LOCUS       sequence-218952-        9918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the Neugenin 3 transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P
  TITLE     Polyswitch lentivectors: ""all-in-one"" lentiviral vectors for 
            drug-inducible gene expression, live selection, and recombination 
            cloning.
  JOURNAL   Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27.
  PUBMED    21761975
REFERENCE   2  (bases 1 to 9918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2011-10"; volume: "22(10)"; pages: "1255-67. Epub 
            2011 Sep 27"
COMMENT     Sequence Label: pCLXEBR-pTF-Ngn3
FEATURES             Location/Qualifiers
     source          1..9918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(777..801)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     838..854
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     839..855
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    870..987
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1044..1255
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             1274..1669
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1679..1738
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             2801..3172
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    3198..3786
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3251..3271)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3669..3680)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3928..3961)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             3981..4161
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4199..4218)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4264..4319
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4318..4337)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4680..4696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4680..4696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4693..4715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4704..4720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4728..4734,4735..4752,4753..4758))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4773..4794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4853..5048
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4899..5034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5054..5188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5091..5110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5145..5164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5256..5273)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5427..6015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5507..5526)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6186..6977,6978..7046))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6809..6828
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7047..7151)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7182..7561
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             7563..7820
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7867..7992
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8484..8601
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8961..9194
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9379..9423
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    9714..9732
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    9755..9773
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 actctatcat tgatagagtt ctagtattag caaggccttt cctctatata aggaagttca
       61 tttcatttgg agaagatccc cggggatcct ctagagtcga atcacaagtt tgtacaaaaa
      121 agcaggcacc atgacgcctc aaccctcggg tgcgcccact gtccaagtga cccgtgagac
      181 ggagcggtcc ttccccagag cctcggaaga cgaagtgacc tgccccacgt ccgccccgcc
      241 cagccccact cgcacacggg ggaactgcgc agaggcggaa gagggaggct gccgaggggc
      301 cccgaggaag ctccgggcac ggcgcggggg acgcagccgg cctaagagcg agttggcact
      361 gagcaagcag cgacggagtc ggcgaaagaa ggccaacgac cgcgagcgca atcgaatgca
      421 caacctcaac tcggcactgg acgccctgcg cggtgtcctg cccaccttcc cagacgacgc
      481 gaagctcacc aagatcgaga cgctgcgctt cgcccacaac tacatctggg cgctgactca
      541 aacgctgcgc atagcggacc acagcttgta cgcgctggag ccgccggcgc cgcactgcgg
      601 ggagctgggc agcccaggcg gttcccccgg ggactggggg tccctctact ccccagtctc
      661 ccaggctggc agcctgagtc ccgccgcgtc gctggaggag cgacccgggc tgctgggggc
      721 cacctcttcc gcctgcttga gcccaggcag tctggctttc tcagattttc tgtagaaccc
      781 agctttcttg tacaaagtgg tgattcgacc tgcaggcatg caagctcggg ccccccctcg
      841 aggtcgacgg tatcgataag cttgataact tttaaaagaa aaggggggat tggggggtac
      901 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
      961 aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga gaagcttgat
     1021 cgatggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1081 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     1141 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     1201 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtgtc
     1261 gtgacgcgga tccatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     1321 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     1381 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     1441 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     1501 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     1561 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     1621 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcg gatcaggtca
     1681 atgtactaac tacgctttgt tgaagcttgc cggcgatgtt gaaagtaacc ccggtcctat
     1741 gtccagactg gacaagagca aagtcataaa cggcgctctg gaattactca atggagtcgg
     1801 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
     1861 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga
     1921 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
     1981 caacgccaag tcattccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
     2041 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
     2101 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
     2161 actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc
     2221 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg
     2281 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
     2341 aaagtgcgaa agcggcgggc cggcctcgag cgaattccag tacctgccag atacagacga
     2401 tcgtcaccgg attgaggaga aacgtaaaag gacatatgag accttcaaga gcatcatgaa
     2461 gaagagtcct ttcagcggac ccaccgaccc ccggcctcca cctcgacgca ttgctgtgcc
     2521 ttcccgcagc tcagcttctg tccccaagcc agcaccccag ccctatccct ttacgtcatc
     2581 cctgagcacc atcaactatg atgagtttcc caccatggtg tttccttctg ggcagatcag
     2641 ccaggcctcg gccttggccc cggcccctcc ccaagtcctg ccccaggctc cagcccctgc
