LOCUS       sequence-218865-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An HRas biosensor based on fluorescence resonance energy transfer 
            (FRET) and those based on bioluminescence energy transfer (BRET).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada 
            Y, Nagai T, Matsuda M
  TITLE     A platform of BRET-FRET hybrid biosensors for optogenetics, chemical
            screening, and in vivo imaging.
  JOURNAL   Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.1038/s41598-018-27174-x.
  PUBMED    29895862
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-12"; volume: "8(1):8984. doi"; pages: " 
            10.1038/s41598-018-27174-x"
COMMENT     Sequence Label: pCSIIbsr-6013x
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(278..625)
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
ORIGIN
        1 gctcctcgcc cttgctcacc atgcggccgc ccaggaaatc tacgtgaagt tcttctccaa
       61 tcaaagacgc agcttcagta ttccaatcta agcgtgcttt tttacctttg tgttcgtgga
      121 gaagtctgaa cactgcacag ctctctggtt gcaggcccct caccttgagt gctttcataa
      181 ggcagtcatg caagctcatt ccatttcgca cattgaccac tgttctttgc ttgttcggca
      241 agaaaacacg gatagtgttg cttgtcttag aaggggtacc accagcacta ccaccagcac
      301 taccaccagc actaccacca gcactaccac cagcactggt actaccacca gcactaccac
      361 cagcactgcc ggaaccacca gcactaccac cagcactacc accagcacta ccaccagcac
      421 taccaccagc actggtacta ccaccagcac taccaccagc actgccggaa ccaccagcac
      481 taccaccagc actaccacca gcactaccac cagcactacc accagcactg gtactaccac
      541 cagcactacc accagcactg ccggaaccac cagcactacc accagcacta ccaccagcac
      601 taccaccagc actaccacca gcactggttc ctccggagcc gttcagcttc cgcagcttgt
      661 gctgccggat ctcacgcacc aacgtgtaga aggcatcctc cactccctgc cgggtcttgg
      721 ccgaggtctc gatgtagggg atgccgtagc ttcgggcgag gtcctgagcc tgccgagatt
      781 ccacagtgcg tgcagccagg tcacacttgt tccccaccag caccatgggc acgtcatccg
      841 agtccttcac ccgtttgatc tgctccctg
//
