LOCUS       sequence-218922-        9840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the V12-mutant form of the ki-Ras 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9840)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCLXEBR-pTF-kRasV12
FEATURES             Location/Qualifiers
     source          1..9840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    85..109
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(112..144,145..147,148..678)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(680..704)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     741..757
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     742..758
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    773..890
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        947..1158
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             1177..1572
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1582..1641
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             2704..3075
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    3101..3689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3154..3174)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3572..3583)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3831..3864)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             3884..4064
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4102..4121)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4167..4222
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4221..4240)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4583..4599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4583..4599)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4596..4618)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4607..4623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4631..4637,4638..4655,4656..4661))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4676..4697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4756..4951
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4802..4937
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4957..5091
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4994..5013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5048..5067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5159..5176)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5330..5918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5410..5429)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6089..6880,6881..6949))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6712..6731
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6950..7054)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7085..7464
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             7466..7723
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7770..7895
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8387..8504
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8864..9097
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9282..9326
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    9617..9635
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    9658..9676
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    9822..9840
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 tctagtatta gcaaggcctt tcctctatat aaggaagttc atttcatttg gagaagatcc
       61 ccggggatcc tctagagtcg aatcacaagt ttgtacaaaa aagttggcac catgactgaa
      121 tataaacttg tggtagttgg agctgttggc gtaggcaaga gtgccttgac gatacagcta
      181 attcagaatc attttgtgga cgaatatgat ccaacaatag aggattccta caggaagcaa
      241 gtagtaattg atggagaaac ctgtctcttg gatattctcg acacagcagg tcaagaggag
      301 tacagtgcaa tgagggacca gtacatgagg actggggagg gctttctttg tgtatttgcc
      361 ataaataata ctaaatcatt tgaagatatt caccattata gagaacaaat taaaagagtt
      421 aaggactctg aagatgtacc tatggtccta gtaggaaata aatgtgattt gccttccaga
      481 acagtagaca caaaacaggc tcaggactta gcaagaagtt atggaattcc ttttattgaa
      541 acatcagcaa agacaagaca gggtgttgat gatgccttct atacattagt tcgagaaatt
      601 cgaaaacata aagaaaagat gagcaaagat ggtaaaaaga agaaaaagaa gtcaaagaca
      661 aagtgtgtaa ttatgtaatt gccaactttc ttgtacaaag tggtgattcg acctgcaggc
      721 atgcaagctc gggccccccc tcgaggtcga cggtatcgat aagcttgata acttttaaaa
      781 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      841 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg
      901 agactagcct cgagaagctt gatcgatggc tccggtgccc gtcagtgggc agagcgcaca
      961 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     1021 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     1081 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     1141 tttgccgcca gaacacaggt gtcgtgacgc ggatccatgg ccaagccttt gtctcaagaa
     1201 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
     1261 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
     1321 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
     1381 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
     1441 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
     1501 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
     1561 gtgtgggagg gcggatcagg tcaatgtact aactacgctt tgttgaagct tgccggcgat
     1621 gttgaaagta accccggtcc tatgtccaga ctggacaaga gcaaagtcat aaacggcgct
     1681 ctggaattac tcaatggagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     1741 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     1801 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
     1861 tcatggcaag actttctgcg gaacaacgcc aagtcattcc gctgtgctct cctctcacat
     1921 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     1981 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     2041 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     2101 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca
     2161 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     2221 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccggcctc gagcgaattc
     2281 cagtacctgc cagatacaga cgatcgtcac cggattgagg agaaacgtaa aaggacatat
     2341 gagaccttca agagcatcat gaagaagagt cctttcagcg gacccaccga cccccggcct
     2401 ccacctcgac gcattgctgt gccttcccgc agctcagctt ctgtccccaa gccagcaccc
     2461 cagccctatc cctttacgtc atccctgagc accatcaact atgatgagtt tcccaccatg
     2521 gtgtttcctt ctgggcagat cagccaggcc tcggccttgg ccccggcccc tccccaagtc
     2581 ctgccccagg ctccagcccc tgcccctgct ccagccatgg tatcagctct ggcccaggcc
     2641 ccagcccctg tcccagtcct agccccaggc cctcctcagg ctgtggcccc acctgccccc
     2701 aagcccaccc aggctgggga aggaacgctg tcagaggccc tgctgcagct gcagtttgat
     2761 gatgaagacc tgggggcctt gcttggcaac agcacagacc cagctgtgtt cacagacctg
     2821 gcatccgtcg acaactccga gtttcagcag ctgctgaacc agggcatacc tgtggccccc
     2881 cacacaactg agcccatgct gatggagtac cctgaggcta taactcgcct agtgacaggg
     2941 gcccagaggc cccccgaccc agctcctgct ccactggggg ccccggggct ccccaatggc
     3001 ctcctttcag gagatgaaga cttctcctcc attgcggaca tggacttctc agccctgctg
     3061 agtcagatca gctcctaaag atccgcgtcg tacggtcgac aatcaacctc tggattacaa
