LOCUS       sequence-218871-       11130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the hM3Dq protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De 
            Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ
  TITLE     Perturbed Wnt signaling leads to neuronal migration delay, altered 
            interhemispheric connections and impaired social behavior.
  JOURNAL   Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w.
  PUBMED    29079819
REFERENCE   2  (bases 1 to 11130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-27"; volume: "8(1):1158. doi"; pages: " 
            10.1038/s41467-017-01046-w"
COMMENT     Sequence Label: pCLXEBR-pTF-hM3Dq
FEATURES             Location/Qualifiers
     source          1..11130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             63..89
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(1886..1910)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1947..1963
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1948..1964
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1979..2096
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2153..2364
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2383..2778
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2788..2847
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             3910..4281
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    4307..4895
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4360..4380)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4778..4789)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5037..5070)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5090..5270
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5308..5327)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5373..5428
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5427..5446)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5789..5805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5789..5805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5802..5824)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5813..5829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5837..5843,5844..5861,5862..5867))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5882..5903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5962..6157
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6008..6143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6163..6297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6200..6219)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6254..6273
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6365..6382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6536..7124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6616..6635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7295..8086,8087..8155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7918..7937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8156..8260)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8291..8670
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8672..8929
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8976..9101
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9593..9710
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10070..10303
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10488..10532
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    10823..10841
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    10864..10882
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    11028..11046
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 acaagtttgt acaaaaaagc aggctggcgc cggaaccaat tcagtcgact ggatccacca
       61 tgtacccata cgatgttcca gattacgcta tgaccttgca caataacagt acaacctcgc
      121 ctttgtttcc aaacatcagc tcctcctgga tacacagccc ctccgatgca gggctgcccc
      181 cgggaaccgt cactcatttc ggcagctaca atgtttctcg agcagctggc aatttctcct
      241 ctccagacgg taccaccgat gaccctctgg gaggtcatac cgtctggcaa gtggtcttca
      301 tcgctttctt aacgggcatc ctggccttgg tgaccatcat cggcaacatc ctggtaattg
      361 tgtcatttaa ggtcaacaag cagctgaaga cggtcaacaa ctacttcctc ttaagcctgg
      421 cctgtgccga tctgattatc ggggtcattt caatgaatct gtttacgacc tacatcatca
      481 tgaatcgatg ggccttaggg aacttggcct gtgacctctg gcttgccatt gactgcgtag
      541 ccagcaatgc ctctgttatg aatcttctgg tcatcagctt tgacagatac ttttccatca
      601 cgaggccgct cacgtaccga gccaaacgaa caacaaagag agccggtgtg atgatcggtc
      661 tggcttgggt catctccttt gtcctttggg ctcctgccat cttgttctgg caatactttg
      721 ttggaaagag aactgtgcct ccgggagagt gcttcattca gttcctcagt gagcccacca
      781 ttacttttgg cacagccatc gctggttttt atatgcctgt caccattatg actattttat
      841 actggaggat ctataaggaa actgaaaagc gtaccaaaga gcttgctggc ctgcaagcct
      901 ctgggacaga ggcagagaca gaaaactttg tccaccccac gggcagttct cgaagctgca
      961 gcagttacga acttcaacag caaagcatga aacgctccaa caggaggaag tatggccgct
     1021 gccacttctg gttcacaacc aagagctgga aacccagctc cgagcagatg gaccaagacc
     1081 acagcagcag tgacagttgg aacaacaatg atgctgctgc ctccctggag aactccgcct
     1141 cctccgacga ggaggacatt ggctccgaga cgagagccat ctactccatc gtgctcaagc
     1201 ttccgggtca cagcaccatc ctcaactcca ccaagttacc ctcatcggac aacctgcagg
     1261 tgcctgagga ggagctgggg atggtggact tggagaggaa agccgacaag ctgcaggccc
     1321 agaagagcgt ggacgatgga ggcagttttc caaaaagctt ctccaagctt cccatccagc
     1381 tagagtcagc cgtggacaca gctaagactt ctgacgtcaa ctcctcagtg ggtaagagca
     1441 cggccactct acctctgtcc ttcaaggaag ccactctggc caagaggttt gctctgaaga
     1501 ccagaagtca gatcactaag cggaaaagga tgtccctggt caaggagaag aaagcggccc
     1561 agaccctcag tgcgatcttg cttgccttca tcatcacttg gaccccatac aacatcatgg
     1621 ttctggtgaa caccttttgt gacagctgca tacccaaaac cttttggaat ctgggctact
     1681 ggctgtgcta catcaacagc accgtgaacc ccgtgtgcta tgctctgtgc aacaaaacat
     1741 tcagaaccac tttcaagatg ctgctgctgt gccagtgtga caaaaaaaag aggcgcaagc
     1801 agcagtacca gcagagacag tcggtcattt ttcacaagcg cgcacccgag caggccttgt
     1861 aggcggccgc actcgagata tctagaccca gctttcttgt acaaagtggt gattcgacct
     1921 gcaggcatgc aagctcgggc cccccctcga ggtcgacggt atcgataagc ttgataactt
     1981 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2041 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     2101 atcacgagac tagcctcgag aagcttgatc gatggctccg gtgcccgtca gtgggcagag
     2161 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     2221 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2281 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2341 aacgggtttg ccgccagaac acaggtgtcg tgacgcggat ccatggccaa gcctttgtct
     2401 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     2461 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     2521 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     2581 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     2641 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     2701 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     2761 ggttatgtgt gggagggcgg atcaggtcaa tgtactaact acgctttgtt gaagcttgcc
     2821 ggcgatgttg aaagtaaccc cggtcctatg tccagactgg acaagagcaa agtcataaac
