LOCUS       sequence-218851-       10186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  blue light inducible cryptochrome based optogenetic expression 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Harrigan P, Madhani HD, El-Samad H
  TITLE     Real-Time Genetic Compensation Defines the Dynamic Demands of 
            Feedback Control.
  JOURNAL   Cell. 2018 Oct 18;175(3):877-886.e10. doi: 
            10.1016/j.cell.2018.09.044.
  PUBMED    30340045
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09"; journalName: "Cell"; date: "2018-10-18- 
            18"; volume: "175"; issue: "3"; pages: "877-886.e10"
COMMENT     Sequence Label: pHES945
FEATURES             Location/Qualifiers
     source          1..10186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        123..766
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     659..682
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             780..1220
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1236..2729
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     primer_bind     complement(4017..4036)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4018..4036)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4043..4060)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4043..4059)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4052..4074)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4200..4655
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4287..4306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4497..4518
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5011..5030)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5130..5152
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5190..5208)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5293..5397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5398..5466,5467..6258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5616..5635)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6429..7017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6918..6937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7171..7188
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7305..7326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7341..7346,7347..7364,7365..7371)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7379..7395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7384..7406
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7403..7419
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7403..7419
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7440..7458
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7746..8153
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 gaatgttagc gtcaacaaca agaagtttaa tgacgcggag gccaaggcaa aaagattcct
       61 tgattacgta agggagttag aatcattttg aataaaaaac acgctttttc agttcgagtt
      121 tatcattatc aatactgcca tttcaaagaa tacgtaaata attaatagta gtgattttcc
      181 taactttatt tagtcaaaaa attagccttt taattctgct gtaacccgta catgcccaaa
      241 atagggggcg ggttacacag aatatataac atcgtaggtg tctgggtgaa cagtttattc
      301 ctggcatcca ctaaatataa tggagcccgc tttttaagct ggcatccaga aaaaaaaaga
      361 atcccagcac caaaatattg ttttcttcac caaccatcag ttcataggtc cattctctta
      421 gcgcaactac agagaacagg ggcacaaaca ggcaaaaaac gggcacaacc tcaatggagt
      481 gatgcaacct gcctggagta aatgatgaca caaggcaatt gacccacgca tgtatctatc
      541 tcattttctt acaccttcta ttaccttctg ctctctctga tttggaaaaa gctgaaaaaa
      601 aaggttgaaa ccagttccct gaaattattc ccctacttga ctaataagta tataaagacg
      661 gtaggtattg attgtaattc tgtaaatcta tttcttaaac ttcttaaatt ctacttttat
      721 agttagtctt ttttttagtt ttaaaacacc aagaacttag tttcgaactc gacaataaaa
      781 tgaagctact gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt
      841 gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact
      901 ctcccaaaac caaaaggtct ccgctgacta gggcacatct gacagaagtg gaatcaaggc
      961 tagaaagact ggaacagcta tttctactga tttttcctcg agaagacctt gacatgattt
     1021 tgaaaatgga ttctttacag gatataaaag cattgttaac aggattattt gtacaagata
     1081 atgtgaataa agatgccgtc acagatagat tggcttcagt ggagactgat atgcctctaa
     1141 cattgagaca gcatagaata agtgcgacat catcatcgga agagagtagt aacaaaggtc
     1201 aaagacagtt gactgtatcg ccgctcgagg ccaccatgaa gatggacaaa aagactatag
     1261 tttggtttag aagagaccta aggattgagg ataatcctgc attagcagca gctgctcacg
     1321 aaggatctgt ttttcctgtc ttcatttggt gtcctgaaga agaaggacag ttttatcctg
     1381 gaagagcttc aagatggtgg atgaaacaat cacttgctca cttatctcaa tccttgaagg
     1441 ctcttggatc tgacctcact ttaatcaaaa cccacaacac gatttcagcg atcttggatt
     1501 gtatccgcgt taccggtgct acaaaagtcg tctttaacca cctctatgat cctgtttcgt
     1561 tagttcggga ccataccgta aaggagaagc tggtggaacg tgggatctct gtgcaaagct
     1621 acaatggaga tctattgtat gaaccgtggg agatatactg cgaaaagggc aaacctttta
     1681 cgagtttcaa ttcttactgg aagaaatgct tagatatgtc gattgaatcc gttatgcttc
