LOCUS       sequence-218919-        9854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of Erythropoietin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9854)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCLXEBR-pTF-Epo
FEATURES             Location/Qualifiers
     source          1..9854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(610..634)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     671..687
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     672..688
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    703..820
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        877..1088
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             1107..1502
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1512..1571
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             2634..3005
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    3031..3619
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3084..3104)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3502..3513)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3761..3794)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             3814..3994
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4032..4051)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4097..4152
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4151..4170)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4513..4529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4513..4529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4526..4548)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4537..4553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4561..4567,4568..4585,4586..4591))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4606..4627
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4686..4881
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4732..4867
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4887..5021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4924..4943)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4978..4997
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5089..5106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5260..5848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5340..5359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6019..6810,6811..6879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6642..6661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6880..6984)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7015..7394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             7396..7653
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7700..7825
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8317..8434
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8794..9027
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9212..9256
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    9547..9565
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    9588..9606
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    9752..9770
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 acaagtttgt acaaaaaagc aggcttcacc atgggggtgc ccgaacgtcc caccctgctg
       61 cttttactat ccttgctact gattcctctg ggcctcccag tcctctgcgc tcccccacgc
      121 ctcatttgcg acagtcgcgt tctggagagg tacatcttgg aggccaagga ggcagaaaat
      181 gtcacaatgg gctgtgcaga aggtcccaga ctgagtgaga atattaccgt cccagatacc
      241 aaagtcaact tctacgcttg gaaaagaatg aaggtggaag aacaggctgt agaagtttgg
      301 caaggcctgt ctctgctctc agaagccatc ctgcaggccc aggctctgca ggccaattcc
      361 tcccagccac cagagagtct tcagcttcat atagacaaag ccatcagtgg gctacgtagc
      421 ctcacttcac tgcttcgggt gctgggagct cagaaggaat tgatgtcgcc tccagacgcc
      481 acccaagccg ctccactccg aacactcaca gcggatactt tctgcaagct cttccgggtc
      541 tactccaact tcctccgggg gaaactgaag ctgtacacgg gggaggcctg caggagaggg
      601 gacaggtgaa cccagctttc ttgtacaaag tggtgattcg acctgcaggc atgcaagctc
      661 gggccccccc tcgaggtcga cggtatcgat aagcttgata acttttaaaa gaaaaggggg
      721 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      781 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg agactagcct
      841 cgagaagctt gatcgatggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      901 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      961 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     1021 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     1081 gaacacaggt gtcgtgacgc ggatccatgg ccaagccttt gtctcaagaa gaatccaccc
     1141 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     1201 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     1261 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     1321 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     1381 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     1441 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     1501 gcggatcagg tcaatgtact aactacgctt tgttgaagct tgccggcgat gttgaaagta
     1561 accccggtcc tatgtccaga ctggacaaga gcaaagtcat aaacggcgct ctggaattac
     1621 tcaatggagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
     1681 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa
     1741 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
     1801 actttctgcg gaacaacgcc aagtcattcc gctgtgctct cctctcacat cgcgacgggg
     1861 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
     1921 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
     1981 tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg
     2041 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
     2101 tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
     2161 tggagaaaca gctaaagtgc gaaagcggcg ggccggcctc gagcgaattc cagtacctgc
     2221 cagatacaga cgatcgtcac cggattgagg agaaacgtaa aaggacatat gagaccttca
     2281 agagcatcat gaagaagagt cctttcagcg gacccaccga cccccggcct ccacctcgac
     2341 gcattgctgt gccttcccgc agctcagctt ctgtccccaa gccagcaccc cagccctatc
     2401 cctttacgtc atccctgagc accatcaact atgatgagtt tcccaccatg gtgtttcctt
     2461 ctgggcagat cagccaggcc tcggccttgg ccccggcccc tccccaagtc ctgccccagg
     2521 ctccagcccc tgcccctgct ccagccatgg tatcagctct ggcccaggcc ccagcccctg
     2581 tcccagtcct agccccaggc cctcctcagg ctgtggcccc acctgccccc aagcccaccc
     2641 aggctgggga aggaacgctg tcagaggccc tgctgcagct gcagtttgat gatgaagacc
     2701 tgggggcctt gcttggcaac agcacagacc cagctgtgtt cacagacctg gcatccgtcg
     2761 acaactccga gtttcagcag ctgctgaacc agggcatacc tgtggccccc cacacaactg
     2821 agcccatgct gatggagtac cctgaggcta taactcgcct agtgacaggg gcccagaggc
     2881 cccccgaccc agctcctgct ccactggggg ccccggggct ccccaatggc ctcctttcag
     2941 gagatgaaga cttctcctcc attgcggaca tggacttctc agccctgctg agtcagatca
     3001 gctcctaaag atccgcgtcg tacggtcgac aatcaacctc tggattacaa aatttgtgaa
     3061 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3121 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3181 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3241 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3301 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3361 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3421 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3481 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3541 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3601 ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca
     3661 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     3721 actcccaacg aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca
     3781 ttatacgaag ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     3841 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     3901 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3961 agaccctttt agtcagtgtg gaaaatctct agcagcatct agttgaatcg ccagtgtgtc
     4021 gagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     4081 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     4141 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     4201 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     4261 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     4321 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     4381 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     4441 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     4501 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4561 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4621 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     4681 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4741 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4801 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4861 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat
     4921 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4981 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg
     5041 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5101 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5161 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5221 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5281 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5341 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5401 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5461 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5521 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5581 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5641 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5701 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5761 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5821 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5881 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5941 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6001 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6061 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6121 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6181 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6241 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6301 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6361 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6421 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6481 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6541 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6601 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6661 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6721 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6781 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6841 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6901 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6961 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt
     7021 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     7081 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     7141 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     7201 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     7261 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     7321 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     7381 tcgctattac catgggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     7441 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     7501 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7561 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7621 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     7681 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     7741 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     7801 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     7861 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     7921 aagcagggag ctagaacgat tcgcagttaa tactggcctg ttagaaacat cagaaggctg
     7981 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     8041 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     8101 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagaaaa aagcacagca
     8161 agcagcagga tcttcagacc tggaaattcc ctacaatccc caaagtcaag gagtagtaga
     8221 atctatgaat aaagaattaa agaaaattat aggacaggta agagatcagg ctgaacatct
     8281 taagacagca gtacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     8341 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     8401 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaaa
     8461 tccactttgg aaaggaccag caaagctcct ctggaaaggt gaaggggcag tagtaataca
     8521 agataatagt gacataaaag tagtgccaag aagaaaagca aagatcatta gggattatgg
     8581 aaaacagatg gcaggtgatg attgtgtggc aagtagacag gatgaggatt agaacatgga
     8641 aaagtttagt aaaacaccat aaggaggaga tatgagggac aattggagaa gtgaattata
     8701 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     8761 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     8821 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     8881 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8941 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     9001 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     9061 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     9121 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     9181 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     9241 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     9301 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     9361 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     9421 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     9481 atccattcga ttagtgaacg gatctcgacg gtatcggttc ggtacaactc ccactagaca
     9541 tatcctactc tatcattgat agagtgaccc ttccatagac atatcctact ctatcattga
     9601 tagagtgacc cttccactag gttcactttt ctctatcatt gatagggagt ggtaaacaat
     9661 tgatggacaa aatctatcaa tgatagagag tgcatgttac tagtattgtt cttctctatc
     9721 attgatagtg actttgccta atcacttagc aactctatca ttgatagagt tctagtatta
     9781 gcaaggcctt tcctctatat aaggaagttc atttcatttg gagaagatcc ccggggatcc
     9841 tctagagtcg aatc
//