     2881 ggcgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct
     2941 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
     3001 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa
     3061 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cattccgctg tgctctcctc
     3121 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
     3181 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
     3241 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
     3301 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga
     3361 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
     3421 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc ggcctcgagc
     3481 gaattccagt acctgccaga tacagacgat cgtcaccgga ttgaggagaa acgtaaaagg
     3541 acatatgaga ccttcaagag catcatgaag aagagtcctt tcagcggacc caccgacccc
     3601 cggcctccac ctcgacgcat tgctgtgcct tcccgcagct cagcttctgt ccccaagcca
     3661 gcaccccagc cctatccctt tacgtcatcc ctgagcacca tcaactatga tgagtttccc
     3721 accatggtgt ttccttctgg gcagatcagc caggcctcgg ccttggcccc ggcccctccc
     3781 caagtcctgc cccaggctcc agcccctgcc cctgctccag ccatggtatc agctctggcc
     3841 caggccccag cccctgtccc agtcctagcc ccaggccctc ctcaggctgt ggccccacct
     3901 gcccccaagc ccacccaggc tggggaagga acgctgtcag aggccctgct gcagctgcag
     3961 tttgatgatg aagacctggg ggccttgctt ggcaacagca cagacccagc tgtgttcaca
     4021 gacctggcat ccgtcgacaa ctccgagttt cagcagctgc tgaaccaggg catacctgtg
     4081 gccccccaca caactgagcc catgctgatg gagtaccctg aggctataac tcgcctagtg
     4141 acaggggccc agaggccccc cgacccagct cctgctccac tgggggcccc ggggctcccc
     4201 aatggcctcc tttcaggaga tgaagacttc tcctccattg cggacatgga cttctcagcc
     4261 ctgctgagtc agatcagctc ctaaagatcc gcgtcgtacg gtcgacaatc aacctctgga
     4321 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     4381 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4441 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4501 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4561 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4621 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4681 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     4741 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4801 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     4861 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta
     4921 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4981 ctggaagggc taattcactc ccaacgaaga caagatggga tcaattcacc atgggaataa
     5041 cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg ggtctctctg
     5101 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5161 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5221 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagtt
     5281 gaatcgccag tgtgtcgagc tagcagatct ttttccctct gccaaaaatt atggggacat
     5341 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     5401 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5461 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     5521 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     5581 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     5641 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     5701 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     5761 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5821 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5881 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5941 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6001 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6061 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6121 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     6181 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6241 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     6301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6961 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8281 cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     8341 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     8401 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     8461 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     8521 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     8581 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     8641 atctacgtat tagtcatcgc tattaccatg ggaggcgtgg cctgggcggg actggggagt
     8701 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     8761 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8821 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8881 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8941 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     9001 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     9061 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     9121 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     9181 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatact ggcctgttag
     9241 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     9301 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     9361 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     9421 agaaaaaagc acagcaagca gcaggatctt cagacctgga aattccctac aatccccaaa
     9481 gtcaaggagt agtagaatct atgaataaag aattaaagaa aattatagga caggtaagag
     9541 atcaggctga acatcttaag acagcagtac aaatggcagt attcatccac aattttaaaa
     9601 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9661 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9721 acagggacag cagaaatcca ctttggaaag gaccagcaaa gctcctctgg aaaggtgaag
     9781 gggcagtagt aatacaagat aatagtgaca taaaagtagt gccaagaaga aaagcaaaga
     9841 tcattaggga ttatggaaaa cagatggcag gtgatgattg tgtggcaagt agacaggatg
     9901 aggattagaa catggaaaag tttagtaaaa caccataagg aggagatatg agggacaatt
     9961 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    10021 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    10081 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    10141 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    10201 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    10261 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    10321 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    10381 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    10441 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    10501 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    10561 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    10621 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    10681 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
    10741 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttcggta
    10801 caactcccac tagacatatc ctactctatc attgatagag tgacccttcc atagacatat
    10861 cctactctat cattgataga gtgacccttc cactaggttc acttttctct atcattgata
    10921 gggagtggta aacaattgat ggacaaaatc tatcaatgat agagagtgca tgttactagt
    10981 attgttcttc tctatcattg atagtgactt tgcctaatca cttagcaact ctatcattga
    11041 tagagttcta gtattagcaa ggcctttcct ctatataagg aagttcattt catttggaga
    11101 agatccccgg ggatcctcta gagtcgaatc
//
