LOCUS       sequence-218942-        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter 
            with mScarlet-I (retroviral vector backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Nougarede A, Tesniere C, Ylanko J, Rimokh R, Gillet G, Andrews DW
  TITLE     Improved IRE1 and PERK Pathway Sensors for Multiplex Endoplasmic 
            Reticulum Stress Assay Reveal Stress Response to Nuclear Dyes Used 
            for Image Segmentation.
  JOURNAL   Assay Drug Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862.
  PUBMED    30088945
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Assay Drug 
            Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862."
COMMENT     Sequence Label: pLHCX-ATF4 mScarlet NLS
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             375..401
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             375..401
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1137..1163
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
ORIGIN
        1 aagcttgaat tctctacttt gcccgcccac agatgtagtt ttctctgcgc gtgtgcgttt
       61 tccctcctcc ccgccctcag ggtccacggc caccatggcg tattaggggc agcagtgcct
      121 gcggcagcat tggcctttgc agcggcggca gcagcaccag gctctgcagc ggcaaccccc
      181 agcggcttaa gccatggcgc ttctcacggc attcagcagc agcgttgctg taaccgacaa
      241 agacaccttc gaattaagca cattcctcga ttccagcaaa gcaccgcaac atgaccgaaa
      301 tgagcttcct gagcagcgag gtgttggtgg gggacttgat gtcccccttc gaccagtcgg
      361 gtttgggggc tgaatacccc tacgacgtgc ccgactacgc cggtggatca gggggaggat
      421 ccgtctccaa aggggaagcg gtcattaaag agttcatgag gttcaaagtt catatggagg
      481 gcagtatgaa cggccatgaa tttgaaatcg agggcgaggg ggagggacgc ccttacgaag
      541 ggacacaaac agccaaactg aaggttacga aaggtgggcc acttcccttt agttgggata
      601 tactgtcacc tcaatttatg tacggcagcc gagccttcat taaacatccc gctgacattc
      661 cggattacta taagcagtcc tttccagagg ggtttaaatg ggagcgggtt atgaatttcg
      721 aggatggcgg ggccgtaact gtcacgcagg ataccagtct ggaagatggt accctgattt
      781 ataaggttaa gctcagagga acgaacttcc cgccagatgg tcccgtaatg caaaaaaaga
      841 cgatgggttg ggaggcatca actgaaagat tgtatcctga ggatggtgtc cttaagggtg
      901 atattaaaat ggctctgagg ctgaaggatg gcggaaggta tttggcagat ttcaaaacta
      961 catataaggc caagaagcct gttcagatgc caggtgccta taatgtggac agaaaacttg
     1021 acattacatc acacaacgag gattataccg tcgtggagca atatgagaga agtgagggcc
     1081 gacatagtac cggcggcatg gatgaactct ataaggtcga cggtggatca gggggacccg
     1141 ccgccaagcg cgtgaagctg gactaatcta gaatcgat
//
