LOCUS       sequence-218907-       10981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the Kir2.1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10981)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-Kir2.1
FEATURES             Location/Qualifiers
     source          1..10981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    165..183
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    245..269
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      338..347
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1559..1583)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1600..2110
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2029..2047
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      2126..2135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2132..2134,2135..2137,2138..2848)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2132..2134,2135..2137,2138..2848)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2177..2198)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2438..2457)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2785..2806
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2864..2923
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             3992..4363
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    4389..4977
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4442..4462)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4860..4871)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4980..4996)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4981..4997)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(5125..5158)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5178..5358
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5396..5415)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5461..5516
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5515..5534)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5877..5893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5877..5893)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5890..5912)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5901..5917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5925..5931,5932..5949,5950..5955))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5970..5991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6050..6245
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6096..6231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6251..6385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6288..6307)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6342..6361
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6453..6470)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6624..7211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6704..6723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7382..8173,8174..8242))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8005..8024
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8243..8347)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8378..8757
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8759..9016
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9063..9188
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9680..9797
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10157..10390
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10575..10619
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    10894..10914
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    10941..10959
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 actctatcat tgatagagtg acccttccac taggttcact tttctctatc attgataggg
       61 agtggtaaac aattgatgga caaaatctat caatgataga gagtgcatgt tactagtatt
      121 gttcttctct atcattgata gtgactttgc ctaatcactt agcaactcta tcattgatag
      181 agttctagta ttagcaaggc ctttcctcta tataaggaag ttcatttcat ttggagaaga
      241 tcccccaagt ttgtacaaaa aagcaggctt caccatgggc agtgtgagaa ccaaccgcta
      301 cagcatcgtc tcttcagaag aagacggtat gaagttggcc accatggcag ttgcaaatgg
      361 ctttgggaac gggaagagta aagtccacac ccgacaacag tgcaggagcc gctttgtgaa
      421 gaaagatggc cactgtaatg ttcagttcat caatgtgggt gagaaggggc aacggtacct
      481 cgcagacatc ttcaccacgt gtgtggacat tcgctggcgg tggatgctgg ttatcttctg
      541 cctggctttc gtcctgtcat ggctgttttt tggctgtgtg ttttggttga tagctctgct
      601 ccatggggac ctggatgcat ccaaagaggg caaagcttgt gtgtccgagg tcaacagctt
      661 cacggctgcc ttcctcttct ccattgagac ccagacaacc ataggctatg gtttcagatg
      721 tgtcacggat gaatgcccaa ttgctgtttt catggtggtg ttccagtcaa tcgtgggctg
      781 catcatcgat gctttcatca ttggcgcagt catggccaag atggcaaagc caaagaagag
      841 aaacgagact cttgtcttca gtcacaatgc cgtgattgcc atgagagacg gcaagctgtg
      901 tttgatgtgg cgagtgggca atcttcggaa aagccacttg gtggaagctc atgttcgagc
      961 acagctcctc aaatccagaa ttacttctga aggggagtat atccctctgg atcaaataga
     1021 catcaatgtt gggtttgaca gtggaatcga tcgtatattt ctggtgtccc caatcactat
     1081 agtccatgaa atagatgaag acagtccttt atatgatttg agtaaacagg acattgacaa
     1141 cgcagacttt gaaatcgtgg tcatactgga aggcatggtg gaagccactg ccatgacgac
     1201 acagtgccgt agctcttatc tagcaaatga aatcctgtgg ggccaccgct atgagcctgt
     1261 gctctttgaa gagaagcact actacaaagt ggactactcc aggttccaca aaacttacga
     1321 agtccccaac actccccttt gtagtgccag agacttagca gaaaagaaat atatcctctc
     1381 aaatgcaaat tcattttgct atgaaaatga agttgccctc acaagcaaag aggaagacga
     1441 cagtgaaaat ggagttccag aaagcactag tacggacacg ccccctgaca tagaccttca
     1501 caaccaggca agtgtacctc tagagcccag gcccttacgg cgagagtcgg agatatgaac
     1561 ccagctttct tgtacaaagt ggtgataatc gaattccacg gggttggggt tgcgcctttt
     1621 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     1681 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     1741 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     1801 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     1861 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     1921 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     1981 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     2041 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     2101 ctctccccag ggggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
     2161 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2221 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2281 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     2341 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2401 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2461 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2521 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2581 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     2641 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2701 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2761 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2821 cactctcggc atggacgagc tgtacaaggc tagcggatca ggtcaatgta ctaactacgc
     2881 tttgttgaag cttgccggcg atgttgaaag taaccccggt cctggatcca tgtccagact
     2941 ggacaagagc aaagtcataa acggcgctct ggaattactc aatggagtcg gtatcgaagg
     3001 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca
     3061 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca
     3121 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa
     3181 gtcattccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
     3241 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
     3301 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
     3361 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
     3421 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
     3481 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
