LOCUS       sequence-218938-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ER-stress sensor - XBP1 splicing fluorescent reporter with 
            mNeonGreen (retroviral vector backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Nougarede A, Tesniere C, Ylanko J, Rimokh R, Gillet G, Andrews DW
  TITLE     Improved IRE1 and PERK Pathway Sensors for Multiplex Endoplasmic 
            Reticulum Stress Assay Reveal Stress Response to Nuclear Dyes Used 
            for Image Segmentation.
  JOURNAL   Assay Drug Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862.
  PUBMED    30088945
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Assay Drug 
            Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862."
COMMENT     Sequence Label: pLHCX-XBP1 mNeonGreen NLS
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..45
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             19..45
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             306..1010
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      558..567
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1032..1058
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
ORIGIN
        1 aagcttgaat tcaccatgta cccctacgac gtgcccgact acgccggcct tgtagttgag
       61 aaccaggagt taagacagcg cttggggatg gatgccctgg ttgctgaaga ggaggcggaa
      121 gccaagggga atgaagtgag gccagtggcc gggtctgctg agtccgcagc actcagacta
      181 cgtgcacctc tgcagcaggt gcaggcccag ttgtcacccc tccagaacat ctccccttgg
      241 attctggcgg tattgactct tcagattcag agtctgatat cctgttgggc attctaatag
      301 gatccgtgag caagggcgag gaggataaca tggcctctct cccagcgaca catgagttac
      361 acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc accggcaatc
      421 caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc cagttctccc
      481 cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc taccctgacg
      541 ggatgtcgcc tttccaggcc gccatggtag atggctccgg ataccaagtc catcgcacaa
      601 tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac gagggaagcc
      661 acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt cctgtgatga
      721 ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa gacttacccc aacgacaaaa
      781 ccatcatcag tacctttaag tggagttaca ccactggaaa tggcaagcgc taccggagca
      841 ctgcgcggac cacctacacc tttgccaagc caatggcggc taactatctg aagaaccagc
      901 cgatgtacgt gttccgtaag acggagctca agcactccaa gaccgagctc aacttcaagg
      961 agtggcaaaa ggcctttacc gatgtgatgg gcatggacga gctgtacaag gtcgacggtg
     1021 gatcaggggg acccgccgcc aagcgcgtga agctggacta atctagaatc gat
//
