LOCUS       sequence-218858-         598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse 
            Gata3 coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Gu B, Posfai E, Rossant J
  TITLE     Efficient generation of targeted large insertions by microinjection 
            into two-cell-stage mouse embryos.
  JOURNAL   Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166.
  PUBMED    29889212
REFERENCE   2  (bases 1 to 598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166."
COMMENT     Sequence Label: Gata3-T2A-H2B-eGFP donor
FEATURES             Location/Qualifiers
     source          1..598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catttaaaaa aaaaaaaaag aaagaaaaag aaaaaaaggg gggggggggg agaaaaaaat
       61 aaattaaaaa aaaaaaaaaa aaagaaaaga aagaaaaatt taagaaaaaa aaaaaaaagg
      121 ttgtaggcaa atcatttgtt ccaggctgtg agcctgtgca aaagagattt cagatctggg
      181 caatgggtgt gtgatctcac ccactgaaga tttgagaatg tcatggctag gcctacatgc
      241 tctgtgaatc agtccctgta attgttgttt gtatgtataa ttcagaagca ccaaaataag
      301 aaaagatgta gatttatttc atcatattat acagactgaa ttgttgtata aatttattta
      361 ctgctagtgt taggaactgc tttttttttt ttttttggtt ttaatgtttt tttttttttt
      421 gttttttgtt tttttttttc tttctctctg gatttttggt tgaataaact agattgcttt
      481 cagttgactt aaggtggatg tactctggag ggtttatttt tccttttatt attatttttg
      541 atggtattta ttaaatagct tctatgggcc cggcggtacc tgtctttttc gtcacttt
//
