LOCUS       sequence-218914-       10513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of Erythropoietin receptor without 
            cytoplasmic tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10513)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-EpoRtm
FEATURES             Location/Qualifiers
     source          1..10513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    146..164
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    226..250
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1072..1096)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1113..1623
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1542..1560
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1645..1647,1648..1650,1651..2361)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1645..1647,1648..1650,1651..2361)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1690..1711)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1951..1970)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2298..2319
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2377..2436
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             3505..3876
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    3902..4490
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3955..3975)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4373..4384)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4493..4509)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4494..4510)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(4638..4671)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4691..4871
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4909..4928)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4974..5029
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5028..5047)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5390..5406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5390..5406)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5403..5425)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5414..5430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5438..5444,5445..5462,5463..5468))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5483..5504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5563..5758
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5609..5744
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5764..5898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5801..5820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5855..5874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5966..5983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6137..6724)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6217..6236)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6895..7686,7687..7755))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7518..7537
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7756..7860)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7891..8270
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8272..8529
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8576..8701
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9193..9310
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9670..9903
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10088..10132
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    10407..10427
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    10454..10472
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    10495..10513
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 gacccttcca ctaggttcac ttttctctat cattgatagg gagtggtaaa caattgatgg
       61 acaaaatcta tcaatgatag agagtgcatg ttactagtat tgttcttctc tatcattgat
      121 agtgactttg cctaatcact tagcaactct atcattgata gagttctagt attagcaagg
      181 cctttcctct atataaggaa gttcatttca tttggagaag atcccccaag tttgtacaaa
      241 aaagcaggct tcaccatgga ccaactcagg gtggcccgct ggcctcgggt tagccccctg
      301 tgtctcctac ttgctggggc agcctgggca tcctcaccca gcctcccgga ccccaagttt
      361 gagagcaaag ccgccctgct ggcatcccgg ggctccgaag aacttctatg cttcacccag
      421 cgcttggaag acttggtgtg tttctgggag gaagcggcga actccgggat gggcttcaac
      481 tacagcttct cttaccagct cgaaggtgaa tcaagaaagt catgtcgcct gcaccaggcg
      541 cccaccgtcc gcggctccat gcgtttctgg tgttcactgc cgaccgcgga cacgtcgagt
      601 tttgtgccac tggagctgca ggtgaccgag gcctccggat ctcctcgcta ccaccgcatc
      661 atccatatca atgaagtagt gctcctggac gcccccgcgg ggctgctggc gcgccgagca
      721 gaagagggca gccacgtggt gctgcggtgg ctgccacctc ccggggctcc tatgaccacc
      781 cacatccgct acgaggtgga cgtgtcagca ggcaaccggg ctggggggac acaaagggtg
      841 gaggtcctgg aaggccgcac tgagtgtgtc ctgagcaacc tgcggggcgg gacgcgctac
      901 accttcgctg ttcgagcacg catggccgag ccgagcttca gcggattctg gagcgcctgg
      961 tctgagcccg cgtcgctact gactgctagc gacttggacc ctctcatctt gacgctgtct
     1021 ctcattctcg tcctcatctc actgttgctg actgtgctgg ccctgctgta gacccagctt
     1081 tcttgtacaa agtggtgata atcgaattcc acggggttgg ggttgcgcct tttccaaggc
     1141 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     1201 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     1261 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     1321 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     1381 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     1441 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     1501 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     1561 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     1621 cagggggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     1681 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1741 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1801 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     1861 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1921 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1981 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     2041 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2101 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     2161 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2221 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     2281 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2341 ggcatggacg agctgtacaa ggctagcgga tcaggtcaat gtactaacta cgctttgttg
     2401 aagcttgccg gcgatgttga aagtaacccc ggtcctggat ccatgtccag actggacaag
     2461 agcaaagtca taaacggcgc tctggaatta ctcaatggag tcggtatcga aggcctgacg
     2521 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
     2581 aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac
     2641 ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcattc
     2701 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
     2761 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
     2821 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
     2881 gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
     2941 cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc
     3001 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
     3061 gggccggcct cgagcgaatt ccagtacctg ccagatacag acgatcgtca ccggattgag
     3121 gagaaacgta aaaggacata tgagaccttc aagagcatca tgaagaagag tcctttcagc
     3181 ggacccaccg acccccggcc tccacctcga cgcattgctg tgccttcccg cagctcagct
     3241 tctgtcccca agccagcacc ccagccctat ccctttacgt catccctgag caccatcaac
     3301 tatgatgagt ttcccaccat ggtgtttcct tctgggcaga tcagccaggc ctcggccttg
     3361 gccccggccc ctccccaagt cctgccccag gctccagccc ctgcccctgc tccagccatg
