LOCUS       sequence-219-exp       11864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 11864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3920
FEATURES             Location/Qualifiers
     source          1..11864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2530..2558)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3306..3326
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(9049..9068)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9050..9068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9075..9092)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9075..9091)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9084..9106)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9233..9688
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9320..9339)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9530..9551
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9715..9819
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9820..9888,9889..10680)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10038..10057)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10851..11439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11593..11610
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(11763..11768,11769..11786,11787..11793)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11801..11817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11806..11828
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11825..11841
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11825..11841
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        join(11862..11864,1..16)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 taaccctcac taaagggaac aaaagctggg ggggatcccg cgtattgcac aacatatttg
       61 acgtgcaaaa tatctagtag cgaaaactat agtaactctt taaatgactt ttgtagcact
      121 gatgccgatt taattaaatt atttattaac agataaaata ttaaaagaaa acacgacaat
      181 tcaaaaaaat tcatttcgta aatcgagccc gtaaatcgac acaagcgcta cagtagtcat
      241 ttaaagaatt actgtttttt cgctacgaga tattttgtgc gtcaaatatg ttgcgcaata
      301 cgcattctta taattttgtg tacccgtagt atatttttgt ctctctgatt tccattgcaa
      361 ttttttaatt ccatattttt ttggtgacaa atttctccgc ttatcaattt ttttttcaac
      421 cacataatta ttaaccccaa aacatcgcag attttcaggt aaaatgccag tcgtgagcgt
      481 tagacctttt tctatgagaa atggtgagca atttttttga agtttttttt tgaaattttt
      541 aatttttcca gaaggcttct caaacgggca taacacgtgg aacgacgccg aattttggaa
      601 accgtgagtt ttcggcaaat aagctaacag tctaagaaac acgaaaaaaa atccaatttt
      661 cttttcaaaa ttcgtgattt tcggatgatt tttgaacttt ttgattaatg ttcccactga
      721 aaatcctcca aatctctaaa aatccgcttc taaaaaagcc tcaaatggcc ttgcgagaat
      781 agacggaatg ttcaaggatg actggtataa aatcggcaaa cgtgttgcca ttcaaacaga
      841 atatctcgaa atttcgcctt cctttttttt aagtgggtcg gatatttaac atacagaaga
      901 gaatagggag agaaaagagg gaaaagaggg gaaaagtctt cttggaaaca acctcgaacc
      961 gcaattaagt ttttgcctca cccttcaatt ttcgtgagaa agttaaaaat ccaaattttt
     1021 ttctcctttt ttaaaaaaag atttcctcct caaaaattca aaggatcgaa ttttttgtta
     1081 cacgcctgcc tcctttttat tacccttgtt cttgatcgca acctcttttc tagattactt
     1141 gaaaaaatgc gctatttttt ttcctttttc aacagagtca atttcaaagg tgctgtggaa
     1201 aaagtttcgg tttttttttg caaaaataat cgaaatttgt tcgtttatac tgcttaaaaa
     1261 atgaccaaaa tttcaagtaa ttcattcgaa aaatcgattt ttttgaaata tacaaaaatt
     1321 ttcaattttt catatctgaa aacacaattt tttcgatttt tcatttttct tggaaaaaac
     1381 acaaaaaaaa actgtattta atttcccact taatactacg atatccaagc cccgcctccg
     1441 cgtattgtac gtgaacacgg cggcccaaaa ttacacacta tctcacacac gttaaatgag
     1501 tgataccgta ctctctctgt tttttctttc attttttcgg cgccgaaaat tttcaatttt
     1561 tggtggttct tttggcggga tttttcggat ttttgtcgcg ttcacacaca cacaccagtt
     1621 ggtgttgcct gtcgtcaccc acccgcccca ctctttgtcc gtgtactgct ttgccatttg
     1681 tgcgcgcgta ccgctgcccg tctgtctttc tcatcatatg cgcctcgttt tgtcgctttt
     1741 tctccatttg cccttcctac atgggtacct gcggttttca tcgaaaatat ccttaaaaat
     1801 tccgaaaaac ctctattttt cgatttttga atcgattttc gcccaaacat aaccattttt
