LOCUS       sequence-218954-       11221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TET-inducible expression of Erythropoietin receptor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11221)
  TITLE     Salmon Lab plasmids submitted
REFERENCE   2  (bases 1 to 11221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCWXPGR-pTF-EpoR
FEATURES             Location/Qualifiers
     source          1..11221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    81..105
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1635..1659)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1676..2186
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2105..2123
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      2202..2211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2208..2210,2211..2213,2214..2924)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2208..2210,2211..2213,2214..2924)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2253..2274)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2514..2533)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2861..2882
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2940..2999
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             4068..4439
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    4465..5053
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4518..4538)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4936..4947)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5056..5072)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5057..5073)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(5201..5234)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5254..5434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5472..5491)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5537..5592
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5591..5610)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5953..5969)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5953..5969)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5966..5988)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5977..5993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6001..6007,6008..6025,6026..6031))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6046..6067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6126..6321
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6172..6307
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6327..6461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6364..6383)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6418..6437
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6529..6546)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6700..7287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6780..6799)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7458..8249,8250..8318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8081..8100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8319..8423)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8454..8833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             8835..9092
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9139..9264
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9756..9873
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10233..10466
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10651..10695
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    10970..10990
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    11017..11035
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    11058..11076
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 actctatcat tgatagagtt ctagtattag caaggccttt cctctatata aggaagttca
       61 tttcatttgg agaagatccc ccaagtttgt acaaaaaagc aggcttcacc atggaccaac
      121 tcagggtggc ccgctggcct cgggttagcc ccctgtgtct cctacttgct ggggcagcct
      181 gggcatcctc acccagcctc ccggacccca agtttgagag caaagccgcc ctgctggcat
      241 cccggggctc cgaagaactt ctatgcttca cccagcgctt ggaagacttg gtgtgtttct
      301 gggaggaagc ggcgaactcc gggatgggct tcaactacag cttctcttac cagctcgaag
      361 gtgaatcaag aaagtcatgt cgcctgcacc aggcgcccac cgtccgcggc tccatgcgtt
      421 tctggtgttc actgccgacc gcggacacgt cgagttttgt gccactggag ctgcaggtga
      481 ccgaggcctc cggatctcct cgctaccacc gcatcatcca tatcaatgaa gtagtgctcc
      541 tggacgcccc cgcggggctg ctggcgcgcc gagcagaaga gggcagccac gtggtgctgc
      601 ggtggctgcc acctcccggg gctcctatga ccacccacat ccgctacgag gtggacgtgt
      661 cagcaggcaa ccgggctggg gggacacaaa gggtggaggt cctggaaggc cgcactgagt
      721 gtgtcctgag caacctgcgg ggcgggacgc gctacacctt cgctgttcga gcacgcatgg
      781 ccgagccgag cttcagcgga ttctggagcg cctggtctga gcccgcgtcg ctactgactg
      841 ctagcgactt ggaccctctc atcttgacgc tgtctctcat tctcgtcctc atctcactgt
      901 tgctgactgt gctggccctg ctgtcccacc gccgggctct gcggcagaag atctggcctg
      961 gcatcccaag cccagagaat gagtttgagg gtctcttcac cacccacaag ggtaacttcc
     1021 agctatggct gttgcaacgc gatggctgtc tgtggtggag cccaagtagc cccttccctg
     1081 aggatccacc tgcccaccta gaggtcctct cagagcgaca ctggggagtg actcaggctg
     1141 gggatgcagg ggcagaggac aaggggccct tactggagcc agtgggcagt gagcgggccc
     1201 aggacaccta cctggtattg gatgaatggt tgctaccccg gtgcccatgc agtgagaacc
     1261 tctctgggcc tggggacagt gtagaccctg cgactatgga tgaaggttca gaaacatctt
     1321 cctgcccctc tgacttggct tcaaagccca ggccagaggg cacctcgcct tccagctttg
     1381 agtacaccat cctggacccc agctctaagc tcctgtgccc tcgggcactg cctcctgagc
     1441 taccccccac tccacctcac ctgaagtacc tgtaccttgt ggtgtccgat tccggcatct
     1501 caacagatta cagctcaggg ggctcccagg gagtccacgg ggactcatct gacggcccct
     1561 actcccaccc ctatgagaat agccttgttc cagacacaga gcctctgcgg cccagctacg
     1621 tggcctgctc ctagacccag ctttcttgta caaagtggtg ataatcgaat tccacggggt
     1681 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     1741 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     1801 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     1861 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     1921 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     1981 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     2041 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     2101 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     2161 accgaatcac cgacctctct ccccaggggg atccaccggt cgccaccatg gtgagcaagg
     2221 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2281 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2341 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2401 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2461 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2521 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2581 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2641 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2701 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2761 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2821 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2881 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggctagc ggatcaggtc
     2941 aatgtactaa ctacgctttg ttgaagcttg ccggcgatgt tgaaagtaac cccggtcctg
     3001 gatccatgtc cagactggac aagagcaaag tcataaacgg cgctctggaa ttactcaatg
     3061 gagtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
     3121 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga
     3181 tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc
     3241 tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc acatcgcgac ggggctaaag
     3301 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
     3361 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
     3421 actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag
     3481 agacacctac caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc
     3541 ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
     3601 aacagctaaa gtgcgaaagc ggcgggccgg cctcgagcga attccagtac ctgccagata
