LOCUS       sequence-218920-       10276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of Erythropoietin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10276)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 10276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-Epo
FEATURES             Location/Qualifiers
     source          1..10276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             386..643
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    690..815
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1424
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    1784..2017
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2202..2246
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2521..2541
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2568..2586
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2609..2627
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2773..2791
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    2853..2877
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3462..3486)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3503..4013
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3932..3950
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4029..4038
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4035..4037,4038..4040,4041..4751)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4035..4037,4038..4040,4041..4751)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4080..4101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4341..4360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4688..4709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4767..4826
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5895..6266
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    6292..6880
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6345..6365)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6763..6774)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6883..6899)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6884..6900)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(7028..7061)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7081..7261
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7299..7318)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7364..7419
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7418..7437)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7780..7796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7780..7796)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7793..7815)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7804..7820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7828..7834,7835..7852,7853..7858))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7873..7894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7953..8148
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7999..8134
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8154..8288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8191..8210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8245..8264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8356..8373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8527..9114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8607..8626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9285..10076,10077..10145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9908..9927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10146..10250)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catgggaggc gtggcctggg cgggactggg gagtggcgag
      421 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
      481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      661 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      721 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      781 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      841 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
      901 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tactggcctg ttagaaacat
      961 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1021 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1081 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagaaaa
     1141 aagcacagca agcagcagga tcttcagacc tggaaattcc ctacaatccc caaagtcaag
     1201 gagtagtaga atctatgaat aaagaattaa agaaaattat aggacaggta agagatcagg
     1261 ctgaacatct taagacagca gtacaaatgg cagtattcat ccacaatttt aaaagaaaag
     1321 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1381 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1441 acagcagaaa tccactttgg aaaggaccag caaagctcct ctggaaaggt gaaggggcag
     1501 tagtaataca agataatagt gacataaaag tagtgccaag aagaaaagca aagatcatta
     1561 gggattatgg aaaacagatg gcaggtgatg attgtgtggc aagtagacag gatgaggatt
     1621 agaacatgga aaagtttagt aaaacaccat aaggaggaga tatgagggac aattggagaa
     1681 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1741 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1801 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1861 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1921 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1981 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     2041 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     2101 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     2161 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     2221 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2281 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2341 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2401 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2461 acagagacag atccattcga ttagtgaacg gatctcgacg gtatctagtg attatcggat
     2521 caactttgta tagaaaagtt gggtacaact cccactagac atatcctact ctatcattga
     2581 tagagtgacc cttccataga catatcctac tctatcattg atagagtgac ccttccacta
     2641 ggttcacttt tctctatcat tgatagggag tggtaaacaa ttgatggaca aaatctatca
     2701 atgatagaga gtgcatgtta ctagtattgt tcttctctat cattgatagt gactttgcct
     2761 aatcacttag caactctatc attgatagag ttctagtatt agcaaggcct ttcctctata
     2821 taaggaagtt catttcattt ggagaagatc ccccaagttt gtacaaaaaa gcaggcttca
     2881 ccatgggggt gcccgaacgt cccaccctgc tgcttttact atccttgcta ctgattcctc
     2941 tgggcctccc agtcctctgc gctcccccac gcctcatttg cgacagtcgc gttctggaga
     3001 ggtacatctt ggaggccaag gaggcagaaa atgtcacaat gggctgtgca gaaggtccca
     3061 gactgagtga gaatattacc gtcccagata ccaaagtcaa cttctacgct tggaaaagaa
     3121 tgaaggtgga agaacaggct gtagaagttt ggcaaggcct gtctctgctc tcagaagcca
     3181 tcctgcaggc ccaggctctg caggccaatt cctcccagcc accagagagt cttcagcttc
     3241 atatagacaa agccatcagt gggctacgta gcctcacttc actgcttcgg gtgctgggag
     3301 ctcagaagga attgatgtcg cctccagacg ccacccaagc cgctccactc cgaacactca
     3361 cagcggatac tttctgcaag ctcttccggg tctactccaa cttcctccgg gggaaactga
     3421 agctgtacac gggggaggcc tgcaggagag gggacaggtg aacccagctt tcttgtacaa
     3481 agtggtgata atcgaattcc acggggttgg ggttgcgcct tttccaaggc agccctgggt
     3541 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     3601 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     3661 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     3721 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     3781 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     3841 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     3901 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     3961 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
     4021 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     4081 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     4141 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     4201 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     4261 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4321 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     4381 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     4441 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     4501 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     4561 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4621 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     4681 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     4741 agctgtacaa ggctagcgga tcaggtcaat gtactaacta cgctttgttg aagcttgccg
     4801 gcgatgttga aagtaacccc ggtcctggat ccatgtccag actggacaag agcaaagtca
     4861 taaacggcgc tctggaatta ctcaatggag tcggtatcga aggcctgacg acaaggaaac
     4921 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
     4981 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc
     5041 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcattc cgctgtgctc
     5101 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
     5161 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
     5221 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
     5281 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
     5341 tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
     5401 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccggcct
     5461 cgagcgaatt ccagtacctg ccagatacag acgatcgtca ccggattgag gagaaacgta
     5521 aaaggacata tgagaccttc aagagcatca tgaagaagag tcctttcagc ggacccaccg
     5581 acccccggcc tccacctcga cgcattgctg tgccttcccg cagctcagct tctgtcccca
     5641 agccagcacc ccagccctat ccctttacgt catccctgag caccatcaac tatgatgagt
     5701 ttcccaccat ggtgtttcct tctgggcaga tcagccaggc ctcggccttg gccccggccc
     5761 ctccccaagt cctgccccag gctccagccc ctgcccctgc tccagccatg gtatcagctc
     5821 tggcccaggc cccagcccct gtcccagtcc tagccccagg ccctcctcag gctgtggccc
     5881 cacctgcccc caagcccacc caggctgggg aaggaacgct gtcagaggcc ctgctgcagc
     5941 tgcagtttga tgatgaagac ctgggggcct tgcttggcaa cagcacagac ccagctgtgt
     6001 tcacagacct ggcatccgtc gacaactccg agtttcagca gctgctgaac cagggcatac
     6061 ctgtggcccc ccacacaact gagcccatgc tgatggagta ccctgaggct ataactcgcc
     6121 tagtgacagg ggcccagagg ccccccgacc cagctcctgc tccactgggg gccccggggc
     6181 tccccaatgg cctcctttca ggagatgaag acttctcctc cattgcggac atggacttct
     6241 cagccctgct gagtcagatc agctcctaaa gatctgatca agcttatcga taatcaacct
     6301 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6361 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6421 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6481 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6541 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6601 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6661 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6721 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6781 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6841 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     6901 ggttcgcttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6961 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatggg atcaattcac
     7021 catgggaata acttcgtata gcatacatta tacgaagtta tgctgctttt tgcttgtact
     7081 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7141 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7201 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7261 agcatctagt tgaatcgcca gtgtgtcgag ctagcagatc tttttccctc tgccaaaaat
     7321 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     7381 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     7441 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     7501 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     7561 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     7621 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     7681 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     7741 agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7801 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7861 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7921 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
     7981 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8041 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8101 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
     8161 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8221 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8281 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8341 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8401 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8461 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8521 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8581 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8641 cgtgcgctct cctgttccga ccctgccgct tacggatacc tgtccgcctt tctcccttcg
     8701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8941 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9061 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9181 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9241 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9301 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9361 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9421 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9481 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9541 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9601 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9661 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9721 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9781 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9841 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9901 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9961 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10021 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10081 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10141 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10201 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10261 cgaaaagtgc cacctg
//