     1861 cagaataaac ctaggggata agatgtcgtc ccgggttcta gaagcgctgc taagggggcc
     1921 ctcgtcgagt cggtcacaat cacctgaaac tccaaaggca gccagtgagg aacgtgaaga
     1981 agaagaaaaa gagtcatctg aacaggtttg attttctttc tggtcaaaaa gatgaaatta
     2041 ttgattttca gccagatact cccaaaacta gcagcgagaa gtctgcaagt cgttcacagt
     2101 cgcccagaga atcgcgggaa gtgagccaag aggtatgttt ttcaaaaatc aataactgat
     2161 cataattttt attgtttggt gaatttaaga aaataatatt cgaaaattcc tctgaattat
     2221 caagattgca gtattaattt cgagaaaaat tgagatattc atagagctat tgtaaatttt
     2281 cttgatttca gactgaaact tcggaaaatc aagagaaaat caaagaaaag gatgacgggg
     2341 atgatcagcc tggcacaccg aacagctata gaagccggga aacttcacca gctccaaaaa
     2401 ggtccaagga gaccaggttt gtcaaaagct tcctgcgatt aattctcatt tcaatttttc
     2461 agagaatcag agtctcctga aaaatcaccg gtagaaaaaa tgagtaaagg agaagaactt
     2521 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     2581 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     2641 tgcactactg gaaaactacc tgttccatgg gtaagtttaa acatatatat actaactaac
     2701 cctgattatt taaattttca gccaacactt gtcactactt tctgttatgg tgttcaatgc
     2761 ttctcgagat acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa
     2821 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtaag
     2881 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtgc tgaagtcaag
     2941 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     3001 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     3061 gcagacaaac aaaagaatgg aatcaaagtt gtaagtttaa acttggactt actaactaac
     3121 ggattatatt taaattttca gaacttcaaa attagacaca acattgaaga tggaagcgtt
     3181 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     3241 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     3301 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     3361 tacaaatgcc cggttcgttc aagatctccc agaaggtctt cagcacgttc cccgtcacga
     3421 tctcctagac ggcgccgaga aagaagctca gaaagaaagc aatccgaaga gccagcaccg
     3481 ctaccagaga aaaagaagaa agagccgctg gatattctac gaacaagaac cggaggagca
     3541 tatattccac ccgccaaact tcgacttatg caacaacaga ttagtgataa gcaaagtgaa
     3601 cagtatcaga gaatgaattg ggaaagaatg aagaaaaaga ttcacggatt ggttaacaga
     3661 gtcaacgcga agaatcttgt tcaaattgtc agagaacttc ttcaagagaa tgtgattcgt
     3721 tcaaagtgag tgagaaaatc gaaggaaaag gaaagaatta atttaatttt tcaggggact
     3781 tctctgccgt gacattattc aagctcaggc tttctcacca ggattctcta acgtctatgc
     3841 agctttggcg gcagttatca actcgaaatt ccctcatgtc ggtgaacttc ttctccgtcg
     3901 tctgattgta cagttcaaaa gaagtttccg tagaaatgac agaggcgtca cggtgaacgt
     3961 gatcaaattc atcgcacatt tgattaatca acaagttgct cacgaagttc ttgcgctgga
     4021 aatcatgatt ctgatgcttg aagaaccaac tgatgattca gttgaagtcg ccattgcgtt
     4081 cctgaaagag tgtggagcaa agcttctgga gattgctcca gcagctctta acagtgtcta
     4141 cgaccgtctt cgtgcaattc tcatggaaac tgaaagatcg gaaaatgcac tggatcgacg
     4201 tattcagtat atgattgaga ctgcaatgca gattcgaaag gacaaatttg cggtaaggta
     4261 gaatatataa atagtttatt agaaaaaaat aaattagaat aatttaaatt cctactagcc
     4321 aatcaggcga cctttttgcg catagttcta ttattgaaaa atttggagaa tttctcatat
     4381 tctcgctcgg aaatctggaa ttcgacgaga tcttctggct tctgtgcagc tgcatcgctt
     4441 tgtgctccct ttctcgcttg tcttctgtgt acaccaagaa ccttgttgag ttcatcaact
     4501 gaatctgtga ctggcttgtt gctcactgga tgcactagac gactgattct cgagaaatca
     4561 gattgagttg cgattagggt gacctagaaa ttgggaataa tacgaacttt tgaaaatatt
     4621 caggaggatt aaaaaaatta ttctcgacaa tcctacaaat ttacttattg caccatgttg
     4681 ctccaacatt tttcattaaa agttaatgaa aaaatgtaga aaatcggaaa ttggcaattt
     4741 tcagaccatt tttaagcatt ttcaaaaaaa aattgcagct gaaataaatg tcattttcag
     4801 ataaatcgag cgattttctg ttgtctgaca ctagttttta gttttaaaaa atgttggaag
     4861 aacatggtgc aataggtaat ttcatagaat ttccatgtgt tttttttcaa ttaaccaatt
     4921 atccaaatct tccaaactca cattttgcgg agctgggcta tcaagaatct gctgcagttt
     4981 tataagacga gcatctctga tatcactgaa aattaatttt taatcaaaac ttgaatatca
     5041 actaaaccca cttattaact ttctcgatct tctgtcgttc ggtacgatga cggtgaagaa
