LOCUS       sequence-218848-       12279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the hTert protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12279)
  AUTHORS   Salmon P
  TITLE     Generation of human cell lines using lentiviral-mediated genetic 
            engineering.
  JOURNAL   Methods Mol Biol. 2013;945:417-48. doi: 
            10.1007/978-1-62703-125-7_25.
  PUBMED    23097121
REFERENCE   2  (bases 1 to 12279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2013"; volume: "945:417-48. doi"; pages: " 
            10.1007/978-1-62703-125-7_25"
COMMENT     Sequence Label: pCWX-UBI-hTert-PGK-BSD
FEATURES             Location/Qualifiers
     source          1..12279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     262..281
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    432..456
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             551..3949
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     protein_bind    complement(3961..3985)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4002..4512
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4431..4449
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4527..4536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4533..4931
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4950..5538
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5003..5023)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5421..5432)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5541..5557)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5542..5558)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(5686..5719)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5739..5919
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5957..5976)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6022..6077
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6076..6095)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6438..6454)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6438..6454)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6451..6473)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6462..6478
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6486..6492,6493..6510,6511..6516))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6531..6552
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6611..6806
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6657..6792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6812..6946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6849..6868)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6903..6922
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7014..7031)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7185..7772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7265..7284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7943..8734,8735..8803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8566..8585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8804..8908)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8939..9318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             9320..9577
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9624..9749
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10241..10358
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10718..10951
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11136..11180
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    11455..11475
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        join(11493..12279,1..425)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt
       61 cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg
      121 cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg
      181 cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat
      241 gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga
      301 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
      361 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
      421 tagacaggat cccaactttg tacaaaaaag caggctacta gtgaggaacc aattcagtcg
      481 actggatccg gtaccgaatt aattcgtcct gctgcgcacg tgggaagccc tggccccggc
      541 cacccccgcg atgccgcgcg ctccccgctg ccgagccgtg cgctccctgc tgcgcagcca
      601 ctaccgcgag gtgctgccgc tggccacgtt cgtgcggcgc ctggggcccc agggctggcg
      661 gctggtgcag cgcggggacc cggcggcttt ccgcgcgctg gtggcccagt gcctggtgtg
      721 cgtgccctgg gacgcacggc cgccccccgc cgccccctcc ttccgccagg tgtcctgcct
      781 gaaggagctg gtggcccgag tgctgcagag gctgtgcgag cgcggcgcga agaacgtgct
      841 ggccttcggc ttcgcgctgc tggacggggc ccgcgggggc ccccccgagg ccttcaccac
      901 cagcgtgcgc agctacctgc ccaacacggt gaccgacgca ctgcggggga gcggggcgtg
      961 ggggctgctg ctgcgccgcg tgggcgacga cgtgctggtt cacctgctgg cacgctgcgc
     1021 gctctttgtg ctggtggctc ccagctgcgc ctaccaggtg tgcgggccgc cgctgtacca
     1081 gctcggcgct gccactcagg cccggccccc gccacacgct agtggacccc gaaggcgtct
     1141 gggatgcgaa cgggcctgga accatagcgt cagggaggcc ggggtccccc tgggcctgcc
     1201 agccccgggt gcgaggaggc gcgggggcag tgccagccga agtctgccgt tgcccaagag
     1261 gcccaggcgt ggcgctgccc ctgagccgga gcggacgccc gttgggcagg ggtcctgggc
     1321 ccacccgggc aggacgcgtg gaccgagtga ccgtggtttc tgtgtggtgt cacctgccag
     1381 acccgccgaa gaagccacct ctttggaggg tgcgctctct ggcacgcgcc actcccaccc
     1441 atccgtgggc cgccagcacc acgcgggccc cccatccaca tcgcggccac cacgtccctg
     1501 ggacacgcct tgtcccccgg tgtacgccga gaccaagcac ttcctctact cctcaggcga
     1561 caaggagcag ctgcggccct ccttcctact cagctctctg aggcccagcc tgactggcgc
     1621 tcggaggctc gtggagacca tctttctggg ttccaggccc tggatgccag ggactccccg
     1681 caggttgccc cgcctgcccc agcgctactg gcaaatgcgg cccctgtttc tggagctgct
     1741 tgggaaccac gcgcagtgcc cctacggggt gctcctcaag acgcactgcc cgctgcgagc