     3541 aagcggcggg ccggcctcga gcgaattcca gtacctgcca gatacagacg atcgtcaccg
     3601 gattgaggag aaacgtaaaa ggacatatga gaccttcaag agcatcatga agaagagtcc
     3661 tttcagcgga cccaccgacc cccggcctcc acctcgacgc attgctgtgc cttcccgcag
     3721 ctcagcttct gtccccaagc cagcacccca gccctatccc tttacgtcat ccctgagcac
     3781 catcaactat gatgagtttc ccaccatggt gtttccttct gggcagatca gccaggcctc
     3841 ggccttggcc ccggcccctc cccaagtcct gccccaggct ccagcccctg cccctgctcc
     3901 agccatggta tcagctctgg cccaggcccc agcccctgtc ccagtcctag ccccaggccc
     3961 tcctcaggct gtggccccac ctgcccccaa gcccacccag gctggggaag gaacgctgtc
     4021 agaggccctg ctgcagctgc agtttgatga tgaagacctg ggggccttgc ttggcaacag
     4081 cacagaccca gctgtgttca cagacctggc atccgtcgac aactccgagt ttcagcagct
     4141 gctgaaccag ggcatacctg tggcccccca cacaactgag cccatgctga tggagtaccc
     4201 tgaggctata actcgcctag tgacaggggc ccagaggccc cccgacccag ctcctgctcc
     4261 actgggggcc ccggggctcc ccaatggcct cctttcagga gatgaagact tctcctccat
     4321 tgcggacatg gacttctcag ccctgctgag tcagatcagc tcctaaagat ctgatcaagc
     4381 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4441 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4501 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4561 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4621 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4681 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4741 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4801 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4861 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4921 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     4981 gataccgtcg acctcgaggt tcgctttaag accaatgact tacaaggcag ctgtagatct
     5041 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5101 agatgggatc aattcaccat gggaataact tcgtatagca tacattatac gaagttatgc
     5161 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5221 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5281 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5341 tgtggaaaat ctctagcagc atctagttga atcgccagtg tgtcgagcta gcagatcttt
     5401 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     5461 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     5521 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     5581 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
     5641 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     5701 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     5761 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     5821 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca
     5881 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5941 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6001 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt
     6061 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6181 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6241 gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6301 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6361 caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga
     6421 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6481 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6541 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac ggatacctgt
     6781 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6841 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6901 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6961 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7021 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7081 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7141 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7201 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7261 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7321 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7381 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7441 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7501 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7561 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7621 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7681 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7741 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7801 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7861 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7921 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7981 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8041 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8101 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8161 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8221 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8281 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8341 ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat
     8401 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     8461 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     8521 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     8581 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     8641 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     8701 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggga
     8761 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     8821 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8881 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8941 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9001 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     9061 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     9121 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     9181 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     9241 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     9301 gattcgcagt taatactggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     9361 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     9421 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     9481 agatagagga agagcaaaac aaaagtaaga aaaaagcaca gcaagcagca ggatcttcag
     9541 acctggaaat tccctacaat ccccaaagtc aaggagtagt agaatctatg aataaagaat
     9601 taaagaaaat tataggacag gtaagagatc aggctgaaca tcttaagaca gcagtacaaa
     9661 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     9721 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     9781 caaaaattca aaattttcgg gtttattaca gggacagcag aaatccactt tggaaaggac
     9841 cagcaaagct cctctggaaa ggtgaagggg cagtagtaat acaagataat agtgacataa
     9901 aagtagtgcc aagaagaaaa gcaaagatca ttagggatta tggaaaacag atggcaggtg
     9961 atgattgtgt ggcaagtaga caggatgagg attagaacat ggaaaagttt agtaaaacac
    10021 cataaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
    10081 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
    10141 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
    10201 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
    10261 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
    10321 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
    10381 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
    10441 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    10501 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    10561 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    10621 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
    10681 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
    10741 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
    10801 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
    10861 acggatctcg acggtatcta gtgattatcg gatcaacttt gtatagaaaa gttgggtaca
    10921 actcccacta gacatatcct actctatcat tgatagagtg acccttccat agacatatcc
    10981 t
//