     1741 ctcctccttg gcggttgatg ccaataactg cagcggctga agcgatttgg gcgtgttcga
     1801 ttgaagaact agggctggag aatgaggccg agaaaccgag caatgcgttg ttaactagag
     1861 cttggtctcc aggatggagc aatgctgata agttactaaa tgagttcatc gagaagcagt
     1921 tgatagatta tgcaaagaac agcaagaaag ttgttgggaa ttctacttca ctactttctc
     1981 cgtatctcca tttcggggaa ataagcgtca gacacgtttt ccagtgtgcc cggatgaaac
     2041 aaattatatg ggcaagagat aagaacagtg aaggagaaga aagtgcagat ctttttctta
     2101 ggggaatcgg tttaagagag tattctcggt atatatgttt caacttcccg tttactcacg
     2161 agcaatcgtt gttgagtcat cttcggtttt tcccttggga tgctgatgtt gataagttca
     2221 aggcctggag acaaggcagg accggttatc cgttggtgga tgccggaatg agagagcttt
     2281 gggctaccgg atggatgcat aacagaataa gagtgattgt ttcaagcttt gctgtgaagt
     2341 ttcttctcct tccatggaaa tggggaatga agtatttctg ggatacactt ttggatgctg
     2401 atttggaatg tgacatcctt ggctggcagt atatctctgg gagtatcccc gatggccacg
     2461 agcttgatcg cttggacaat cccgcgttac aaggcgccaa atatgaccca gaaggtgagt
     2521 acataaggca atggcttccc gagcttgcga gattgccaac tgaatggatc catcatccat
     2581 gggacgctcc tttaaccgta ctcaaagctt ctggtgtgga actcggaaca aactatgcga
     2641 aacccattgt agacatcgac acagctcgtg agctactagc taaagctatt tcaagaaccc
     2701 gtgaagcaca gatcatgatc ggagcagcat aataagcggc cgccaccgcg gtggagctct
     2761 aagcaaatag ctaaattata tacgaattaa tattatgatt aagtgtttac gtgagtgcga
     2821 tatttttatt actatcttat acagttgtat atactctata aaatgagttg tctattaatt
     2881 aacgcgatga atgctttctg ggtttacctc tccaacaact ctagtttact tctcaataca
     2941 ttcaattgta tttgatttgt caatacttca tcattaatca attctatagt tttgtttttc
     3001 tcgtttattt ccaaatttaa tgcatcaatt ttattattca atttgtcgtt gattttggtt
     3061 aatgatttta tggtttgatc tctggcattg attgtttgtg ttagtttttc attattgata
     3121 attaaattat ttaagttagt tatcaactcg gtgttttcaa gtttcaagtt ttcaatttct
     3181 ttagagttta ttagatttgt caaagtttct gaattgcttg attggtcctg tagaagagta
     3241 tttgttgttg tggataattg attcaatttt tgagacaatt gctggaaggc gttgaaatat
     3301 ctagcatcaa tctcatggtt tttttcccga gagtctcgta gattcaattg ttttaatata
     3361 tcttgggacc actcttgatt tgaactcatg gaaattaaac tgggtgttgt gttgtggtgt
     3421 aatgattgta ccccctttgc ttataattgt gtggccaccg aagtcggtga tgctgtcgcc
     3481 gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata
     3541 aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca
     3601 tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag
     3661 gatgtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat aaggaaaaag
     3721 aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa gttaggtgta
     3781 acagaaaatc atgaaactat gattcctaat ttatatattg gaggattttc tctaaaaaaa
     3841 aaaaaataca acaaataaaa aacactcaat gacctgacca tttgatggag tttaagtcaa
     3901 tacctccttg aaccatttcc cataatggtg aaagttccct caagaatttt actctgtcag
     3961 aaacggcctt aacgacgtag gccggccatt taaatgttta aacggccacg ctggccccct
     4021 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4081 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4141 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     4201 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4261 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4321 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4381 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4441 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4501 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4561 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4621 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
     4681 catctgtgcg gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct
     4741 aatttgtgag tttagtatta ggatctctac ccttggcgaa aagtcctctg ccaacaatga
     4801 tgatatctga tccaccactt acaacttcgt cgacggttct gtactgctga cccaatatgc
     4861 gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta
     4921 ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg
     4981 aattgaaaag gtggtatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     5041 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     5101 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     5161 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5221 tgtcatgata ataatggttt cttagacgtc ggcgcgccga ggacgtcagg tggcactttt
