccgccatgcc ctggtgcgaa actgcgtgga cattgccaca tctgagaacc
      541 tcaccgactt cttgatggaa atgggcttcc gcatggacca tgagtttgtt gctaagggac
      601 atttgttccg taagggcatc atgaagatta tggtgtacaa gattttccgc atcctggtgc
      661 cagggaacac agacagcact ga
//
