LOCUS       sequence-21900-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Paoletti AC, Parmely TJ, Tomomori-Sato C, Sato S, Zhu D, Conaway RC,
            Conaway JW, Florens L, Washburn MP
  TITLE     Quantitative proteomic analysis of distinct mammalian Mediator 
            complexes using normalized spectral abundance factors.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Dec 12. 103(50):18928-33.
  PUBMED    17138671
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Dec 12. 103(50):18928-33."
COMMENT     Sequence Label: pET19b-hMED9
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..60
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             76..90
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(594..612)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      608..655
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taactttaag aaggagatat accatggggc catcatcatc atcatcatca tcatcatcac
       61 agcagcggcc atatcgacga cgacgacaag catatgctcg agatggcctc tgctggggtg
      121 gcagccgggc gacaggcgga ggatgtattg ccgccaacgt ccgaccagcc gctgcctgac
      181 accaagccgc tgccgcctcc tcagccgccg ccggtccctg cgcctcaacc gcagcagtcg
      241 ccggcgccac ggcctcagtc acctgcccgc gcgagggagg aagagaacta ctccttttta
      301 cctttggttc acaacatcat caaatgcatg gacaaggaca gcccggaggt ccaccaggac
      361 ctgaacgccc tcaaaagcaa gttccaggag atgcgcaagc tcatcagcac catgcccggc
      421 atccacctga gccccgaaca gcagcagcag cagctgcaga gcctccggga gcaagtcagg
      481 accaagaatg agcttctgca aaagtacaag agcctctgca tgttcgaaat ccccaaggag
      541 tagggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      601 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      661 aggaggaact atatccggat atcccgcaag aggcccggca gtaccggcat aaccaagcct
      721 atgcctac
//