     3121 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3181 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3241 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3301 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3361 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3421 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3481 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     3541 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3601 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3661 tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca
     3721 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     3781 gggctaattc actcccaacg aagacaagat gggatcaatt caccatggga ataacttcgt
     3841 atagcataca ttatacgaag ttatgctgct ttttgcttgt actgggtctc tctggttaga
     3901 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3961 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4021 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agttgaatcg
     4081 ccagtgtgtc gagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     4141 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     4201 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     4261 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     4321 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     4381 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     4441 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     4501 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     4561 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4621 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4681 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     4741 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4801 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4861 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4921 aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat
     4981 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5041 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt
     5101 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5161 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5221 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5281 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5341 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5401 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5461 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5521 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5581 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5641 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5701 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5761 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5821 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5881 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5941 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6001 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6061 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6121 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6181 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6241 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6301 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6361 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6421 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6481 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6541 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6601 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6661 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6721 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6781 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6841 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6901 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6961 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7021 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7081 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     7141 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     7201 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     7261 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     7321 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     7381 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     7441 gtattagtca tcgctattac catgggaggc gtggcctggg cgggactggg gagtggcgag
     7501 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     7561 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7621 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7681 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     7741 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     7801 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     7861 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     7921 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     7981 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tactggcctg ttagaaacat
     8041 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8101 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     8161 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagaaaa
     8221 aagcacagca agcagcagga tcttcagacc tggaaattcc ctacaatccc caaagtcaag
     8281 gagtagtaga atctatgaat aaagaattaa agaaaattat aggacaggta agagatcagg
     8341 ctgaacatct taagacagca gtacaaatgg cagtattcat ccacaatttt aaaagaaaag
     8401 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     8461 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     8521 acagcagaaa tccactttgg aaaggaccag caaagctcct ctggaaaggt gaaggggcag
     8581 tagtaataca agataatagt gacataaaag tagtgccaag aagaaaagca aagatcatta
     8641 gggattatgg aaaacagatg gcaggtgatg attgtgtggc aagtagacag gatgaggatt
     8701 agaacatgga aaagtttagt aaaacaccat aaggaggaga tatgagggac aattggagaa
     8761 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     8821 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     8881 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     8941 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     9001 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     9061 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     9121 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     9181 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     9241 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     9301 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     9361 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     9421 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     9481 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     9541 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggttc ggtacaactc
     9601 ccactagaca tatcctactc tatcattgat agagtgaccc ttccatagac atatcctact
     9661 ctatcattga tagagtgacc cttccactag gttcactttt ctctatcatt gatagggagt
     9721 ggtaaacaat tgatggacaa aatctatcaa tgatagagag tgcatgttac tagtattgtt
     9781 cttctctatc attgatagtg actttgccta atcacttagc aactctatca ttgatagagt
//
