LOCUS       sequence-21888-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: pET41a-MED31
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..73
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             89..133
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             164..178
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             645..668
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(731..749)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      745..792
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aatcggatgg ttcactagtg gttctggtca tcaccatcac catcactccg cgggtctggt
       61 gccacgcggt agtactgcaa ttggtatgaa agaaaccgct gctgctaaat tcgaacgcca
      121 gcacatggac agcccagatc tgggtaccgg tggtggctcc ggtgatgacg acgacaagag
      181 tcccatgccg ctcgagatgg ccgcggccgt cgctatggag acagatgatg ctggaaatcg
      241 gcttcggttt cagttggagt tggaatttgt gcaatgttta gccaacccaa actaccttaa
      301 ttttcttgcc caaagaggtt acttcaaaga caaagctttt gttaattatc ttaagtattt
      361 gctttactgg aaagaaccag aatatgccaa atatctaaag taccctcagt gcttacatat
      421 gttagagctg ctccagtatg agcactttcg caaggagctg gtgaatgcgc agtgtgcgaa
      481 gttcatcgac gagcagcaga ttctgcactg gcagcactac tctcggaagc gggtgcgtct
      541 tcagcaagcc ctggccgagc agcagcagca gaataacaca gcagggaaat gaggatccga
      601 attctgtaca ggccttggcg cgcctgcagg cgagctccgt cgagcaccac caccaccacc
      661 accaccacta attgattaat acctaggctg ctaaacaaag cccgaaagga agctgagttg
      721 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      781 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
      841 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      901 gcgccctagc gcccgctcct ttcgctttct tccttccttt ctcgccacgt tcgccg
//
