LOCUS       sequence-218855-        9142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control, silence GFP gene, doxycycline inducible, neomycin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Reis LMD, Adamoski D, Ornitz Oliveira Souza R, Rodrigues Ascencao 
            CF, Sousa de Oliveira KR, Correa-da-Silva F, Malta de Sa Patroni F, 
            Meira Dias M, Consonni SR, Mendes de Moraes-Vieira PM, Silber AM, 
            Dias SMG
  TITLE     Dual inhibition of glutaminase and carnitine palmitoyltransferase 
            decreases growth and migration of glutaminase inhibition-resistant 
            triple-negative breast cancer cells.
  JOURNAL   J Biol Chem. 2019 Jun 14;294(24):9342-9357. doi: 
            10.1074/jbc.RA119.008180. Epub 2019 Apr 30.
  PUBMED    31040181
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.008180"; journalName: "J Biol Chem"; date: 
            "2019-06-14- 14"; volume: "294"; issue: "24"; pages: "9342-9357"
COMMENT     Sequence Label: tet-pLKO.neo_shGFP
FEATURES             Location/Qualifiers
     source          1..9142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1824..1918
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     1835..1854
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    1872..1890
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1900..1918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2019..2136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2185..2695
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2614..2632
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          2704..3276
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3284..3303
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3331..3349
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3360..3980
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3433..3452)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4015..4588
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4182..4199)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4409..4428
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4589..5389
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4649..4668)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5259..5278
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(5418..5517)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             5661..5894
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5966..6087
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6003..6022)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6057..6076
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6127..6262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6283..6301)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6311..6328)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6311..6327)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6320..6342)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6469..6924
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6556..6575)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6766..6787
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6950..7054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7055..7123,7124..7915)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7273..7292)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8086..8674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8575..8594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8828..8845
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8962..8983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8998..9003,9004..9021,9022..9028)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9036..9052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9041..9063
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9060..9076
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9060..9076
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9095..9115
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9097..9115
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggcc gcaatatttg catgtcgcta tgtgttctgg gaaatcacca
     1861 taaacgtgaa atccctatca gtgatagaga cttataagtt ccctatcagt gatagagaca
     1921 ccggcaagct gaccctgaag ttcatctcga gatgaacttc agggtcagct tgtttttgaa
     1981 ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2041 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2101 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2161 gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg
     2221 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc
     2281 tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct
     2341 tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt
     2401 cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg
     2461 acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg
     2521 ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg
     2581 ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga
     2641 gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga
     2701 tctgtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta
     2761 tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac
     2821 catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct
     2881 cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa
     2941 cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt
     3001 ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt
     3061 gttataatta aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat
     3121 attcttattg gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc tttatggtta
     3181 caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct
     3241 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     3301 gtgctgtctc atcattttgg caaagaattg taatacgact cactataggg cgaattgata
     3361 tgtctagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
     3421 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
     3481 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag
     3541 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta
     3601 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt
     3661 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat
     3721 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta
     3781 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac
     3841 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag
     3901 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac
     3961 ttaaatgtga aagtgggtcc gcgtacagcg gctcccggga gttctaggga tctgcccctc
     4021 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     4081 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     4141 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     4201 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     4261 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     4321 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     4381 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     4441 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     4501 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     4561 ggttttcctt tgaaaaacac gatgataagg atccatgatt gaacaagatg gattgcacgc
     4621 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4681 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4741 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     4801 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4861 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4921 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4981 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5041 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5101 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5161 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5221 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5281 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5341 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag gtaccttttt
     5401 taagggtggg cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg
     5461 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     5521 tccagctgat atccgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     5581 ccatgcatcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5641 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5701 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5881 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     5941 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     6001 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6061 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     6121 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6181 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6241 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     6301 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6361 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6421 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     6481 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6541 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6601 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6661 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6721 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6781 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6841 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     6901 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6961 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7021 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7081 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7141 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7201 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7261 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7321 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7381 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7441 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7501 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7561 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7621 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7681 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7741 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7801 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7861 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7921 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7981 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8041 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8101 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8161 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8221 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8281 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8341 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8401 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8461 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8521 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8581 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8641 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8701 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8761 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8821 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     8881 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     8941 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9001 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9061 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     9121 aaagctggag ctgcaagctt aa
//