     1801 tgcggtcacc ccagcagccg gtgtctgtgc ccgggagaag ccccagggct ctgtggcggc
     1861 ccccgaggag gaggacacag acccccgtcg cctggtgcag ctgctccgcc agcacagcag
     1921 cccctggcag gtgtacggct tcgtgcgggc ctgcctgcgc cggctggtgc ccccaggcct
     1981 ctggggctcc aggcacaacg aacgccgctt cctcaggaac accaagaagt tcatctccct
     2041 ggggaagcat gccaagctct cgctgcagga gctgacgtgg aagatgagcg tgcggggctg
     2101 cgcttggctg cgcaggagcc caggggttgg ctgtgttccg gccgcagagc accgtctgcg
     2161 tgaggagatc ctggccaagt tcctgcactg gctgatgagt gtgtacgtcg tcgagctgct
     2221 caggtctttc ttttatgtca cggagaccac gtttcaaaag aacaggctct ttttctaccg
     2281 gaagagtgtc tggagcaagt tgcaaagcat tggaatcaga cagcacttga agagggtgca
     2341 gctgcgggag ctgtcggaag cagaggtcag gcagcatcgg gaagccaggc ccgccctgct
     2401 gacgtccaga ctccgcttca tccccaagcc tgacgggctg cggccgattg tgaacatgga
     2461 ctacgtcgtg ggagccagaa cgttccgcag agaaaagagg gccgagcgtc tcacctcgag
     2521 ggtgaaggca ctgttcagcg tgctcaacta cgagcgggcg cggcgccccg gcctcctggg
     2581 cgcctctgtg ctgggcctgg acgatatcca cagggcctgg cgcaccttcg tgctgcgtgt
     2641 gcgggcccag gacccgccgc ctgagctgta ctttgtcaag gtggatgtga cgggcgcgta
     2701 cgacaccatc ccccaggaca ggctcacgga ggtcatcgcc agcatcatca aaccccagaa
     2761 cacgtactgc gtgcgtcggt atgccgtggt ccagaaggcc gcccatgggc acgtccgcaa
     2821 ggccttcaag agccacgtct ctaccttgac agacctccag ccgtacatgc gacagttcgt
     2881 ggctcacctg caggagacca gcccgctgag ggatgccgtc gtcatcgagc agagctcctc
     2941 cctgaatgag gccagcagtg gcctcttcga cgtcttccta cgcttcatgt gccaccacgc
     3001 cgtgcgcatc aggggcaagt cctacgtcca gtgccagggg atcccgcagg gctccatcct
     3061 ctccacgctg ctctgcagcc tgtgctacgg cgacatggag aacaagctgt ttgcggggat
     3121 tcggcgggac gggctgctcc tgcgtttggt ggatgatttc ttgttggtga cacctcacct
     3181 cacccacgcg aaaaccttcc tcaggaccct ggtccgaggt gtccctgagt atggctgcgt
     3241 ggtgaacttg cggaagacag tggtgaactt ccctgtagaa gacgaggccc tgggtggcac
     3301 ggcttttgtt cagatgccgg cccacggcct attcccctgg tgcggcctgc tgctggatac
     3361 ccggaccctg gaggtgcaga gcgactactc cagctatgcc cggacctcca tcagagccag
     3421 tctcaccttc aaccgcggct tcaaggctgg gaggaacatg cgtcgcaaac tctttggggt
     3481 cttgcggctg aagtgtcaca gcctgtttct ggatttgcag gtgaacagcc tccagacggt
     3541 gtgcaccaac atctacaaga tcctcctgct gcaggcgtac aggtttcacg catgtgtgct
     3601 gcagctccca tttcatcagc aagtttggaa gaaccccaca tttttcctgc gcgtcatctc
     3661 tgacacggcc tccctctgct actccatcct gaaagccaag aacgcaggga tgtcgctggg
     3721 ggccaagggc gccgccggcc ctctgccctc cgaggccgtg cagtggctgt gccaccaagc
     3781 attcctgctc aagctgactc gacaccgtgt cacctacgtg ccactcctgg ggtcactcag
     3841 gacagcccag acgcagctga gtcggaagct cccggggacg acgctgactg ccctggaggc
     3901 cgcagccaac ccggcactgc cctcagactt caagaccatc ctggactgag tcgaatctag
     3961 acccagcttt cttgtacaaa gtggtgataa tcgaattcca cggggttggg gttgcgcctt
     4021 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
     4081 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
     4141 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
     4201 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact
     4261 agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct
     4321 gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg
     4381 cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca
     4441 ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac
     4501 ctctctcccc agggggatcc accggtgcca ccatggccaa gcctttgtct caagaagaat
     4561 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     4621 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     4681 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     4741 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     4801 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     4861 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     4921 gggagggcta agatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     4981 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5041 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5101 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5161 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5221 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5281 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5341 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5401 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5461 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5521 tttgggccgc ctccccgcat cgataccgtc gacctcgagg ttcgctttaa gaccaatgac
     5581 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5641 aattcactcc caacgaagac aagatgggat caattcacca tgggaataac ttcgtatagc
     5701 atacattata cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     5761 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5821 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5881 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagttg aatcgccagt
     5941 gtgtcgagct agcagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     6001 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     6061 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     6121 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     6181 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     6241 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     6301 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     6361 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     6421 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6481 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     6541 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6601 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     6661 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6721 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     6781 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta
