LOCUS       sequence-218869-        8861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  eFFly luciferase for invivo Imaging, eGFP for flourescent detection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Sandhofer N, Metzeler KH, Rothenberg M, Herold T, Tiedt S, Groiss V,
            Carlet M, Walter G, Hinrichsen T, Wachter O, Grunert M, Schneider S,
            Subklewe M, Dufour A, Frohling S, Klein HG, Hiddemann W, Jeremias I,
            Spiekermann K
  TITLE     Dual PI3K/mTOR inhibition shows antileukemic activity in 
            MLL-rearranged acute myeloid leukemia.
  JOURNAL   Leukemia. 2015 Apr;29(4):828-38. doi: 10.1038/leu.2014.305. Epub 
            2014 Oct 17.
  PUBMED    25322685
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/leu.2014.305"; journalName: "Leukemia"; date: "2015-04"; 
            volume: "29"; issue: "4"; pages: "828-38"
COMMENT     Sequence Label: pCDH-EF1a-eFFly-eGFP
FEATURES             Location/Qualifiers
     source          1..8861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    27..143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        186..397
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             410..678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2374..2427
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2434..2436,2437..2439,2440..3153)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2434..2436,2437..2439,2440..3150)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2479..2500)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2740..2759)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3087..3108
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3160..3748
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3213..3233)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3631..3642)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3822..4055
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4127..4248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4164..4183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4218..4237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4288..4423
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4456..4472)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4456..4472)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4469..4491)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4480..4496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4504..4510,4511..4528,4529..4534))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4549..4570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4687..4704)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4858..5446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(5617..6408,6409..6477))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6240..6259
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6478..6582)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6650..6668
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6706..6728)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6828..6847
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7041..7063
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7055..7072
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7056..7072
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7087..7313
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7314..7494
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7541..7666
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8159..8392
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8577..8621
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
       61 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      121 caaattacaa aattcaaaat tttatcgata ctagtggatc tgcgatcgct ccggtgcccg
      181 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
      241 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
      301 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
      361 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
      421 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
      481 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
      541 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
      601 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
      661 tctgcgccgt tacagatcca agctgtgacc ggcgcctact ctagagctag cgaattcatg
      721 gaagatgcca agaacatcaa gaaaggccct gcccccttct accccctgga agatggcaca
      781 gccggcgagc agctgcacaa ggccatgaag agatacgccc tggtgcccgg caccatcgcc
      841 ttcaccgacg cccacatcga ggtggacatc acctacgccg agtatttcga gatgagcgtg
      901 cggctggccg aggccatgaa acgctacggc ctgaacacca accaccggat cgtggtgtgc
      961 agcgagaaca gcctgcagtt cttcatgccc gtgctgggcg ccctgttcat cggcgtggcc
     1021 gtggcccctg ccaacgacat ctacaacgag cgggagctgc tgaacagcat gggcatcagc
     1081 cagcccaccg tggtgttcgt gagcaagaag ggcctgcaga aaatcctgaa cgtgcagaag
     1141 aagctgccca tcatccagaa aatcatcatc atggacagca agaccgacta ccagggcttc
     1201 cagagcatgt acaccttcgt gaccagccac ctgccccctg gcttcaacga gtacgacttc
     1261 gtgcccgaga gcttcgaccg ggacaagacc atcgccctga tcatgaacag cagcggcagc
     1321 accggcctgc ctaaaggcgt ggccctgcct caccggaccg cctgcgtgcg gttcagccac
     1381 gcccgggacc ccatcttcgg caaccagatc atccccgaca ccgccatcct gagcgtggtg
     1441 cccttccacc acggcttcgg catgttcacc accctgggct acctgatctg cggcttccgg
     1501 gtggtgctga tgtaccggtt cgaggaagag ctgttcctgc ggagcctgca ggactacaag
     1561 atccagagcg ccctgctggt gcccaccctg ttcagctttt tcgccaagag caccctgatc
     1621 gacaagtacg acctgagcaa cctgcacgag atcgccagcg gcggagcccc cctgtccaaa
     1681 gaagtgggcg aagccgtcgc caagcggttc cacctgcccg gcatccggca gggctatggc
     1741 ctgaccgaga ccacaagcgc cattctgatc acccccgagg gcgacgacaa gcctggcgcc
     1801 gtgggcaagg tggtgccttt cttcgaggcc aaggtggtgg acctggacac cggcaagacc
     1861 ctgggcgtga accagcgggg cgagctgtgc gtgaggggcc ccatgatcat gagcggctac
     1921 gtgaacaacc ccgaggccac caacgccctg attgacaagg acggctggct gcacagcggc
     1981 gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg
     2041 atcaagtaca agggctacca ggtggcccca gccgagctgg aaagcatcct gctgcagcac
     2101 cccaacatct tcgatgccgg ggtggccgga ctgcccgacg acgatgccgg cgagctgcct
     2161 gccgccgtgg tggtgctgga acacggcaaa accatgaccg agaaagaaat cgtggactac
     2221 gtggccagcc aggtgaccac cgccaagaaa ctgagaggcg gcgtggtgtt tgtggacgag
     2281 gtgcccaagg gcctgacagg caagctggac gcccggaaga tccgggagat cctgatcaag
     2341 gccaagaagg gcggcaaggg atccgcggcc gctgagggca gaggaagtct tctaacatgc
     2401 ggtgacgtgg aggagaatcc cggccctatg catatggtga gcaagggcga ggagctgttc
     2461 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2521 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2581 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     2641 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2701 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2761 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2821 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2881 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     2941 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     3001 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     3061 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3121 atcactctcg gcatggacga gctgtacaag taagtcgaca atcaacctct ggattacaaa
     3181 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3241 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3301 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3361 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3421 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3481 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3541 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3601 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3661 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3721 cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     3781 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     3841 ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     3901 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     3961 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     4021 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     4081 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     4141 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4201 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     4261 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4321 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4381 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     4441 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4501 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4561 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4621 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4681 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4741 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6601 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     6661 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     6721 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     6781 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     6841 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     6901 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     6961 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     7021 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
     7081 gacgcgtgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     7141 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     7201 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     7261 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     7321 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7381 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7441 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     7501 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     7561 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     7621 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     7681 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     7741 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     7801 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     7861 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     7921 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     7981 aagtaagacc accgcacagc aagcggccac tgatcttcag acctggagga ggagatatga
     8041 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     8101 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     8161 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     8221 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     8281 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     8341 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     8401 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     8461 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     8521 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8581 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     8641 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     8701 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     8761 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     8821 aaggtggaga gagagacaga gacagatcca ttcgattagt g
//
