LOCUS       sequence-21889-e         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, 
            Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW
  TITLE     Identification of mammalian Mediator subunits with similarities to 
            yeast Mediator subunits Srb5, Srb6, Med11, and Rox3.
  JOURNAL   J Biol Chem. 2003 Apr 25. 278(17):15123-7.
  PUBMED    12584197
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Apr 25. 278(17):15123-7."
COMMENT     Sequence Label: pET32b-MED19
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..367
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             389..406
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             416..433
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             440..484
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             500..514
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 tctagaatat tttgtttaac tttaagaagg agatatacat atgagcgata aaattattca
       61 cctgactgac gacagttttg acacggatgt actcaaagcg gacggggcga tcctcgtcga
      121 tttctgggca gagtggtgcg gtccgtgcaa aatgatcgcc ccgattctgg atgaaatcgc
      181 tgacgaatat cagggcaaac tgaccgttgc aaaactgaac atcgatcaaa accctggcac
      241 tgcgccgaaa tatggcatcc gtggtatccc gactctgctg ctgttcaaaa acggtgaagt
      301 ggcggcaacc aaagtgggtg cactgtctaa aggtcagttg aaagagttcc tcgacgctaa
      361 cctggccggt tctggttctg gccatatgca ccatcatcat catcattctt ctggtctggt
      421 gccacgcggt tctggtatga aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      481 cagcccagat ctgggtaccg acgacgacga caaggccatg gctgatatcg gatctaccat
      541 ggagaacttc acggcactgt ttggagctca gactgacccc ccaccgccac caagcgccct
      601 aggctttggg cctggaaagc cacccccgcc accccctcct cctcc
//
