LOCUS       sequence-218928-       10467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the mBmi-1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Salmon P
  TITLE     Generation of human cell lines using lentiviral-mediated genetic 
            engineering.
  JOURNAL   Methods Mol Biol. 2013;945:417-48. doi: 
            10.1007/978-1-62703-125-7_25.
  PUBMED    23097121
REFERENCE   2  (bases 1 to 10467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2013"; volume: "945:417-48. doi"; pages: " 
            10.1007/978-1-62703-125-7_25"
COMMENT     Sequence Label: pCLXEBR-pTF-mBmi1
FEATURES             Location/Qualifiers
     source          1..10467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1198..1222)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1259..1275
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1260..1276
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1291..1408
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1465..1676
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             1695..2090
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2100..2159
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             3222..3593
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    3619..4207
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3672..3692)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4090..4101)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4349..4382)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4402..4582
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4620..4639)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4685..4740
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4739..4758)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5101..5117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5101..5117)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5114..5136)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5125..5141
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5149..5155,5156..5173,5174..5179))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5194..5215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5274..5469
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5320..5455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5475..5609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5512..5531)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5566..5585
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5677..5694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5848..6436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5928..5947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6607..7398,7399..7467))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7230..7249
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7468..7572)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7603..7982
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             7984..8241
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8288..8413
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8905..9022
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9382..9615
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9800..9844
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    10135..10153
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    10176..10194
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    10340..10358
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    10443..10467
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagtgagg aaccaattca gtcgactgga tccggtaccg aattaattcc ggctcggagt
       61 cttttatcaa gcagaaatgc atcgaacaac cagaatcaag atcactgagc taaatcccca
      121 cttaatgtgt gtcctgtgtg gagggtactt cattgatgcc actaccataa tagaatgtct
      181 acattccttc tgtaaaacat gtattgtacg ttacttggag accagcaagt attgtcctat
      241 ttgtgatgtc caggttcaca aaaccagacc actcctgaac ataaggtcag ataaaactct
      301 tcaagatatt gtatacaaat tagtcccagg gcttttcaaa aatgagatga agagaagaag
      361 agatttttat gcagctcacc cgtcagctga tgctgccaat ggctccaatg aagaccgagg
      421 agaagttgca gatgaggaga agaggattat aactgatgat gagataataa gcttgtctat
      481 tgagttcttt gatcagagca gattggatcg gaaagtaaat aaagagaagc ctaaggaaga
      541 ggtgaatgat aaaaggtact tacgatgccc agcagcaatg actgtgatgc acttgagaaa
      601 gtttctcagg agtaaaatgg acatacccaa tactttccag attgacgtca tgtatgaaga
      661 ggaaccttta aaggattact acacgctaat ggacattgcc tacatttata cctggagaag
      721 aaatggccca ctacctttga aatacagagt tcggccaact tgcaaaagaa tgaagatgag
      781 tcaccagagg gatggactga cgaatgctgg agagctggaa agtgactctg ggagtgacaa
      841 ggccaacagc ccagccggag gtgttccctc cacctcttcc tgtttgccca gtcccagcac
      901 tccagtgcag tctcctcacc cacagttccc tcacatttcc agtaccatga atggaaccag
      961 caacagcccc agtgctaacc accaatcttc ctttgccagt agacctcgaa aatcatcact
     1021 aaatgggtca tcagcaactt catctggtta ggactgttaa ggaaaagatt tttcaacccc
     1081 ctgatttagt taccttcatt cattacagct ttatagatgc ttaatacatg tgactgtcgt
     1141 ccagtttgct tccttttgta gtgactttaa atttggctgc tcgagggaat tatctagacc
     1201 cagctttctt gtacaaagtg gtgattcgac ctgcaggcat gcaagctcgg gccccccctc
     1261 gaggtcgacg gtatcgataa gcttgataac ttttaaaaga aaagggggga ttggggggta
     1321 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     1381 aaaacaaatt acaaaaattc aaaattttat cgatcacgag actagcctcg agaagcttga
     1441 tcgatggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     1501 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
     1561 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     1621 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtgt
     1681 cgtgacgcgg atccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     1741 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     1801 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     1861 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     1921 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     1981 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     2041 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ggatcaggtc
     2101 aatgtactaa ctacgctttg ttgaagcttg ccggcgatgt tgaaagtaac cccggtccta
     2161 tgtccagact ggacaagagc aaagtcataa acggcgctct ggaattactc aatggagtcg
     2221 gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc
     2281 tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg
     2341 acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga
     2401 acaacgccaa gtcattccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc
     2461 tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt
     2521 gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta
     2581 cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac
     2641 ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg
     2701 gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc
     2761 taaagtgcga aagcggcggg ccggcctcga gcgaattcca gtacctgcca gatacagacg
     2821 atcgtcaccg gattgaggag aaacgtaaaa ggacatatga gaccttcaag agcatcatga
     2881 agaagagtcc tttcagcgga cccaccgacc cccggcctcc acctcgacgc attgctgtgc
     2941 cttcccgcag ctcagcttct gtccccaagc cagcacccca gccctatccc tttacgtcat
     3001 ccctgagcac catcaactat gatgagtttc ccaccatggt gtttccttct gggcagatca
     3061 gccaggcctc ggccttggcc ccggcccctc cccaagtcct gccccaggct ccagcccctg
     3121 cccctgctcc agccatggta tcagctctgg cccaggcccc agcccctgtc ccagtcctag
     3181 ccccaggccc tcctcaggct gtggccccac ctgcccccaa gcccacccag gctggggaag
     3241 gaacgctgtc agaggccctg ctgcagctgc agtttgatga tgaagacctg ggggccttgc
