LOCUS       sequence-218867-        8824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific expression of the GFP living color under the control
            of the Keratin7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8824)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 8824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCLX-KRT7-GFP
FEATURES             Location/Qualifiers
     source          1..8824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1528..1552
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1556..1565
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1562..1564,1565..1567,1568..2281)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1562..1564,1565..1567,1568..2278)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1607..1628)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1868..1887)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2215..2236
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2282..2306)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2324..2912
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2377..2397)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2795..2806)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3055..3088)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             3108..3288
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3326..3345)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3391..3446
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3445..3464)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3807..3823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3807..3823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3820..3842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3831..3847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3855..3861,3862..3879,3880..3885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3900..3921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3980..4175
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4026..4161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4181..4315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4218..4237)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4272..4291
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4383..4400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4554..5142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4634..4653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5313..6104,6105..6173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5936..5955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6174..6278)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6309..6688
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             6690..6947
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6994..7119
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7611..7728
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8088..8321
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8506..8550
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caactttgta tagaaaagtt ggctagcact ggagttttaa agaatgttcc agggaggggt
       61 aagaggccca gggcagatgt ggggtaggct caggaatgag gaagtatttc tcaagcctgt
      121 gtgtggtggt tatagggggc cgaggcctgg actgaagtgc ccaggtactt ttgtctgtgt
      181 gcttcccagc tcttgttaga ccctcatcct gaaggagctg tggctgaggg ggccaggacc
      241 cgtccaggtc agcgcactag ctgtgccctg ctggggctca gcttgagaaa gagaaggagc
      301 tcttggtgtg ggtgagaaac aacctccaaa gtgggggatg ggtggcaggg gctgtagcag
      361 gatggaaagt ggggtgcaga gggggacaaa ggactgagga agcaccttga ggggtaggga
      421 gtctccttga gagcttaggg ttgtctacct gaaaaggcaa agcaggaaga atatgacatt
      481 cccatcctcc ccacctagga cgcccctaaa ggaccagtct cactgcatgg agccagaggt
      541 gggctaaagg ctgtccaagt gagtctggga aaactggaag tccaggagca ctccgaggag
      601 cactgagcac accaccacgg gttaccacag aggccagttt tagttgattc ccttgtcagg
      661 catagcctag tgggtttgta agcaggaaaa atgaggcaga gcagggagga acgtctggat
      721 gtgaacggga gacaggacac acacatgtgc atacaaatgt gttcagttgt gaatccagac
      781 cccccatgca cagactgcac tcatatacac tcacactagt gtgttagggg tatgagggca
      841 cagaagggta cgcacagcca tcccaggaac ccatacagta gtttgaggag ggggtgaggc
      901 aagccctctg aacttggatg gtggggaggg cgcagttact gaaacagctc ttcccagtaa
      961 ggatcgtcac agggccaggt tactcaagaa ctgagctgag ctctccagta gcagctgggc
     1021 caggaaggag agtcctagga agcctagggc ttccccagcc agtgggtgag gggtttcaac
     1081 tttcagcagg agctaaagtt tgtcaggcac ttccttttgt agcacttggc tttcctactg
     1141 gcctcaggaa tctaggtatt cactcctcat acctgctcta ggaagccttc tcctgccaat
     1201 tgtaaggtgg tctctttgcg gtccagagat ttctgggctg tgggtatctt ggacaacttg
     1261 cctgaggtgt gggtgtctca cgacggcgtc tcggcctcct gcagacactt cccgagtgga
     1321 cgaagagcct tgggcggcgg ctgggggcgg tgcgagggca gaaggcaggc ggacaggaag
     1381 gccctggctg cagagaaagg tggccagcgg ccagccccgc cctgtcccca cctgtgtgcg
     1441 ctcagcctcc gccccgcatg gaggaataaa aggtcgcaga ctgcctgagg tcagggagtt
     1501 cgcgctgctc ctcgcctcgt ccagccagca agtttgtaca aaaaagcagg ctcccgccac
     1561 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1621 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1681 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1741 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1801 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1861 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1921 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1981 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     2041 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2101 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2161 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2221 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     2281 agacccagtt tcttgtacaa agtggtgata atcgaattcc gataatcaac ctctggatta
     2341 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2401 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2461 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2521 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2581 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     2641 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     2701 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     2761 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     2821 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     2881 gagtcggatc tccctttggg ccgcctcccc gcatcgggaa ttcccgcggt tcgctttaag
     2941 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     3001 ggaagggcta attcactccc aacgaagaca agatgggatc aattcaccat gggaataact
     3061 tcgtatagca tacattatac gaagttatgc tgctttttgc ttgtactggg tctctctggt
     3121 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3181 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3241 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagttga
     3301 atcgccagtg tgtcgagcta gcagatcttt ttccctctgc caaaaattat ggggacatca
     3361 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     3421 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     3481 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     3541 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     3601 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     3661 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     3721 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc
     3781 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     3841 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     3901 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     3961 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     4021 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4081 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     4141 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     4201 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4261 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     4321 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4381 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4441 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4501 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4561 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4621 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4681 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4741 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4801 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4861 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4921 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4981 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5041 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5101 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5161 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5221 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5281 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5341 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5401 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5461 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5521 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5581 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5641 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5701 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5761 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5821 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5881 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5941 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6001 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6061 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6241 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6301 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     6361 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     6421 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     6481 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     6541 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     6601 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     6661 ctacgtatta gtcatcgcta ttaccatggg aggcgtggcc tgggcgggac tggggagtgg
     6721 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     6781 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6841 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6901 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     6961 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7021 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7081 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7141 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7201 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatactgg cctgttagaa
     7261 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7321 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7381 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     7441 aaaaaagcac agcaagcagc aggatcttca gacctggaaa ttccctacaa tccccaaagt
     7501 caaggagtag tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat
     7561 caggctgaac atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga
     7621 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     7681 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     7741 agggacagca gaaatccact ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg
     7801 gcagtagtaa tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc
     7861 attagggatt atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag
     7921 gattagaaca tggaaaagtt tagtaaaaca ccataaggag gagatatgag ggacaattgg
     7981 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     8041 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     8101 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     8161 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     8221 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     8281 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     8341 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     8401 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     8461 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     8521 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     8581 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     8641 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     8701 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     8761 agagacagag acagatccat tcgattagtg aacggatctc gacggtatct agtgattatc
     8821 ggat
//