     3661 cagacgatcg tcaccggatt gaggagaaac gtaaaaggac atatgagacc ttcaagagca
     3721 tcatgaagaa gagtcctttc agcggaccca ccgacccccg gcctccacct cgacgcattg
     3781 ctgtgccttc ccgcagctca gcttctgtcc ccaagccagc accccagccc tatcccttta
     3841 cgtcatccct gagcaccatc aactatgatg agtttcccac catggtgttt ccttctgggc
     3901 agatcagcca ggcctcggcc ttggccccgg cccctcccca agtcctgccc caggctccag
     3961 cccctgcccc tgctccagcc atggtatcag ctctggccca ggccccagcc cctgtcccag
     4021 tcctagcccc aggccctcct caggctgtgg ccccacctgc ccccaagccc acccaggctg
     4081 gggaaggaac gctgtcagag gccctgctgc agctgcagtt tgatgatgaa gacctggggg
     4141 ccttgcttgg caacagcaca gacccagctg tgttcacaga cctggcatcc gtcgacaact
     4201 ccgagtttca gcagctgctg aaccagggca tacctgtggc cccccacaca actgagccca
     4261 tgctgatgga gtaccctgag gctataactc gcctagtgac aggggcccag aggccccccg
     4321 acccagctcc tgctccactg ggggccccgg ggctccccaa tggcctcctt tcaggagatg
     4381 aagacttctc ctccattgcg gacatggact tctcagccct gctgagtcag atcagctcct
     4441 aaagatctga tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     4501 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4561 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4621 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4681 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4741 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4801 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4861 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4921 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4981 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5041 gccgcctccc cgcatcgata ccgtcgacct cgaggttcgc tttaagacca atgacttaca
     5101 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     5161 actcccaacg aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca
     5221 ttatacgaag ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     5281 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5341 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5401 agaccctttt agtcagtgtg gaaaatctct agcagcatct agttgaatcg ccagtgtgtc
     5461 gagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     5521 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     5581 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     5641 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     5701 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     5761 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     5821 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     5881 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     5941 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6001 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6061 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     6121 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6181 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6241 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6301 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat
     6361 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6421 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg
     6481 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6541 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6601 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6661 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6721 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6781 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6841 gcttacggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6901 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6961 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7021 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7081 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7141 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7201 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7381 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7441 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7501 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7561 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7621 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7681 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7741 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7801 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7861 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7921 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7981 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8041 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8101 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8161 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8221 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8281 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8341 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
     8461 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     8521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     8581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     8641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     8701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     8761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     8821 cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     8881 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     8941 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9001 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9061 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     9121 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     9181 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     9241 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     9301 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     9361 agcagggagc tagaacgatt cgcagttaat actggcctgt tagaaacatc agaaggctgt
     9421 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     9481 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     9541 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa agcacagcaa
     9601 gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg agtagtagaa
     9661 tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc tgaacatctt
     9721 aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     9781 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     9841 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagaaat
     9901 ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt agtaatacaa
     9961 gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag ggattatgga
    10021 aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta gaacatggaa
    10081 aagtttagta aaacaccata aggaggagat atgagggaca attggagaag tgaattatat
    10141 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
    10201 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
    10261 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
    10321 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
    10381 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
    10441 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
    10501 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
    10561 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
    10621 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    10681 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    10741 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    10801 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    10861 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    10921 tccattcgat tagtgaacgg atctcgacgg tatctagtga ttatcggatc aactttgtat
    10981 agaaaagttg ggtacaactc ccactagaca tatcctactc tatcattgat agagtgaccc
    11041 ttccatagac atatcctact ctatcattga tagagtgacc cttccactag gttcactttt
    11101 ctctatcatt gatagggagt ggtaaacaat tgatggacaa aatctatcaa tgatagagag
    11161 tgcatgttac tagtattgtt cttctctatc attgatagtg actttgccta atcacttagc
    11221 a
//