     3301 ttggcaacag cacagaccca gctgtgttca cagacctggc atccgtcgac aactccgagt
     3361 ttcagcagct gctgaaccag ggcatacctg tggcccccca cacaactgag cccatgctga
     3421 tggagtaccc tgaggctata actcgcctag tgacaggggc ccagaggccc cccgacccag
     3481 ctcctgctcc actgggggcc ccggggctcc ccaatggcct cctttcagga gatgaagact
     3541 tctcctccat tgcggacatg gacttctcag ccctgctgag tcagatcagc tcctaaagat
     3601 ccgcgtcgta cggtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3661 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3721 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3781 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3841 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3901 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3961 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     4021 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     4081 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     4141 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     4201 tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     4261 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     4321 gacaagatgg gatcaattca ccatgggaat aacttcgtat agcatacatt atacgaagtt
     4381 atgctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4441 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4501 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4561 tcagtgtgga aaatctctag cagcatctag ttgaatcgcc agtgtgtcga gctagcagat
     4621 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     4681 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     4741 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     4801 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     4861 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     4921 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     4981 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     5041 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg
     5101 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5161 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5221 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc
     5281 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5341 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5401 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5461 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5521 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5581 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
     5641 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5701 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5761 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5821 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5881 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5941 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6001 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6061 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6121 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6181 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6241 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6301 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6361 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6421 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6481 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6541 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6601 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6661 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6721 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6781 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6841 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6901 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6961 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7021 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7081 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7141 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7201 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7261 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7321 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7381 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7441 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7501 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7561 aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga ttattgacta
     7621 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     7681 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     7741 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     7801 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     7861 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     7921 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     7981 tgggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     8041 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8101 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8161 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8221 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     8281 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     8341 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     8401 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     8461 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     8521 agaacgattc gcagttaata ctggcctgtt agaaacatca gaaggctgta gacaaatact
     8581 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8641 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     8701 agacaagata gaggaagagc aaaacaaaag taagaaaaaa gcacagcaag cagcaggatc
     8761 ttcagacctg gaaattccct acaatcccca aagtcaagga gtagtagaat ctatgaataa
     8821 agaattaaag aaaattatag gacaggtaag agatcaggct gaacatctta agacagcagt
     8881 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     8941 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     9001 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagaaatc cactttggaa
     9061 aggaccagca aagctcctct ggaaaggtga aggggcagta gtaatacaag ataatagtga
     9121 cataaaagta gtgccaagaa gaaaagcaaa gatcattagg gattatggaa aacagatggc
     9181 aggtgatgat tgtgtggcaa gtagacagga tgaggattag aacatggaaa agtttagtaa
     9241 aacaccataa ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     9301 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     9361 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     9421 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     9481 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     9541 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     9601 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     9661 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     9721 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9781 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9841 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9901 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9961 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
    10021 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
    10081 agtgaacgga tctcgacggt atcggttcgg tacaactccc actagacata tcctactcta
    10141 tcattgatag agtgaccctt ccatagacat atcctactct atcattgata gagtgaccct
    10201 tccactaggt tcacttttct ctatcattga tagggagtgg taaacaattg atggacaaaa
    10261 tctatcaatg atagagagtg catgttacta gtattgttct tctctatcat tgatagtgac
    10321 tttgcctaat cacttagcaa ctctatcatt gatagagttc tagtattagc aaggcctttc
    10381 ctctatataa ggaagttcat ttcatttgga gaagatcccc ggggatcctc tagagtcgaa
    10441 tcacaagttt gtacaaaaaa gcaggct
//