     5101 gccaattgta gtagttgatt tggttcaagt cctttcggtg ttgtacgtca gtgtcctgca
     5161 atgctattta gttataactt aggcctaaga ttcaatttaa tgaagtgatt aaatttgttc
     5221 tctgaacctc ttaagatgat cttttggatt agaaacatat aagacaggtt tacctatcta
     5281 ttaaaaaaca gatcaaaata gatacgacca aatcggataa tccatgccta cctggcatct
     5341 aggaacgtgt tcttagaaga tttcttacgt aatcgtatga agaaataaca atttgatcgt
     5401 tggccagcaa aaatagggtt ttaagtggga tagtgttttt attagctaac cggaaaattt
     5461 tatagttttt ttttgcaaga aaccactgaa aaccccctaa ttgtatacat tttttggagc
     5521 agcttctggt ctttttgagc aataaaattc gataaaacag aatttaagtg taaattgttc
     5581 acatttagtt tctattttat caaattttgt tgctcaaaaa cattcgaagc tgctctaaaa
     5641 aaatgcatta aaaaaggggt tttcagtggt ttttcacatt aaaaaagcta attttaacta
     5701 aaaatccatc atatttccaa ctttgtcaca acaataaaat gctggtcaaa atgtgttcga
     5761 aaaaatgttt ttttttttaa tttttataat ttaaaaatag ttttctttcg ctgggacaca
     5821 tacatttttg ggcgtaaatt ttcagttcaa atttccattt ttacaaccat aatcataaag
     5881 ctacgtctga tctctctcgc acttacctgc gcctgattcg aaagaacaac cgtagccaaa
     5941 agaacaagaa gaacaagcac gtagttgtgg tagtggacgt tcatcacgca atactgacca
     6001 atggtcgtgg ggtctcactt tccgtactat tgagagaggg gagactgaag atggcaattg
     6061 aggacagtgt cttcgacgca cgcatgcatc cataagcata atccaggagg gatggagaga
     6121 aaaatcttgt ttctaagccc ctccctttgt aatacataca catatctaat accgaagaat
     6181 ggctaattga atggacgtca gctgttgctg tagttgccaa ggcatcatcg atgaaataac
     6241 tgaaagaaag aattaaataa ttattgcagg cgtatccggc ggtcattgaa gacttggact
     6301 tgattgagga ggaggatcag atcatccata cacttaattt ggaggatgcg gttgatccgg
     6361 aaaatgggct tagtaagtga ctgaccacac gcggggggca ttaatttaat aaattgaatt
     6421 ccatttcaga tgtgttcaaa ctagatccag aattcgaaaa gaacgaggag gtttatgagg
     6481 agatccgtaa ggaaatcatt ggaaacgccg atatttcgga tgaggatggt ggcgacgagt
     6541 tggatgatga agaagagggt agtgatgtgg aagaggctcc gaagaagact acagagatta
     6601 ttgataatac tgatcagaat tgactgcttt cagaaggtat tcattttgag ttttgggccg
     6661 gcaaatctgt aagttgccgg ttgccgaaaa tttgctgaat ttgccggaaa aaaaaattcc
     6721 ggaatttatt taaaaacttt ttgtaaaaat taaattaaat ttgcaacttt tcagagaagt
     6781 ctacctgaca atgcaatcat ctttggacta ccaagaagct gctcacaaat tgctgaaaat
     6841 gaagattcca gacagcatgc aggtcagcga tgttgcaaag aaaaattttc gaccaaaaaa
     6901 accaaccaat cataaaattt aaaaaaaaac tccgtttttt tctttttttt tatacgagaa
     6961 aaaccaaaaa aatgtatttt tgccaaattc taaaatacta tccccgaaat tttcaatatt
     7021 ttctctttca gaacgaactc tgcgcgatgc ttgtcgattg ttgtgctcaa cagcgtacct
     7081 acgagcgatt ctacggaatg ctcatcgaac gtttctgccg acttcgcctc gaataccagc
     7141 aatactttga aaagctctgc caggacacgt attccacgat tcaccgaatt gacatcacaa
     7201 aactgcggaa tttggctcgc cttattgctc atttgctctc gacggatgct attgactgga
     7261 agattttggc cgatatgaaa atgaccgaag aggacacaac ttcttctggc agaatctata
     7321 ttaaatatat atttaatgaa cttgtggagg cgatgggaat ggttaaactt cattcgagag
     7381 ttactgatcc gtgagtttcc tagagagagt tgttttcgta ttcaattttc cctattttca
     7441 gaactttggc tcattgcttt gttggattat tcccacgaac taatccgaac agcgcacgat
     7501 tttcgatcaa cttcttcaca atgattggat tgggtggttt gacgttggaa cttcgtgaat
     7561 ggctggcaaa gggtctcaag aagaagaagg gaatgctgga tcagttgaag gccgaatcaa
     7621 gctcagattc atcgtcgtct tcggattcgt cagactcgtc tgattcttcg gattctgacg
     7681 attcatccga ctcgtcttca gattcctcat cttcttcaga atcagagcca gaaccaccga
     7741 agaaaaagaa gaagaagaac agtgaagaga gttccaaaaa gaaggaaaaa gagaatattg
     7801 gtcgacggga tcgtggagac aagagagctg aacgtcatcg tgatcaaagt gtggagaaca
     7861 aggacaagga tcgtcgacgt cgccaggatt ctgacgaaaa tcgtcggcca gaacgaggag
     7921 atgaccgcaa ggatcggagt aaagatcgtc gtcgtcaaga ctcggatgat gaggatcgga
     7981 aaggtcgtga acgtcgggaa gattcagggg aaagacgtcg cggagatcgg gatcgacgtg
     8041 atcgaaacaa ggatcaggag gatcaccgtg aagatcgccg tgaccgaagc aaggatcgtg
     8101 aggatcgacg tgatcgccgt cgtcatgact ctgatgatga tcgtaaaact cgtcgggata
     8161 gaagtgaaga gcgaggagga cgtcgtcgtg aagtggaatc ggatgatcga cgccgacgtc
     8221 gttgaatttt caaattttaa atactgaata tttgtttttt ttcctattat ttatttattc
