LOCUS       sequence-21903-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson 
            SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC
  TITLE     A set of consensus mammalian mediator subunits identified by 
            multidimensional protein identification technology.
  JOURNAL   Mol Cell. 2004 Jun 4. 14(5):685-91.
  PUBMED    15175163
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2004 Jun 4. 14(5):685-91."
COMMENT     Sequence Label: F-MED28
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(753..770)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 agagctctct ggctactaga gaaccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcatgc cagactacaa ggacgatgat gacaagctcg
      121 agatggcggc tccactaggg ggtatgtttt ctgggcagcc acccggtccc cctcaggccc
      181 cgccgggcct tccgggccaa gcttcgcttc ttcaggcagc tccaggcgct cctagacctt
      241 ccagcagtac tttggtggac gagttggagt catctttcga ggcttgcttt gcatctctgg
      301 tgagtcagga ctatgtcaat ggcaccgatc aggaagaaat tcgaaccggt gttgatcagt
      361 gtatccagaa gtttctggat attgcaagac agacagaatg ttttttctta caaaaaagat
      421 tgcagttatc tgtccagaaa ccagagcaag ttatcaaaga ggatgtgtca gaactaagga
      481 atgaattaca gcggaaagat gcactagtcc agaagcactt gacaaagctg aggcattggc
      541 agcaggtgct ggaggacatc aacgtgcagc acaaaaagcc cgccgacatc cctcagggct
      601 ccttggccta cctggagcag gcatctgcca acatccctgc acctctgaag ccaacgtgag
      661 caaagggcag aggcagttgg cctatgagtg ggctgatgcg tgaggatccg gtaccgtcga
      721 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
      781 atctgttgtt t
//