     2701 ccctgctcca gccatggtat cagctctggc ccaggcccca gcccctgtcc cagtcctagc
     2761 cccaggccct cctcaggctg tggccccacc tgcccccaag cccacccagg ctggggaagg
     2821 aacgctgtca gaggccctgc tgcagctgca gtttgatgat gaagacctgg gggccttgct
     2881 tggcaacagc acagacccag ctgtgttcac agacctggca tccgtcgaca actccgagtt
     2941 tcagcagctg ctgaaccagg gcatacctgt ggccccccac acaactgagc ccatgctgat
     3001 ggagtaccct gaggctataa ctcgcctagt gacaggggcc cagaggcccc ccgacccagc
     3061 tcctgctcca ctgggggccc cggggctccc caatggcctc ctttcaggag atgaagactt
     3121 ctcctccatt gcggacatgg acttctcagc cctgctgagt cagatcagct cctaaagatc
     3181 cgcgtcgtac ggtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3241 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3301 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3361 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3421 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3481 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3541 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3601 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     3661 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3721 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3781 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     3841 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     3901 acaagatggg atcaattcac catgggaata acttcgtata gcatacatta tacgaagtta
     3961 tgctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4021 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4081 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4141 cagtgtggaa aatctctagc agcatctagt tgaatcgcca gtgtgtcgag ctagcagatc
     4201 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     4261 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     4321 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     4381 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     4441 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     4501 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     4561 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     4621 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
     4681 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4741 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4801 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca
     4861 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4921 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4981 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5041 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5101 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5161 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     5221 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5281 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5341 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6061 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6121 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6181 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6241 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6301 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6421 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6481 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6601 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6661 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6841 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6901 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7021 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7081 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7141 ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat tattgactag
     7201 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     7261 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     7321 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     7381 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     7441 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     7501 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     7561 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     7621 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7681 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7741 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7801 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     7861 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     7921 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     7981 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     8041 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     8101 gaacgattcg cagttaatac tggcctgtta gaaacatcag aaggctgtag acaaatactg
     8161 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     8221 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     8281 gacaagatag aggaagagca aaacaaaagt aagaaaaaag cacagcaagc agcaggatct
     8341 tcagacctgg aaattcccta caatccccaa agtcaaggag tagtagaatc tatgaataaa
     8401 gaattaaaga aaattatagg acaggtaaga gatcaggctg aacatcttaa gacagcagta
     8461 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     8521 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     8581 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagaaatcc actttggaaa
     8641 ggaccagcaa agctcctctg gaaaggtgaa ggggcagtag taatacaaga taatagtgac
     8701 ataaaagtag tgccaagaag aaaagcaaag atcattaggg attatggaaa acagatggca
     8761 ggtgatgatt gtgtggcaag tagacaggat gaggattaga acatggaaaa gtttagtaaa
     8821 acaccataag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     8881 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     8941 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     9001 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     9061 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     9121 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     9181 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     9241 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     9301 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     9361 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     9421 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     9481 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     9541 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     9601 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     9661 gtgaacggat ctcgacggta tcggttcggt acaactccca ctagacatat cctactctat
     9721 cattgataga gtgacccttc catagacata tcctactcta tcattgatag agtgaccctt
     9781 ccactaggtt cacttttctc tatcattgat agggagtggt aaacaattga tggacaaaat
     9841 ctatcaatga tagagagtgc atgttactag tattgttctt ctctatcatt gatagtgact
     9901 ttgcctaatc acttagca
//