     8281 tctttgtgtt ttttttcttg ctttctaaaa aattaattca atccaaatct aaacattttt
     8341 ttttctcttt ccgtctccca attcgtattc cgctcctctc atctgaacac aatgtgcaag
     8401 tttatttatc ttctcgcttt catttcatta ggacgtgggg ggaattggtg gaagggggaa
     8461 acacacaaaa ggatgatgga aatgaaataa ggacacacaa tatgcaacaa cattcaattc
     8521 agaaatatgg aggaaggttt aaaagaaaac ataaaaatat atagaggagg aaggaaaact
     8581 agtaaaaaat aagcaaagaa attaggcgaa cgatgagaat tgtcctcgct tggcaaatgc
     8641 gaatccgtat ggagaggcac gtttggcgaa ggcaaatgtt cggtatggag atctgtaaaa
     8701 atttttaagt tgaaatttgg tgttgctctt ttacaaaatt ttccgatttt cgcttgaaat
     8761 tacggtgcca ggtctcgaca cgtcttccaa tttttcaaat tcaaaagagc ctttaatggg
     8821 ctgtagttgc taatttctcg tttttgaaaa tttttcttcc gtttaatcga aatttgatgt
     8881 attttattta tgattttcaa taaatttcaa agaaactggt gaaaactcgg aaaattgtga
     8941 actacagtaa tccaatcctt aaaggcgcac accttttaaa tgtccgcccc aatacgatat
     9001 ttttttaaga ttctagagcg gccgccaccg cggtggagct ccaattcgcc ctatagtgag
     9061 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     9121 acccaactta atcgccttgc agcacatccc cccttcgcca gctggcgtaa tagcgaagag
     9181 gcccgcaccg atcgcccttc ccaacagttg cgtagcctga atggcgaatg ggacgcgccc
     9241 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9301 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9361 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9421 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     9481 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     9541 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     9601 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     9661 tttaacaaaa tattaacgtt tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga
     9721 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9781 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9841 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9901 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9961 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    10021 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
    10081 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    10141 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataagcatg
    10201 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10261 gctttttttc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10321 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10381 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10441 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10501 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10561 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacgggcag tcaggcaact
    10621 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10681 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10741 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10801 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10861 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10921 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10981 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
    11041 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    11101 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    11161 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11221 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11281 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11341 gggaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11401 tttttgtgat gctcgtcagg ggggccgagc ctatggaaaa acgccagcaa cgcggccttt
    11461 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11521 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11581 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    11641 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
    11701 aaagcgggca gtgagcgcaa cgcaattaat gtgagttacc tcactcatta ggcaccccag
    11761 gctttacact ttatgcttcc ggctcctatg ttgtgtggaa ttgtgagcgg ataacaattt
    11821 cacacaggaa acagctatga ccatgattac gccaagctcg gaat
//
