LOCUS       sequence-218953-       10340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of the Neugenin 3 transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10340)
  TITLE     Salmon Lab plasmids
REFERENCE   2  (bases 1 to 10340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-Ngn3
FEATURES             Location/Qualifiers
     source          1..10340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    23..41
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    187..205
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    267..291
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(940..964)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        981..1491
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1410..1428
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      1507..1516
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1513..1515,1516..1518,1519..2229)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1513..1515,1516..1518,1519..2229)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1558..1579)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1819..1838)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2166..2187
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2245..2304
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             3373..3744
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    3770..4358
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3823..3843)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4241..4252)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4361..4377)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4362..4378)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(4506..4539)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4559..4739
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4777..4796)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4842..4897
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4896..4915)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5258..5274)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5258..5274)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5271..5293)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5282..5298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5306..5312,5313..5330,5331..5336))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5351..5372
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5431..5626
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5477..5612
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5632..5766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5669..5688)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5723..5742
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5834..5851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6005..6592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6085..6104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6763..7554,7555..7623))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7386..7405
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7624..7728)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7759..8138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8140..8397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8444..8569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9061..9178
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9538..9771
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9956..10000
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    10275..10295
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    10322..10340
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 gacccttcca tagacatatc ctactctatc attgatagag tgacccttcc actaggttca
       61 cttttctcta tcattgatag ggagtggtaa acaattgatg gacaaaatct atcaatgata
      121 gagagtgcat gttactagta ttgttcttct ctatcattga tagtgacttt gcctaatcac
      181 ttagcaactc tatcattgat agagttctag tattagcaag gcctttcctc tatataagga
      241 agttcatttc atttggagaa gatcccccaa gtttgtacaa aaaagcaggc accatgacgc
      301 ctcaaccctc gggtgcgccc actgtccaag tgacccgtga gacggagcgg tccttcccca
      361 gagcctcgga agacgaagtg acctgcccca cgtccgcccc gcccagcccc actcgcacac
      421 gggggaactg cgcagaggcg gaagagggag gctgccgagg ggccccgagg aagctccggg
      481 cacggcgcgg gggacgcagc cggcctaaga gcgagttggc actgagcaag cagcgacgga
      541 gtcggcgaaa gaaggccaac gaccgcgagc gcaatcgaat gcacaacctc aactcggcac
      601 tggacgccct gcgcggtgtc ctgcccacct tcccagacga cgcgaagctc accaagatcg
      661 agacgctgcg cttcgcccac aactacatct gggcgctgac tcaaacgctg cgcatagcgg
      721 accacagctt gtacgcgctg gagccgccgg cgccgcactg cggggagctg ggcagcccag
      781 gcggttcccc cggggactgg gggtccctct actccccagt ctcccaggct ggcagcctga
      841 gtcccgccgc gtcgctggag gagcgacccg ggctgctggg ggccacctct tccgcctgct
      901 tgagcccagg cagtctggct ttctcagatt ttctgtagaa cccagctttc ttgtacaaag
      961 tggtgataat cgaattccac ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     1021 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     1081 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     1141 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     1201 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     1261 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     1321 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     1381 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     1441 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca
     1501 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1561 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1621 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1681 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1741 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1801 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1861 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1921 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1981 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     2041 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2101 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2161 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     2221 ctgtacaagg ctagcggatc aggtcaatgt actaactacg ctttgttgaa gcttgccggc
     2281 gatgttgaaa gtaaccccgg tcctggatcc atgtccagac tggacaagag caaagtcata
     2341 aacggcgctc tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc
     2401 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
     2461 ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg
     2521 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcattccg ctgtgctctc
     2581 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
     2641 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
     2701 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag
     2761 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
     2821 agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg
     2881 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccggcctcg
     2941 agcgaattcc agtacctgcc agatacagac gatcgtcacc ggattgagga gaaacgtaaa
     3001 aggacatatg agaccttcaa gagcatcatg aagaagagtc ctttcagcgg acccaccgac
     3061 ccccggcctc cacctcgacg cattgctgtg ccttcccgca gctcagcttc tgtccccaag
     3121 ccagcacccc agccctatcc ctttacgtca tccctgagca ccatcaacta tgatgagttt
     3181 cccaccatgg tgtttccttc tgggcagatc agccaggcct cggccttggc cccggcccct
     3241 ccccaagtcc tgccccaggc tccagcccct gcccctgctc cagccatggt atcagctctg
