LOCUS       sequence-218944-        1166 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter 
            with mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Nougarede A, Tesniere C, Ylanko J, Rimokh R, Gillet G, Andrews DW
  TITLE     Improved IRE1 and PERK Pathway Sensors for Multiplex Endoplasmic 
            Reticulum Stress Assay Reveal Stress Response to Nuclear Dyes Used 
            for Image Segmentation.
  JOURNAL   Assay Drug Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862.
  PUBMED    30088945
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Assay Drug 
            Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862."
COMMENT     Sequence Label: pLVX-ATF4 mScarlet NLS
FEATURES             Location/Qualifiers
     source          1..1166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             369..395
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             369..395
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1131..1157
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
ORIGIN
        1 gaattctcta ctttgcccgc ccacagatgt agttttctct gcgcgtgtgc gttttccctc
       61 ctccccgccc tcagggtcca cggccaccat ggcgtattag gggcagcagt gcctgcggca
      121 gcattggcct ttgcagcggc ggcagcagca ccaggctctg cagcggcaac ccccagcggc
      181 ttaagccatg gcgcttctca cggcattcag cagcagcgtt gctgtaaccg acaaagacac
      241 cttcgaatta agcacattcc tcgattccag caaagcaccg caacatgacc gaaatgagct
      301 tcctgagcag cgaggtgttg gtgggggact tgatgtcccc cttcgaccag tcgggtttgg
      361 gggctgaata cccctacgac gtgcccgact acgccggtgg atcaggggga ggatccgtct
      421 ccaaagggga agcggtcatt aaagagttca tgaggttcaa agttcatatg gagggcagta
      481 tgaacggcca tgaatttgaa atcgagggcg agggggaggg acgcccttac gaagggacac
      541 aaacagccaa actgaaggtt acgaaaggtg ggccacttcc ctttagttgg gatatactgt
      601 cacctcaatt tatgtacggc agccgagcct tcattaaaca tcccgctgac attccggatt
      661 actataagca gtcctttcca gaggggttta aatgggagcg ggttatgaat ttcgaggatg
      721 gcggggccgt aactgtcacg caggatacca gtctggaaga tggtaccctg atttataagg
      781 ttaagctcag aggaacgaac ttcccgccag atggtcccgt aatgcaaaaa aagacgatgg
      841 gttgggaggc atcaactgaa agattgtatc ctgaggatgg tgtccttaag ggtgatatta
      901 aaatggctct gaggctgaag gatggcggaa ggtatttggc agatttcaaa actacatata
      961 aggccaagaa gcctgttcag atgccaggtg cctataatgt ggacagaaaa cttgacatta
     1021 catcacacaa cgaggattat accgtcgtgg agcaatatga gagaagtgag ggccgacata
     1081 gtaccggcgg catggatgaa ctctataagg tcgacggtgg atcaggggga cccgccgcca
     1141 agcgcgtgaa gctggactaa tctaga
//