     6841 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6901 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga
     6961 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7021 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7081 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7141 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7201 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7261 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7321 ctgccgctta cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7381 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7441 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7501 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7561 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7621 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7681 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7741 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7801 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7861 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7921 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7981 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8041 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8101 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8161 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8221 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8281 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8341 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8401 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8461 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8521 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8581 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8641 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8701 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8761 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8821 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8881 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
     8941 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     9001 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     9061 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     9121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     9181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     9241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     9301 gtcatcgcta ttaccatggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     9361 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     9421 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9481 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9541 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     9601 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     9661 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     9721 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     9781 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     9841 gggcaagcag ggagctagaa cgattcgcag ttaatactgg cctgttagaa acatcagaag
     9901 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     9961 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
    10021 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag aaaaaagcac
    10081 agcaagcagc aggatcttca gacctggaaa ttccctacaa tccccaaagt caaggagtag
    10141 tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac
    10201 atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    10261 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    10321 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    10381 gaaatccact ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa
    10441 tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc attagggatt
    10501 atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag gattagaaca
    10561 tggaaaagtt tagtaaaaca ccataaggag gagatatgag ggacaattgg agaagtgaat
    10621 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
    10681 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
    10741 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
    10801 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
    10861 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
    10921 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
    10981 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
    11041 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
    11101 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
    11161 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
    11221 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
    11281 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
    11341 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
    11401 acagatccat tcgattagtg aacggatctc gacggtatct agtgattatc ggatcaactt
    11461 tgtatagaaa agttgttaat taagggtgca gcggcctccg cgccgggttt tggcgcctcc
    11521 cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg
    11581 tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt
    11641 agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact
    11701 ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc
    11761 ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc
    11821 acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga
    11881 tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg
    11941 ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca
    12001 aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct
    12061 tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg
    12121 tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg
    12181 tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa
    12241 ctcggtttgt cgtctgttgc gggggcggca gttatggcg
//