     3301 gcccaggccc cagcccctgt cccagtccta gccccaggcc ctcctcaggc tgtggcccca
     3361 cctgccccca agcccaccca ggctggggaa ggaacgctgt cagaggccct gctgcagctg
     3421 cagtttgatg atgaagacct gggggccttg cttggcaaca gcacagaccc agctgtgttc
     3481 acagacctgg catccgtcga caactccgag tttcagcagc tgctgaacca gggcatacct
     3541 gtggcccccc acacaactga gcccatgctg atggagtacc ctgaggctat aactcgccta
     3601 gtgacagggg cccagaggcc ccccgaccca gctcctgctc cactgggggc cccggggctc
     3661 cccaatggcc tcctttcagg agatgaagac ttctcctcca ttgcggacat ggacttctca
     3721 gccctgctga gtcagatcag ctcctaaaga tctgatcaag cttatcgata atcaacctct
     3781 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3841 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3901 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3961 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4021 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4081 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4141 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     4201 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4261 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4321 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg
     4381 ttcgctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4441 aaggggggac tggaagggct aattcactcc caacgaagac aagatgggat caattcacca
     4501 tgggaataac ttcgtatagc atacattata cgaagttatg ctgctttttg cttgtactgg
     4561 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4621 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4681 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4741 catctagttg aatcgccagt gtgtcgagct agcagatctt tttccctctg ccaaaaatta
     4801 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     4861 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     4921 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     4981 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     5041 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     5101 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     5161 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     5221 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5281 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5341 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5401 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
     5461 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5521 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5581 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     5641 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5701 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5761 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5821 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5881 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5941 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6001 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6061 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6121 tgcgctctcc tgttccgacc ctgccgctta cggatacctg tccgcctttc tcccttcggg
     6181 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6241 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6301 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6361 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6421 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6481 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6541 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6601 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6661 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6721 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6781 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6841 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6901 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6961 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     7021 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     7081 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     7141 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     7201 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     7261 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     7321 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     7381 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     7441 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7501 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7561 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7621 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7681 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7741 aaaagtgcca cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg
     7801 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     7861 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     7921 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     7981 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     8041 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     8101 ggcagtacat ctacgtatta gtcatcgcta ttaccatggg aggcgtggcc tgggcgggac
     8161 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     8221 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8281 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8341 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     8401 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     8461 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     8521 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     8581 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     8641 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatactgg
     8701 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     8761 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     8821 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     8881 caaaagtaag aaaaaagcac agcaagcagc aggatcttca gacctggaaa ttccctacaa
     8941 tccccaaagt caaggagtag tagaatctat gaataaagaa ttaaagaaaa ttataggaca
     9001 ggtaagagat caggctgaac atcttaagac agcagtacaa atggcagtat tcatccacaa
     9061 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     9121 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     9181 ggtttattac agggacagca gaaatccact ttggaaagga ccagcaaagc tcctctggaa
     9241 aggtgaaggg gcagtagtaa tacaagataa tagtgacata aaagtagtgc caagaagaaa
     9301 agcaaagatc attagggatt atggaaaaca gatggcaggt gatgattgtg tggcaagtag
     9361 acaggatgag gattagaaca tggaaaagtt tagtaaaaca ccataaggag gagatatgag
     9421 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     9481 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     9541 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     9601 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     9661 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     9721 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     9781 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     9841 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     9901 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9961 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    10021 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    10081 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
    10141 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
    10201 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatct
    10261 agtgattatc ggatcaactt tgtatagaaa agttgggtac aactcccact agacatatcc
    10321 tactctatca ttgatagagt
//