     3421 gtatcagctc tggcccaggc cccagcccct gtcccagtcc tagccccagg ccctcctcag
     3481 gctgtggccc cacctgcccc caagcccacc caggctgggg aaggaacgct gtcagaggcc
     3541 ctgctgcagc tgcagtttga tgatgaagac ctgggggcct tgcttggcaa cagcacagac
     3601 ccagctgtgt tcacagacct ggcatccgtc gacaactccg agtttcagca gctgctgaac
     3661 cagggcatac ctgtggcccc ccacacaact gagcccatgc tgatggagta ccctgaggct
     3721 ataactcgcc tagtgacagg ggcccagagg ccccccgacc cagctcctgc tccactgggg
     3781 gccccggggc tccccaatgg cctcctttca ggagatgaag acttctcctc cattgcggac
     3841 atggacttct cagccctgct gagtcagatc agctcctaaa gatctgatca agcttatcga
     3901 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3961 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4021 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4081 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4141 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4201 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4261 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     4321 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     4381 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     4441 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     4501 tcgacctcga ggttcgcttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     4561 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatggg
     4621 atcaattcac catgggaata acttcgtata gcatacatta tacgaagtta tgctgctttt
     4681 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4741 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4801 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4861 aatctctagc agcatctagt tgaatcgcca gtgtgtcgag ctagcagatc tttttccctc
     4921 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     4981 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     5041 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     5101 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     5161 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     5221 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     5281 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     5341 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     5401 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5461 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5521 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
     5581 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5641 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5701 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5761 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5821 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5881 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5941 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6001 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6061 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6121 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6181 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6241 gaagctccct cgtgcgctct cctgttccga ccctgccgct tacggatacc tgtccgcctt
     6301 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6361 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6421 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6481 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6541 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6601 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6661 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6721 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6781 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6841 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6901 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6961 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7021 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7081 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7141 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7201 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7261 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7321 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7381 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7441 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7501 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7561 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7621 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7681 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7741 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7801 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7861 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
     7921 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     7981 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     8041 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     8101 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     8161 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     8221 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ggaggcgtgg
     8281 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     8341 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8401 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8461 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8521 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     8581 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     8641 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     8701 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     8761 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     8821 agttaatact ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     8881 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     8941 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     9001 ggaagagcaa aacaaaagta agaaaaaagc acagcaagca gcaggatctt cagacctgga
     9061 aattccctac aatccccaaa gtcaaggagt agtagaatct atgaataaag aattaaagaa
     9121 aattatagga caggtaagag atcaggctga acatcttaag acagcagtac aaatggcagt
     9181 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     9241 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     9301 tcaaaatttt cgggtttatt acagggacag cagaaatcca ctttggaaag gaccagcaaa
     9361 gctcctctgg aaaggtgaag gggcagtagt aatacaagat aatagtgaca taaaagtagt
     9421 gccaagaaga aaagcaaaga tcattaggga ttatggaaaa cagatggcag gtgatgattg
     9481 tgtggcaagt agacaggatg aggattagaa catggaaaag tttagtaaaa caccataagg
     9541 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     9601 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     9661 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     9721 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     9781 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     9841 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     9901 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9961 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    10021 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    10081 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    10141 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    10201 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    10261 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
    10321 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
    10381 tcgacggtat ctagtgatta tcggatcaac tttgtataga aaagttgggt acaactccca
    10441 ctagacatat cctactctat cattgataga gtgacccttc catagacata tcctactcta
    10501 tcattgatag agt
//