     5281 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5341 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5401 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5461 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5521 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5581 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5641 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5701 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5761 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5821 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5881 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5941 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6001 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6061 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6121 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6181 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6241 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6301 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6361 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6421 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6481 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6541 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6601 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6661 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6721 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6781 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6841 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6901 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6961 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7021 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7081 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7141 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7201 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7261 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7321 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7381 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgga
     7441 attaaccctc actaaaggga acaaaagctg ggccggccat ttaaatgttt aaacggccac
     7501 gctggccgag gagaacttct agtatatcta catacctaat attattgcct tattaaaaat
     7561 ggaatcccaa cgattacatc aaaatccaca ttctcttcaa aatcaattgt cctgtacttc
     7621 cttgttcatg tgtgttcaaa aacgttatat ttataggata attatactct atttctcaac
     7681 aagtaattgg ttgtttggcc gagcggtcta aggcgcctga ttcaagaaat atcttgaccg
     7741 cagttaactg tgggaatact caggtatcgt aagatgcaag agttcgaatc tcttagcaac
     7801 cattattttt ttcctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat
     7861 tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa
     7921 ctgaaaaatt gggagaaaaa ggaaaggtga gagcgccgga accggctttt catatagaat
     7981 agagaagcgt tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta
     8041 aggacctatt gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta
     8101 ccttttacat ttcagcaata tatatatata tatttcaagg atataccatt ctaatgtctg
     8161 cccctaagtt accaatagat agcggtttaa tataaggttt caacaagaaa tattttgtga
     8221 aaatcaaaag ttaatagttt cactgtcgaa aatatgaaag tttaagtttt cagttcatcc
     8281 cattgtagtt atcaaatttc tgtgtttccc gtatatgtgt gctctgtatt catcgctatc
     8341 ttgaaactgg ttatgatcgt tgcgcatgtg acctgtatcg ctcaaaagag cccggcaccg
     8401 gtaccgggca aaagccccta gcaagctaaa atgattcaac tttcgtagta atttgttcaa
     8461 ccggtcacag taaagtcaat gactaacttt tgaatcgagg atgtatttcc aattgaaagc
     8521 aatgtatata taaaccattg tgttgaagct tgtcttacag ttttcttgta tcctcccatt
     8581 gttcatatag tacttaaagt accataatcc ttaaagaaca aacttgaaag agaatacact
     8641 atacacaact aataaataaa gaataatcat ggctgtgacc aagacaattg tagttctacc
     8701 aggtgaccat gttggtcaag aaatcactga agaggccatt aaagttttaa atgctattca
     8761 ggaatgtcgt ccagacaagg tcaatttcaa gtttgatcat catttgatcg gtggtgctgc
     8821 aattgatgcc actggtgttc cattaccaga cgaagctttg gaggcctcta agaaagctga
     8881 tgccgtgctt ctgggtgctg ttggtggtcc aaaatggggt actggcgctg tcagaccaga
     8941 acaaggtctt ttgaaaatcc gtaaggagtt gcaattgtat gctaatctaa gaccatgtaa
     9001 ttttgcatct gattccttac tagatctatc gccattgaag cctgaaattg caagaggtac
     9061 agatttcgtc gttgttagag aactagtggg tggtatttat ttcggggaga gaaaagaaga
     9121 tgaaggtgat ggtgtcgcct gggatagcga aaagtattct gtgcccgaag ttcaaagaat
     9181 cactagaatg gctgctttca tggctctgca acacaaccca cctttaccta tttggtctct
     9241 agataaagcc aacgttctag catcatcaag attatggaga aaaacagtgg aagagacgat
     9301 taagaatgaa ttcccacaat tgactgttca acatcaattg attgattccg ctgctatgat
     9361 cttggttaag aacccaactc atctaaatgg tattattatc acaaacaata tgtttggtga
     9421 tattatctcc gatgaagcct ccgtgatccc aggttcgtta ggattacttc catctgcctc
     9481 cttggcatct cttccagata agaatacggc atttggttta tatgagccat gtcatggctc
     9541 tgctccagac ttaccaaagg gaaaggttaa ccccgtagca acaatcctat ctgctgctat
     9601 gatgttgaag ttgtctctgg acctgttcga agaaggtgaa attatagaac aagcagtcaa
     9661 gaaagtgttg gattctggta tcagaacagc ggatctaaag ggtaccaact ctactactga
     9721 ggtcggtgat gcagtagcga aagctgtcag ggaactatta gcttagactt agtagatgta
     9781 cagttgcata cataagtctg aacagaaaga agtaaatatc atatctagtt cggtttagaa
     9841 atcagtgact atattggttt tgaccagatc gcaatccttg caagcattta gtaatgacta
     9901 tcaaaccggt gaatcatctg ataaggctat attagcttgt gcattcgcat gtatcgggaa
     9961 acgaacggta ccgggccctt ttcgaggacc ttgtcacctt gagcccaaga gagccaagat
    10021 ttaaattttc ctatgacttg atgcaaattc ccaaagctaa taacatgcaa gacacgtacg
    10081 gtcaagaaga catatttgac ctcttaacag gttcagacgc gactgcctca tcagtaagac
    10141 ccgttgaaaa gaacttacct gaaaaaaacg aatatatact agcgtt
//
