LOCUS       sequence-21885-e         247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: pET41a-MED18
FEATURES             Location/Qualifiers
     source          1..247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..60
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             76..120
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             151..165
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 actagtggtt ctggtcatca ccatcaccat cactccgcgg gtctggtgcc acgcggtagt
       61 actgcaattg gtatgaaaga aaccgctgct gctaaattcg aacgccagca catggacagc
      121 ccagatctgg gtaccggtgg tggctccggt gatgacgacg acaagagtcc catgggatat
      181 cggggatcat ggaggcacct ccagtcacca tgatgcctgt cactgggggc accattaaca
      241 tgatgga
//
