LOCUS       sequence-218852-       10608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the hTert protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10608)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCLX-PGK-hTERT
FEATURES             Location/Qualifiers
     source          1..10608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        26..536
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     455..473
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    537..561
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             656..4054
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     protein_bind    complement(4066..4090)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4108..4696
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4161..4181)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4579..4590)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4839..4872)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4892..5072
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5110..5129)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5175..5230
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5229..5248)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5591..5607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5591..5607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5604..5626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5615..5631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5639..5645,5646..5663,5664..5669))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5684..5705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5764..5959
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5810..5945
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5965..6099
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6002..6021)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6056..6075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6167..6184)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6338..6926)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6418..6437)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7097..7888,7889..7957))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7720..7739
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7958..8062)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8093..8472
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8474..8731
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8778..8903
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9395..9512
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9872..10105
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10290..10334
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caactttgta tagaaaagtt gccacggggt tggggttgcg ccttttccaa ggcagccctg
       61 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
      121 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
      181 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
      241 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
      301 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
      361 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
      421 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
      481 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggcaa
      541 gtttgtacaa aaaagcaggc tactagtgag gaaccaattc agtcgactgg atccggtacc
      601 gaattaattc gtcctgctgc gcacgtggga agccctggcc ccggccaccc ccgcgatgcc
      661 gcgcgctccc cgctgccgag ccgtgcgctc cctgctgcgc agccactacc gcgaggtgct
      721 gccgctggcc acgttcgtgc ggcgcctggg gccccagggc tggcggctgg tgcagcgcgg
      781 ggacccggcg gctttccgcg cgctggtggc ccagtgcctg gtgtgcgtgc cctgggacgc
      841 acggccgccc cccgccgccc cctccttccg ccaggtgtcc tgcctgaagg agctggtggc
      901 ccgagtgctg cagaggctgt gcgagcgcgg cgcgaagaac gtgctggcct tcggcttcgc
      961 gctgctggac ggggcccgcg ggggcccccc cgaggccttc accaccagcg tgcgcagcta
     1021 cctgcccaac acggtgaccg acgcactgcg ggggagcggg gcgtgggggc tgctgctgcg
     1081 ccgcgtgggc gacgacgtgc tggttcacct gctggcacgc tgcgcgctct ttgtgctggt
     1141 ggctcccagc tgcgcctacc aggtgtgcgg gccgccgctg taccagctcg gcgctgccac
     1201 tcaggcccgg cccccgccac acgctagtgg accccgaagg cgtctgggat gcgaacgggc
     1261 ctggaaccat agcgtcaggg aggccggggt ccccctgggc ctgccagccc cgggtgcgag
     1321 gaggcgcggg ggcagtgcca gccgaagtct gccgttgccc aagaggccca ggcgtggcgc
     1381 tgcccctgag ccggagcgga cgcccgttgg gcaggggtcc tgggcccacc cgggcaggac
     1441 gcgtggaccg agtgaccgtg gtttctgtgt ggtgtcacct gccagacccg ccgaagaagc
     1501 cacctctttg gagggtgcgc tctctggcac gcgccactcc cacccatccg tgggccgcca
     1561 gcaccacgcg ggccccccat ccacatcgcg gccaccacgt ccctgggaca cgccttgtcc
     1621 cccggtgtac gccgagacca agcacttcct ctactcctca ggcgacaagg agcagctgcg
     1681 gccctccttc ctactcagct ctctgaggcc cagcctgact ggcgctcgga ggctcgtgga
     1741 gaccatcttt ctgggttcca ggccctggat gccagggact ccccgcaggt tgccccgcct
     1801 gccccagcgc tactggcaaa tgcggcccct gtttctggag ctgcttggga accacgcgca
     1861 gtgcccctac ggggtgctcc tcaagacgca ctgcccgctg cgagctgcgg tcaccccagc
     1921 agccggtgtc tgtgcccggg agaagcccca gggctctgtg gcggcccccg aggaggagga
     1981 cacagacccc cgtcgcctgg tgcagctgct ccgccagcac agcagcccct ggcaggtgta
     2041 cggcttcgtg cgggcctgcc tgcgccggct ggtgccccca ggcctctggg gctccaggca
     2101 caacgaacgc cgcttcctca ggaacaccaa gaagttcatc tccctgggga agcatgccaa
     2161 gctctcgctg caggagctga cgtggaagat gagcgtgcgg ggctgcgctt ggctgcgcag
     2221 gagcccaggg gttggctgtg ttccggccgc agagcaccgt ctgcgtgagg agatcctggc
     2281 caagttcctg cactggctga tgagtgtgta cgtcgtcgag ctgctcaggt ctttctttta
     2341 tgtcacggag accacgtttc aaaagaacag gctctttttc taccggaaga gtgtctggag
     2401 caagttgcaa agcattggaa tcagacagca cttgaagagg gtgcagctgc gggagctgtc
     2461 ggaagcagag gtcaggcagc atcgggaagc caggcccgcc ctgctgacgt ccagactccg
     2521 cttcatcccc aagcctgacg ggctgcggcc gattgtgaac atggactacg tcgtgggagc
     2581 cagaacgttc cgcagagaaa agagggccga gcgtctcacc tcgagggtga aggcactgtt
     2641 cagcgtgctc aactacgagc gggcgcggcg ccccggcctc ctgggcgcct ctgtgctggg
     2701 cctggacgat atccacaggg cctggcgcac cttcgtgctg cgtgtgcggg cccaggaccc
     2761 gccgcctgag ctgtactttg tcaaggtgga tgtgacgggc gcgtacgaca ccatccccca
     2821 ggacaggctc acggaggtca tcgccagcat catcaaaccc cagaacacgt actgcgtgcg
     2881 tcggtatgcc gtggtccaga aggccgccca tgggcacgtc cgcaaggcct tcaagagcca
     2941 cgtctctacc ttgacagacc tccagccgta catgcgacag ttcgtggctc acctgcagga
     3001 gaccagcccg ctgagggatg ccgtcgtcat cgagcagagc tcctccctga atgaggccag
     3061 cagtggcctc ttcgacgtct tcctacgctt catgtgccac cacgccgtgc gcatcagggg
     3121 caagtcctac gtccagtgcc aggggatccc gcagggctcc atcctctcca cgctgctctg
     3181 cagcctgtgc tacggcgaca tggagaacaa gctgtttgcg gggattcggc gggacgggct
     3241 gctcctgcgt ttggtggatg atttcttgtt ggtgacacct cacctcaccc acgcgaaaac
     3301 cttcctcagg accctggtcc gaggtgtccc tgagtatggc tgcgtggtga acttgcggaa
     3361 gacagtggtg aacttccctg tagaagacga ggccctgggt ggcacggctt ttgttcagat
     3421 gccggcccac ggcctattcc cctggtgcgg cctgctgctg gatacccgga ccctggaggt
     3481 gcagagcgac tactccagct atgcccggac ctccatcaga gccagtctca ccttcaaccg
     3541 cggcttcaag gctgggagga acatgcgtcg caaactcttt ggggtcttgc ggctgaagtg
     3601 tcacagcctg tttctggatt tgcaggtgaa cagcctccag acggtgtgca ccaacatcta
