LOCUS       sequence-218945-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ER-stress sensor - XBP1 splicing fluorescent reporter with 
            mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Nougarede A, Tesniere C, Ylanko J, Rimokh R, Gillet G, Andrews DW
  TITLE     Improved IRE1 and PERK Pathway Sensors for Multiplex Endoplasmic 
            Reticulum Stress Assay Reveal Stress Response to Nuclear Dyes Used 
            for Image Segmentation.
  JOURNAL   Assay Drug Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862.
  PUBMED    30088945
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Assay Drug 
            Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862."
COMMENT     Sequence Label: pLVX-XBP1 mNeonGreen NLS
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..39
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             13..39
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             300..1004
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      552..561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1026..1052
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
ORIGIN
        1 gaattcacca tgtaccccta cgacgtgccc gactacgccg gccttgtagt tgagaaccag
       61 gagttaagac agcgcttggg gatggatgcc ctggttgctg aagaggaggc ggaagccaag
      121 gggaatgaag tgaggccagt ggccgggtct gctgagtccg cagcactcag actacgtgca
      181 cctctgcagc aggtgcaggc ccagttgtca cccctccaga acatctcccc ttggattctg
      241 gcggtattga ctcttcagat tcagagtctg atatcctgtt gggcattcta ataggatccg
      301 tgagcaaggg cgaggaggat aacatggcct ctctcccagc gacacatgag ttacacatct
      361 ttggctccat caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg
      421 atggttatga ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga
      481 ttctggtccc tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt
      541 cgcctttcca ggccgccatg gtagatggct ccggatacca agtccatcgc acaatgcagt
      601 ttgaagatgg tgcctccctt actgttaact accgctacac ctacgaggga agccacatca
      661 aaggagaggc ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact
      721 cgctgaccgc tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca
      781 tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg agcactgcgc
      841 ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt
      901 acgtgttccg taagacggag ctcaagcact ccaagaccga gctcaacttc aaggagtggc
      961 aaaaggcctt taccgatgtg atgggcatgg acgagctgta caaggtcgac ggtggatcag
     1021 ggggacccgc cgccaagcgc gtgaagctgg actaatctag a
//