     3661 caagatcctc ctgctgcagg cgtacaggtt tcacgcatgt gtgctgcagc tcccatttca
     3721 tcagcaagtt tggaagaacc ccacattttt cctgcgcgtc atctctgaca cggcctccct
     3781 ctgctactcc atcctgaaag ccaagaacgc agggatgtcg ctgggggcca agggcgccgc
     3841 cggccctctg ccctccgagg ccgtgcagtg gctgtgccac caagcattcc tgctcaagct
     3901 gactcgacac cgtgtcacct acgtgccact cctggggtca ctcaggacag cccagacgca
     3961 gctgagtcgg aagctcccgg ggacgacgct gactgccctg gaggccgcag ccaacccggc
     4021 actgccctca gacttcaaga ccatcctgga ctgagtcgaa tctagaccca gctttcttgt
     4081 acaaagtggt gataatcgaa ttccgataat caacctctgg attacaaaat ttgtgaaaga
     4141 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4201 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4261 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4321 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4381 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4441 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4501 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4561 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4621 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4681 tgggccgcct ccccgcatcg ggaattcccg cggttcgctt taagaccaat gacttacaag
     4741 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     4801 tcccaacgaa gacaagatgg gatcaattca ccatgggaat aacttcgtat agcatacatt
     4861 atacgaagtt atgctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     4921 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4981 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5041 acccttttag tcagtgtgga aaatctctag cagcatctag ttgaatcgcc agtgtgtcga
     5101 gctagcagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     5161 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     5221 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     5281 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     5341 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     5401 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     5461 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     5521 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg
     5581 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5641 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5701 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     5761 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5821 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5881 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5941 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     6001 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6061 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
     6121 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6181 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6241 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6301 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6361 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6421 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6481 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6541 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6601 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6661 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6721 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6781 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6841 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6901 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6961 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7021 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7081 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7141 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7201 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7261 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7321 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7381 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7441 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7501 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7561 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7621 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7681 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7741 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7801 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7861 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7921 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7981 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8041 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     8101 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     8161 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     8221 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     8281 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     8341 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     8401 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     8461 gctattacca tgggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     8521 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     8581 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8641 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8701 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8761 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8821 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8881 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8941 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     9001 gcagggagct agaacgattc gcagttaata ctggcctgtt agaaacatca gaaggctgta
     9061 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     9121 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     9181 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaaaaaa gcacagcaag
     9241 cagcaggatc ttcagacctg gaaattccct acaatcccca aagtcaagga gtagtagaat
     9301 ctatgaataa agaattaaag aaaattatag gacaggtaag agatcaggct gaacatctta
     9361 agacagcagt acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     9421 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     9481 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagaaatc
     9541 cactttggaa aggaccagca aagctcctct ggaaaggtga aggggcagta gtaatacaag
     9601 ataatagtga cataaaagta gtgccaagaa gaaaagcaaa gatcattagg gattatggaa
     9661 aacagatggc aggtgatgat tgtgtggcaa gtagacagga tgaggattag aacatggaaa
     9721 agtttagtaa aacaccataa ggaggagata tgagggacaa ttggagaagt gaattatata
     9781 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9841 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9901 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9961 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    10021 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    10081 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    10141 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10201 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10261 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10321 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10381 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10441 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    10501 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
    10561 ccattcgatt agtgaacgga tctcgacggt atctagtgat tatcggat
//
